| Project/Area Number |
14571558
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAKATA Masahiro Osaka University, Hospital, Associate professor, 医学部附属病院, 助教授 (10260639)
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| Co-Investigator(Kenkyū-buntansha) |
TASAKA Keiichi Osaka University, Graduate School of Medicine, Associate professor, 医学系研究科, 助教授 (50155058)
OHMICHI Masahide Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (10283764)
武田 卓 大阪大学, 医学系研究科, 助手 (20301260)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | ES cell / Oct3 / 4 / hCG / trophoblast / GLUT1 / HIF / Id-1 / BMP-4 / trophoblast / BMP4 / 絨毛細胞 |
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| Research Abstract |
Glucose transporter 1 (GLUT1) plays an important role in the transport of glucose in the placenta. The study was designed to test whether hypxoxia inducible factor (HIF)-1α is involved in hypoxia-induced activation of GLUT1 expression using trophoblast-derived human BeWo and rat Rcho-1 cells as models. Our data demonstrated that GLUT1 is up-regulated under hypoxic conditions in the placental cell lines through HIF-1α interaction. It is published in journal of endocrinology (145-154; 183, 2004).
The next study was designed to investigate the effect of low oxygen conditions on RhoA expression and HIF-1α in trophoblast cells isolated from human placenta and in choriocarcinoma cell lines. Our findings suggested that up-regulation of RhoA induced by low oxygen conditions may play an important role in regulation of HIF-1α expression in trophoblast cells, which is published in J.Clinical Endocrinol Metab (in press.)
During early pregnancy, trophoblast cells are exposed to relative low oxygen tension. Taken together from our two published data, HIF-1α may play an important role in the regulation of placental gene expression under physiological hypoxic condition during trophoblast cell differentiation
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