| Project/Area Number |
14571599
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Japanese Foundation For Cancer Research |
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Principal Investigator |
HIRAI Yasuo JAPANESE FOUNDATION FOR CANCER RESEARCH, CANCER INSTITUTE, RESEARCH FELLOW, 癌研究所・細胞生物部, 研究員 (00260076)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | ENDOCERVICAL ADENOCARCINOMAS / ENDOMETRIAL ADENOCARCINOMA / GLASSY CELL CARCINOMA / CONVENTIONAL CGH / ARRAY-BASED CGH / MICROSATELLITE INSTABILITY / PTEN / HPV INFECTION / 子宮頸部腺癌 / HPV感染 / TCFβ typeII Receptor / TCF βtype II Recetpor |
|---|
| Research Abstract |
We analyzed the mutational status of the TGF βRII, BAX, and PTEN/MMAC1 genes as well as microsatellite instability (MI) in consecutive cases of endocervical and endometrial adenocarcinomas of the uterus operated on at our Institute. To examine copy number losses and/or amplifications at the chromosomal region bearing all the genes, we used comparative genomic hybridization (CGH) analysis (both conventional and array-based). CGH analysis provides a genome-wide overview about tumor-associated genomic imbalances. Among the tumors, a few showed the MI+ phenotype in endocervical adenocarcinoma. Also, few tumors demonstrated a significant mutation with a definite amino acid change in PTEN/MMAC1 gene, which is prominent in endometrial adenocarcinoma. CGH analysis demonstrated several promising oncogenes which may have a role in multi-step carcinogenesis of uterine endocervical and endometrial adenocarcinomas.
Additional explorations are needed to elucidate the cytogenetic mechanisms of multi- step carcinogenesis in endocervical and endometrial adenocarcinomas of the uterus.
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