| Project/Area Number |
14571593
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
HASHIMOTO-TAMAOKI Tomoko Hyogo College of Medicine, Hyogo College of Medicine, Faculty of Medicine, Professor (10172868)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Yoshiyuki 兵庫医科大学, Faculty of Medicine, Associate Professor (60148658)
TACHIBANA Hisao 兵庫医科大学, Faculty of Medicine, Professor (80124949)
OKAMURA Haruki 兵庫医科大学, Faculty of Medicine, Professor (60111043)
MORINAGA Tomonori 兵庫医科大学, Faculty of Medicine, Research Associate (10351818)
NAKANO Yoshiro 兵庫医科大学, Faculty of Medicine, Research Associate (30360267)
皆川 京子 兵庫医科大学, 医学部, 助手 (80268557)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | IL-18 / Neuronal differentiation / Cell damage / Transcription activity / Brain damage / Premature infant / 未熟児脳障害 / 胚性細胞 / 神経分化阻害 / アポトーシス / プロモーター活性 / レチノイン酸 |
|---|
| Research Abstract |
To clarify the role of the high concentration of IL-18 to develop cerebral palsy in premature infants, we used mouse P19 embryonal carcinoma cells as a model for early neuronal differentiation. During differentiation of P19 cells, increased expression of IL-18mRNA transcribed from the first exon and IL-18 precursor protein was observed. However, exogenous mature IL-18 (active form) showed no effects on cell damage or neuronal differentiation. We then examined whether exogenous mature IL-18 influences human immature endothelial cells. cDNA microarray analysis showed that several genes were up- or down-regulated, suggesting that mature IL-18 may target immature endothelial cells, resulting in severe brain damages.
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