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The study of cell membrane ion channels involving in automaticity of human uterine smooth muscle cells.

Research Project

Project/Area Number 14571596
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFukuoka University

Principal Investigator

INOUE Yoshihito  Fukuoka University, School of Medicine, Lecturer, 医学部, 講師 (20260698)

Co-Investigator(Kenkyū-buntansha) KAWARABAYASHI Tatsuhiko  Fukuoka Univ., School of Medicine, Professor, 医学部, 教授 (30142350)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Keywordsuterine smooth muscle. / hyperpolarization-activated inward current / T-type calcium channel / voltage clamp / α1 subunit / ヒト子宮平滑筋 / T-type Ca^<2+> channel / 収縮頻度調整 / α1 subunit / 過分極誘発性内向き電流 / 単離細胞
Research Abstract

The aim of this study was to find the membrane channels which involves in automaticity of human myometrium. For the beginning, we tried to record hyperpolarization-induced inward current which was recorded in rat myometrium using patch clamp methods, but it failed. So we evaluated the difference in the expression of mRNA of two types of calcium channels between longitudinal and circular muscle layer of rat mayometrium during pregnancy. Changes in the expressions of the mRNA encoding L-type (α1C) and T-type (α1G, α1H, and all) calcium channels in longitudinal and circular smooth muscle cells of the rat myometrium were examined using a comparative kinetic RT/PCR method. During the course of pregnancy, α1C mRNA expression showed an N-shaped change in longitudinal muscle, but simply increased after mid pregnancy in circular muscles. The mRNAs for α1G and α1H, but not that for all, were expressed in both longitudinal and circular smooth muscle. In longitudinal muscle, the changes in α1H mRNA was similar to that in α1C mRNA during gestation, but the expression of α1G mRNA changed significantly only at term (day 22). In circular muscle, α1H mRNA expression was stable at any stage during pregnancy, but α1G mRNA significantly increased on day 15 and at term. No relationship was observed between voltage-dependent calcium-channel mRNA expressions and either proliferation or hypertrophy of circular muscle during pregnancy. These results show, (a)that during pregnancy, the expression levels of L-type channels change dynamically, and it may contribute directly to the regulation of cell excitability, and (b)that the T-subtype that increases during pregnancy differs between longitudinal and circular muscle cells, although their functions remain unclear.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All 2005 2003 2002

All Journal Article (7 results)

  • [Journal Article] Differential expression of L-and T-type calcium channels between longitudinal and circular muscles of the rat myometirium during pregnancy2005

    • Author(s)
      Ohkubo T, Inoue Y, Kawarabayashi T, Kitamura K
    • Journal Title

      Gynecologic and Obstetric Investigation 59

      Pages: 80-85

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential expression of L-and T-type calcium channels between longitudinal and circular muscles of the rat myometrium during pregnancy.2005

    • Author(s)
      Ohkubo T, Kawarabayashi T, Inoue Y, Kitamura K
    • Journal Title

      Gynecologic and Obstetric Investigation 59

      Pages: 80-85

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential expression of L- and T-type calcium channels between longitudinal and circular muscles of the rat myometirium during pregnancy2005

    • Author(s)
      Ohkubo T, Inoue Y, Kawarabayashi T, Kitamura K
    • Journal Title

      Gynecologic and Obstetric Investigation 59

      Pages: 80-85

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Amino acid-permeable anion channels in early mouse embryos and their possible effects on cleavage2003

    • Author(s)
      Sonoda M, Okamoto F, Kajiya H, Inoue Y et al.
    • Journal Title

      Biology of Reproduction 68

      Pages: 947-953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amino acid-permeable anion channels in early mouse embryos and their possible effects on clevage.2003

    • Author(s)
      Sonoda M, Okamoto F, Kajiya H, Inoue Y Honjo K, Sumii Y, Kawarabayasi T, Okabe K
    • Journal Title

      Biology of Reproduction 68

      Pages: 947-953

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Diversity of inhibitory responses to β2-stimulants shown by term-pregnant human myometria in vitro is partly due to differences in receptor density2002

    • Author(s)
      Sakakibara T, Inoue Y, Uzue S et al.
    • Journal Title

      American Journal of Obstetrics and Gynecology 186

      Pages: 997-1004

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Diversity of inhibitory responses to β2-stiinulants shown by term-pregnant human myometria in vitro is partly due to differences in receptor density.2002

    • Author(s)
      Sakakibara T, Inoue Y, Uzue S, Tsukamoto T, Kobayashi M, Kojima M, Akahane M, Kitamura K, Kawarabayashi T
    • Journal Title

      Am J Obstet Gynecol 186

      Pages: 997-1004

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2002-04-01   Modified: 2016-04-21  

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