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A transfer study of human tonsillar lymphocytes into NOD/LtSz-scid/scid mice

Research Project

Project/Area Number 14571619
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionShimane University, School of Medicine

Principal Investigator

KAWAUCHI Hideyuki (2003)  Shimane University, School of Medicine, Professor, 医学部, 教授 (50161279)

岩元 純一 (2002)  島根医科大学, 医学部, 助手 (20284036)

Co-Investigator(Kenkyū-buntansha) IWAMOTO Jun-ichi  Shimane University, School of Medicine, Assistant, 医学部, 助手 (20284036)
ISHIMITSU Ryotaro  Shimane University, School of Medicine, Assistant, 医学部, 助手 (90301291)
KATAOKA Shingo  Shimane University, School of Medicine, Assistant, 医学部, 助手 (60152667)
川内 秀之  島根医科大学, 医学部, 教授 (50161279)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordstonsillar lymphocytes / NOD-acid mice / mucosal immunity / lymphocyte homing, inductive site / effector site / common mucosal immunity / NOD-scid / IL-2RγKOマウス
Research Abstract

A sophisticated common mucosal immunity system is provided in the nasopharyngeal mucosal linings, as previously shown by a large number of studies on animals and humans. Homing theory is now settled down for explaining recruitments of primed lymphocytes from inductive site to various distant mucosal sites. However, it still remains to be investigated how these precursor cell B and T cells recruit to the distant mucosal sites and how final effector antibody-producing B cells differentiate and conduct a clonal expansion. To challenge this issue, we have done a transfer study of human tonsillar lymphocytes into NOD/LtSz-scid/scid mice and obtained successful results in which human leukocytes are engrafted in the spleens and other non-lymphoid organs such as lung and liver. In a flow cytometric analysis, human helper/inducer and suppressor/cytotoxic T cells were detected in various degrees in spleens of NOD/LtSz-scid/scid mice at a 4-week interval after human tonsillar mononuclear cells we … More re inoculated intraperitoneally into the mice. However, there was no engraftment of human tonsillar cells in CB-17 acid mice. An immunohistochemical study revealed that human leukocytes were present in the spleen, thymus, lung, and liver, but not in normal nasopharyngeal or tubotympanal mucosae. In NOD-acid mice engrafted with human tonsillar lymphocytes obtained from patients carrying β Streptococcus, M-protein specific human IgG and IgA antibody activities were detected in the sera of these mice. Upon an intranasal immunization of M-protein and cholera toxin to these mice, M-protein specific human IgA antibody titers in nasal washings were higher than those of mice without intranasal challenge of M-proteins. In an immunostaining study, human T and B cells were recruited to the nasal mucosae around the nasopharyngeal lymphoreticular tissue of those mice having higher M-protein specific human IgA antibody titers. These results may allow us to use this model as a promising tool for evaluating the lymphocyte homing and mechanism of the final differentiation of recruiting precursor lymphocytes for mounting antigen-specific immune response in peripheral mucosal, sites such as the nasopharynx and tubotympanum. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Hideyuki Kawauchi: "Mucosal Immunity of Nasopharynx"Rhinology. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 川内 秀之: "上気道粘膜の生体防御機構と炎症性疾患の治療戦略"耳鼻咽喉科免疫アレルギー. 21・4. 1-16 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "A Transfer Study of Human Tonsillar Lymphocytes into NOD/LtSz-scid/scid Mice"Recent Advances in Otitis Media. 423-424 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hisashi Ohga: "S-carboxymethyl cysteine(S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils"Recent Advances in Otitis Media. 424-425 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryotatro Ishimitsu: "NKT cells are dispensable in the abrogation of oral tolerance by prost aglandin E"European Journal of Immunology. 33. 183-193 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 川内 秀之: "呼吸器における粘膜免疫誘導の分子機構と呼吸器アレルギーの抑制"アレルギー・免疫. 9・12. 110-113 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Basic Immunology of Nasal and Paranasal Sinus Mucosa and Our Treatment Strategy with an Immuno pharmacological Drug (Suplatast Tosilate) of the Chronic Infective Rhinosinusitis Coupled with Nasal Allergy"Nasal Polyposis. 28-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Mucosal Immunity of Nasopharynx"Rhinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Molecular approach for induction of mucosal immunity in respiratory tract and regulatory mechanism of allergic disorders"Allergy and Immunology. 21(4). 1-16 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "A Transfer Study of Human Tonsillar Lymphocytes into NOD/LtSz-scid/scid Mice"Recent Advances in Otitis Media. 423-424 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hisashi Ohga: "S-carboxymethyl cysteine(S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils"Recent Advances in Otitis Media. 424-425 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryotatro Ishimitsu: "NKT cells are dispensable in the abrogation of oral tolerance by prostaglandin E"European Journal of Immunology. 33. 183-193 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Immunological defense system of upper respiratory tract and treatment strategy for inflammatory diseases"Journal of Japan of Immunology & Allergology in Otolaryngology. 9(12). 110-113 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Mucosal Immunity of Nasopharynx"Rhinology. In Press.

    • Related Report
      2003 Annual Research Report
  • [Publications] 川内秀之: "上気道粘膜の生体防御機構と炎症性疾患の治療戦略"耳鼻咽喉科免疫アレルギー. 21・4. 1-16 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hideyuki Kawauchi: "A Transfer Study of Human Tonsillar Lymphocytes into NOD/LtSz-scid/scid Mice"Recent Advances in Otitis Media. 423-424 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hisashi Ohga: "S-carboxymethyl cysteine(S-CMC) has a suppressive effect on the Hydrogen Peroxide Production of Neutrophils"Recent Advances in Otitis Media. 424-425 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ryotaro Ishimitsu: "NKT cells are dispensable in the abrogation of oral tolerance by prostaglandin E"European Journal of Immunology. 33. 183-193 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 川内秀之: "呼吸器における粘膜免疫誘導の分子機構と呼吸器アレルギーの抑制"アレルギー・免疫. 9・12. 110-113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hideyuki Kawauchi: "Basic Immunology of Nasal and Paranasal Sinus Mucosa and Our Treatment Strategy with an Immunopharmacological Drug(Suplatast Tosilate)of the Chronic Infective Rhinosinusitis Coupled with Nasal Allergy"Nasal Polyposis. 28 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hideyuki Kawauchi: "T-cell mucosal Immunity of the nasopharynx -animal experiments -"New Frontiers in Immunobiology. (In Press). (2004)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ryotaro Ishimitsu: "Dichotomouse effect of a tradirional Japanese medicine, Bu-zhong-yi-qi-tang on allergic asthma in mice"International Immunopharmacology. 1. 857-865 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ryotaro Ishimitsu: "Overexpression of IL-15 in vivo enhances Tc1 response, which inhibits allergic inflammation in a murine model of asthma"Journal of Immunology. 166. 1991-2001 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hideyuki Kawauchi: "Mucosal immunity of nasopharynx-an experimental study in TCR-transgenic (OVA23-3) mice"Rhinology. Supplement 15. 56 (1999)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石光亮太郎: "当科における鼻副鼻腔真菌症の治療経験"日本耳鼻咽喉科感染症研究会会誌. 17・1. 143-148 (1999)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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