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Research on the new non-invasive prenatal diagnostic technique for chromosome abnormalities.

Research Project

Project/Area Number 14571579
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionIwate Medical University

Principal Investigator

FUKURSIMA Akimune  Iwate Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20208937)

Co-Investigator(Kenkyū-buntansha) OYAMA Rio  Iwate Medical University, School of Medicine, Research Associate, 医学部, 助手 (20291619)
SHOJI Thdahiro  Iwate Medical University, School of Medicine, Research Associate, 医学部, 助手 (00337148)
SUGLYAMA Thni  Iwate Medical University, School of Medicine, Professor, 医学部, 教授 (40162903)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsFetal nucleated red blood cells / Laser Scanning Cytometer / Masgnetic cell sorter / Nested-PCR / nested-PCR / magnetic cell sorter / Laser Scanning cytometry
Research Abstract

The object of the present study was to attempt to apply Laser Scanning Cytometer (LSC) to automated detection of fetal nucleated red blood cells (FNRBC) in maternal peripheral blood. 1) Fetal cell isolation : We investigate the effectiveness of a relatively easy and efficient method of sorting fetal nucleated red blood cells (FNRBCs) from maternal peripheral blood, particularly during early gestation periods, by a combination of specific gravity centrifugation and magnetic cell sorter (MACS). Without prior knowledge of the sex of each fetus, we determined gender by analyzing a Y-chromosome specific sequence by nested-PCR, using 10 ml of the peripheral blood of healthy primigravida women at different stages of gestation (first trimester: n17, second trimester : n=13, and third trimester : n=19). The results of this prenatal sex determination were compared to the sex of newborns. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the pres … More ent method during the first trimester was 81.8%, 100%, 100% and 75%, respectively; during the second trimester, 50%, 80%, 80%, and 50%, respectively; and during the third trimester, 63.6%, 25%, 53.8% and 33.3%, respectively. 2) LSC technique: A venous blood sample (lOmI) was collected from 29 women carrying a male fetus between 5 and 38 weeks of gestation who gave informed consent to participate in the present study. Twenty-four of the blood samples were taken from normal pregnant woman, while 5 were taken from women hospitalized to inhibit labor. Eukaryotic cells were promptly separated by gravity centrifugation. Then, cells negative for CD45 monoclonal antibody and positive for glycophorin A monoclonal antibody (GA) were selectively collected using magnetic-activated cell sorter (MACS) to obtain FNRBC. FNRBCs were determined by fluorescence in situ hybridization (FISH) Y specific probes. FNRBCs were found with an average count of 9.7±4.2 per 10 ml of venous blood. We found no relationship between stage of pregnancy and number of FNRBC. There was a good correlation between results of manual and automated screening for the 29 samples examined (R2= 0.843). These results show this prenatal sex determination method has a highly accurate diagnostic rate during the first trimester, suggesting that it could be developed as a practical, non-invasive prenatal diagnostic technique for use during early gestation periods. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (13 results)

All 2003 2001 Other

All Journal Article (11 results) Publications (2 results)

  • [Journal Article] 妊婦末梢血中の胎児由来細胞検出-磁気細胞分離システムの臨床応用-2003

    • Author(s)
      福島明宗 他
    • Journal Title

      産婦人科の世界 55・春季増刊号

      Pages: 191-198

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 母体末梢血の胎児由来細胞の検出と分析の自動化2003

    • Author(s)
      福島明宗 他
    • Journal Title

      日本新生児学会雑誌 39(4)

      Pages: 647-652

    • NAID

      10012624690

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Ninnpu massyouketsucyu no taijiyuraisaibou kennsyutsu.2003

    • Author(s)
      Akimune Fukushima, Saburo Horiuchi
    • Journal Title

      Sanfujinnka no Sekai 55

      Pages: 191-198

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Botai massyouketsu no taijiyurai saibou no kensyutsu to bunseki no jidouka.2003

    • Author(s)
      Akimune Fukushima
    • Journal Title

      Nihon Shinseiji Gakkai Zasshi 39

      Pages: 647-652

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 妊婦末梢血中の胎児由来細胞検出-磁気細胞分離システムの臨床応用-2003

    • Author(s)
      福島明宗 他
    • Journal Title

      産婦人科の世界 55・春期増刊号

      Pages: 191-198

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The application of magnetic cell sorter (MACS) to detect fetal cells in maternal peripheral blood.2001

    • Author(s)
      Akimune Fukushima et al.
    • Journal Title

      The Journal of Obstetrics and Gynecology Research 27(3)

      Pages: 155-162

    • NAID

      10015766480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes of the quantity of fetal nucleated red blood cells and fetal DNA in the peripheral blood of pregnant women.2001

    • Author(s)
      Yuko Yaegashi, Akimune Fukushima
    • Journal Title

      岩手医学雑誌 53(5)

      Pages: 337-345

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 母体血中有核赤血球による非侵襲的出生前診断-磁気細胞分離システム(MACS)とレーザースキャニングサイトメトリー(LSC法)の応用2001

    • Author(s)
      槍澤ゆかり, 福島明宗
    • Journal Title

      産婦人科の世界 53(9)

      Pages: 997-984

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The application of magnetic cell sorter (MACS) to detect fetal cells in maternal peripheral blood.2001

    • Author(s)
      Akimune Fukushima, Yukari Utsugizawa, Yuko Wada, Noriko Mizusawa, Saburo Horiuchi, Teruo Kagabu
    • Journal Title

      The Journal of Obstetrics and Gynecology Research 27

      Pages: 155-162

    • NAID

      10015766480

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes of the quantity of fetal nucleated red blood cells and fetal DNA in the peripheral blood of pregnant women.2001

    • Author(s)
      Yuko Yaegashi, Akimune Fukushima
    • Journal Title

      The Journal of the Iwate Medical Association 53

      Pages: 337-345

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Botaikechyu yukakusekkekkyu niyoru hishinnsyuuteki Syusseizenshindan.2001

    • Author(s)
      Yukari Utsugisawa, Akimune Fukushima
    • Journal Title

      Sanfujinka no Sekai 53

      Pages: 997-984

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 福島明宗 他: "妊婦末梢血中の胎児由来細胞検出-磁気細胞分離システムの臨床応用-"産婦人科の世界. 55・春期増刊号. 191-198 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福島明宗 他: "母体末梢血の胎児由来細胞の検出と分析の自動化"日本新生児学会雑誌. 39(4). 647-652 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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