| Project/Area Number |
14571655
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
HAYAKAWA Kouichi Akita University, School of Medicine, Assistant professor, 医学部, 助手 (80312700)
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| Co-Investigator(Kenkyū-buntansha) |
KONNNO Seiki Akita University, School of Medicine, assistant professor, 医学部, 助手 (00312710)
YAMAKI Kunihiko Akita University, School of Medicine, Associate professor, 医学部, 助教授 (20125751)
徐 魁イ 秋田大学, 医学部, 助手 (70272036)
櫻木 章三 秋田大学, 医学部, 教授 (80006767)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | VKH disease / T cell clone / yrosinase family protein / Inflammatory lesions / VKH disease / CSF |
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| Research Abstract |
We have established T cell clones(TCC) derived from the aqueous humors(AH) and cerebrospinal fluids(CSF) of Vogt-Koyanagi-Harada(VKH) disease patients. The TCCs were established with limiting dilution method after transforming the T cells with Herpesvirus Saimiri(HVS) C488. Four hundred forty four TCCs derived from AH of 3 patients and 958 TCCs from CSF of 7 patients were obtained. Among those TCCs, randomly selected 21 TCCS derived from the AH and 9 TCCs from the CSF of patient 1were challenged with tyrosinase and/or TRP1 peptide mixture. Ten out of 16 CD4 positive TCCs from the AH and 2 out of 6 CD4 positive TCCs from the CSF were reactive against tyrosinase or TRP1. The cytokine production profile showed that all except one of the CD4^+, CD8 TCCs reactive to tyrosinase or TRP were Th1 type. It was strongly suggested that the T cells reactive against tyrosinase and/or TRP1 play an important role to the induction and formation of the inflammatory lesions in VKH disease.
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