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The heat shock protein(HSP) was investigated as the pathogenesis of focal infection with tonsil by the SCID mouse model

Research Project

Project/Area Number 14571629
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionWakayama Medical University

Principal Investigator

FUJIHARA Keiji  Wakayama Medical University, Department of Otorhinolaryngology, Assistant Professor, 講師 (80326371)

Co-Investigator(Kenkyū-buntansha) KUKI Kiyonori  Wakayama Medical University, Department of Otorhinolaryngology, Associate Professor, 助教授 (40169975)
YAMANAKA Noboru  Wakayama Medical University, Department of Otorhinolaryngology, Chief Professor, 教授 (10136963)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsPustulosis palmaris et plantaris / Tonsillar focal infection / Heat shock protein / Antibody for HSP60 / SCID mouse / ELISA method / Immuno histological stain / DnaK抗体 / GroEL抗体
Research Abstract

<Introduction>
In this study, we investigated the heat shock protein(HSP) as the relationship between tonsils and pustulosis palmaris et plantaris(PPP). We engrafted human cells onto severe combined immunodifficiency(SCID) mice, together with the human skin.
<Material, Method and Results>
1.We got the tonsil, peripheral blood and normal skin of sole from PPP patient with permission. We transplanted tonsillar or peripheral lymphocytes together with the sole skin into SCID mice. We examined the anti-bacterial HSP antibody by ELISA. DnaK is bacterial HSP 70. GroEL is bacterial HSP65. HSP-60 is also bacterial HSP. We got high titer of anti-DnaK antibody, anti-GroEL antibody and anti-HSP60IgG antibody just in the mice transplanted tonsillar lymphocytes with auto-sole skin.
2.The engrafted skins were immuno-stained. The infiltrated lymphocytes were mainly CD4+ and CD8+ T lymphocytes.
3.We got the high titer of anti HSP60IgG antibody, injected the HSP60 recombinant to the mice transplanted the tonsil lymphocytes.
<Discussion>
We already reported that tonsillar lymphocytes directly infiltrated into human sole skin in human skin/SCID mouse chimera model. In this study, we found high titer of anti-HSP antibody in the same model. It seemed that the tonsillar lymphocytes had already sensitized by bacterial HSP in crypts of tonsil. The lymphocytes may react with the HSP in human sole skin. The tonsillar lymphocytes of PPP may react with human sole skin by cell-immunity and humoral-immunity. We emphasized that the HSP may be one of the pathogen of PPP. The anti-HSP antibody may be the help of pre-operation diagnosis of focal infection of tonsil with PPP.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (32 results)

All 2005 2004 2003 2002 2001 2000 Other

All Journal Article (21 results) Book (1 results) Publications (10 results)

  • [Journal Article] Tonsillitis index : an objective tool for quantifying the indications for tonsillectomy for recurrent Acute tonsillitis.2005

    • Author(s)
      K Fujihara, H Goto, PJ Koltai, M Hiraoka, M Hayashi, M Hotomi, S Tamura, K Kuki, N Yamanaka.
    • Journal Title

      International Journal of otorhinolaryngology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The indication of the operation for recurrent tonsillitis and tonsillar focal infection (pustulosis palmaris et plantaris)2005

    • Author(s)
      K Fujihara, H Goto, M Hayashi, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of stomato-pharyngology 17(2)

      Pages: 205-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The indication of operation for recurrent (habitual) tonsillitis.2005

    • Author(s)
      K Fujihara, N Yamanaka
    • Journal Title

      Pediatrics of Japan. 46(4)

      Pages: 585-592

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tonsillitis index : an objective tool for quantifying the indications for tonsillectomy for recurrent Acute tonsillitis.2005

    • Author(s)
      K.Fujihara, H.Goto, PJ Koltai, M Hiraoka, M Hayashi, M Hotomi, S Tamura, K Kuki, N Yamanaka
    • Journal Title

      International Journal of otorhinolaryngology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 扁桃病巣感染症における熱ショック蛋白に関する研究〜第3報〜2004

    • Author(s)
      林 正樹, 藤原啓次, 九鬼清典, 山中 昇, 他
    • Journal Title

      耳鼻咽喉科免疫アレルギー 22/2

      Pages: 26-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 口蓋扁桃摘出術の適応2004

    • Author(s)
      藤原啓次, 山中 昇
    • Journal Title

      日本耳鼻咽喉科学会 専門医通信 79

      Pages: 24-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The guideline for tonsillectomy, the indication for tonsillectomy from the medical economy in Japan, United States and Germany.2004

    • Author(s)
      K Fujihara, M Hayashi, H Goto, M Hotomi, S Tamura, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of stomato-pharyngology 16(2)

      Pages: 151-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The indication for tonsillectomy.2004

    • Author(s)
      K Fujihara, N Yamanaka
    • Journal Title

      The communication in Japan otorhinolaryngology. 79

      Pages: 24-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The study for Heat Shock Protein in focal tonsillar infection (The third report)2004

    • Author(s)
      M Hayashi, K Fujihara, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of Immunology, Allergology in Otolaryngology 22(2)

      Pages: 26-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 扁桃摘出術のガイドライン 日米独 医療経済効果からみた扁桃摘出術の適応について2004

    • Author(s)
      藤原啓次, 林正樹, 後藤浩伸, 保冨宗城, 田村真司, 九鬼清典, 山中昇
    • Journal Title

      口腔・咽頭科 16/2

      Pages: 151-154

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 扁桃病巣感染症における熱ショック蛋白に関する研究(第2報)2003

    • Author(s)
      林 正樹, 藤原啓次, 九鬼清典, 山中 昇, 他
    • Journal Title

      耳鼻咽喉科免疫アレルギー 21/2

      Pages: 88-89

    • NAID

      10012081335

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] he pathogenesis of recurrent tonsillitis and the indication, effectiveness of tonsillectomy.2003

    • Author(s)
      K Fujihara, M Hayashi, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan society of stomato-pharyngology 15(3)

      Pages: 379-382

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The study for Heat Shock Protein in focal tonsillar infection (The second report)2003

    • Author(s)
      M Hayashi, K Fujihara, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of Immunology, Allergology in Otolaryngology 21(2)

      Pages: 88-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 習慣性扁桃炎の発症病態と扁桃摘出術の適応と有効性2003

    • Author(s)
      藤原啓次, 林正樹, 後藤浩伸, 保冨宗城, 田村真司, 九鬼清典, 山中昇
    • Journal Title

      口腔・咽頭科 15/3

      Pages: 379-382

    • NAID

      10011192605

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The study for Heat Shock Protein in focal tonsillar infection2002

    • Author(s)
      M Hayashi, K Fujihara, K Kuki, N Yamanaka
    • Journal Title

      Immunology allergy in Otorhinolaryngology 20(2)

      Pages: 96-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] IgA腎症患者における扁桃リンパ球のサイトカイン産生2002

    • Author(s)
      後藤浩伸, 九鬼清典, 林正樹, 藤原啓次, 山中昇
    • Journal Title

      耳鼻咽喉科免疫アレルギー 21/2

      Pages: 98-99

    • NAID

      10012081352

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 扁桃,皮膚におけるHeat Shock Protein 72 (HSP72)の発現について2001

    • Author(s)
      林 正樹, 山本良一, 藤原啓次, 九鬼清典, 山中 昇
    • Journal Title

      耳鼻咽喉科免疫アレルギー 19巻2号

      Pages: 120-121

    • NAID

      10012991307

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Engraftment of tonsillar mononuclear cells in human skin/SCID mouse chimera-validation of a novel xenogeneic transplantation model for autoimmune diseases2001

    • Author(s)
      Yamanka N, Yamamoto Y, Kuki K
    • Journal Title

      Microbiol.Immunol. 45

      Pages: 507-514

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Is tonsillectomy effective for pustulosis palmaris et plantaris in contention of department of Dermatology in 2001.2001

    • Author(s)
      N Yamanaka, K Fujihara, Y Yamamoto, K Kuki.
    • Journal Title

      Japan Journal of Dermatology 111(12)

      Pages: 1801-1803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The appearance of Heat Shock Protein 72(HSP72) in the skin2001

    • Author(s)
      M Hayashi, K Fujihara, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of Immunology, Allergology in Otolaryngology 19(2)

      Pages: 120-121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The appearance of cytokeratin in the adenoid epithelia2000

    • Author(s)
      K Fujihara, M Hayashi, K Kuki, N Yamanaka
    • Journal Title

      Journal of Japan Society of Immunology, Allergology in Otolaryngology 18(2)

      Pages: 50-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Current topics on tonsils and mucosal barriers of upper airways2003

    • Author(s)
      N Yamanaka, K Kuki, K Fujiharaha, M Hotomi.
    • Total Pages
      305
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 林 正樹, 藤原啓次, 九鬼清典, 山中 昇: "扁桃病巣感染症における熱ショック蛋白に関する研究(第2報)"耳鼻咽喉科免疫アレルギー. 21巻2号. 88-89 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 藤原啓次, 林 正樹, 九鬼清典, 山中 昇: "習慣性扁桃炎の発症病態と扁桃摘出術の適応と有効性"口腔・咽頭科. 15巻3号. 379-382 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 林 正樹, 後藤浩伸, 藤原啓次, 九鬼清典, 山中 昇: "扁桃病巣感染症における熱ショック蛋白に関する研究"耳鼻咽喉科免疫アレルギー. 20巻2号. 96-98 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] N Yamanaka, K Kiki, K Fujiharaha, M Hotomi: "Current topics on tonsils and mucosal barriers of upper airways"Elsevier International Congress Series 1257. 305 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 林 正樹, 藤原啓次, 九鬼清典, 山中 昇: "扁桃、皮膚におけるHeat Shock Protein 72 (HSP72)の発現について"耳鼻咽喉科免疫アレルギー. 19/2. 120-121 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 後藤啓次, 林 正樹, 九鬼清典, 山中 昇: "アデノイド上皮におけるサイトケラチンの発現について 滲出性中耳炎合併群と非合併群における比較検討"耳鼻咽喉科免疫アレルギー. 18/2. 50-51 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] 後藤浩伸, 斎藤匡人, 九鬼清典, 山中 昇: "扁桃におけるco-stimulatory factorの検討(第二報)"耳鼻咽喉科免疫アレルギー. 18/2. 124-125 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 正樹, 藤原啓次, 九鬼清典, 山中 昇: "扁桃病巣感染症における熱ショック蛋白に関する研究"耳鼻咽喉科兔疫アレルギー. 20/2. 96-97 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 後藤浩伸, 斎藤匡人, 九鬼清典, 山中 昇: "口蓋扁桃の加齢による免疫応答の変化-co-stimulatory factorの観点から-"耳鼻咽喉科免疫アレルギー. 20/2. 90-91 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 藤原啓次, 島田 純, 山中昇: "エビデンスに基づいたエアロゾル療法 鼻副鼻腔検出菌とエアロゾル療法について"耳鼻咽喉科展望. 45/補1. 41-45 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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