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Analysis of intraretinal neuro-glial interaction in glaucoma

Research Project

Project/Area Number 14571672
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKobe University

Principal Investigator

NEGI Akira  Kobe University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (00189359)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Makoto  Kobe University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (80273788)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsglaucoma / ocular hypertension / retinal ganglion cell / apoptosis / glia / グリア / GFAP / MAP1a
Research Abstract

1.In an attempt to elucidate the causal effect of diabetes on development of glaucomatous optic neuropathy, male Sprague-Dawley rats were made diabetic by streptozotocin injection, in which unilateral eyes were treated with episcleral vein cauterization to produce chronically elevated intraocular pressure (IOP). Terminal dUTP nick end-labeling (TUNEL) staining was performed on the flat-mount retina dissected at several time points of sacrifice. The number of TUNEL positive cells in the inner retina was significantly higher in diabetic retina with episcleral vein 'cauterization than in control retina with same operation. Thus, diabetes has an additive effect on neuronal apoptosis induced by glaucoma in rats.
2.In order to identify whether the pro-survival Akt pathway is activated in retina with chronically elevated IOP, immunohistochmeistry in the crysosection and in the whole mount retina as well as immunoblotting with retinal homogenates were conducted for rat retina with episcleral ve … More in cauterization. Akt phorphorylated at Ser 437 was specifically expressed in the retinal ganglion cells in the retina with cauterization, but not in the contralateral retina. Phosphorylated relative to total Akt was significantly increased in retina with cauterization. Thus, Akt activation may counteract against increased IOP stress in retina.
3.Finally, to test whether glial reactivity was altered in glaucomatous retina, immunostaining and immunoblotting for glial fibrially acidic protein (GFAP) was conducted in the same experimental glaucoma model as in 2. Astrocytes lost and Muller cells gained GFAP imunoreactivity as early as at 3 days after episcleral vein cauterization, which was partially reversed overtime but last up to 6 months. GFAP molecular mobility and acidity were also changed by the cauterization. Similar observations were also occurred in the contrallateral retina at 1 month and thereafter. Thus, epicsleral vein cauterization altered glial reactivity not only in the ipsilateral but also in the contralateral eyes in rats. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Makoto Nakamura: "Occult macular dystrophy accomapanying normal-tension glaucoma"American Journal of Ophthalmology. 135・5. 715-717 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu Kanamori: "Evaluation of the glaucomatous damage on retinal nerve fiber layer thickness measured by optical coherence tomography"American Journal of Ophthalmology. 135・4. 513-520 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu Kanamori: "Evaluation of the effect of aging on retinal nerve fiber layer thickness measured by optical coherence tomography"Ophthalmologica. 217・4. 273-278 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu Kanamori: "Diabetes has an additive effect on neural apoptosis in rat retina with chronically elevated intraocular pressure"Current Eye Research. 28・1. 47-54 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu Kanamori: "Goniosynechialysis with lens aspiration and posterior chamber intraocular lens implantation for glaucoma in spherophakia"Journal of Cataract and Refractive Surgery. 30・2. 513-516 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Makoto Nakamura: "Diabetes mellitus as a risk factor for glaucomatous optic neuropathy"Ophthalmologica. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Makoto, Nakamura: "Occult macular dystrophy accompanying normal-tension glaucoma"American Journal of Ophthalmology. 135-5. 715-717 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu, Kanamori: "Evaluation of the glaucomatous damage on retinal nerve fiber layer thickness measured by optical coherence tomography"American Journal of Ophthalmology. 135-4. 513-520 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu, Kanamori: "Evaluation of the effect of aging on retinal nerve fiber layer thickness measured by optical coherence tomography"Ophthalmologica. 217-4. 273-278 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu, Kanamori: "Diabetes has an additive effect on neural apoptosis in rat retina with chronically elevated intraocular pressure"Current Eye Research. 28-1. 47-54 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiyasu, Kanamori: "Goniosynechialysis with lens aspiration and posterior chamber intraocular lens implantation for glaucoma in spherophakia"Journal of Cataract and Refractive Surgery. 30-2. 513-516 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Makoto, Nakamura: "Diabetes mellitus as a risk factor for glaucomatous optic neuropathy"Ophthalmologica. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Makoto Nakamura: "Occult macular dystrophy accompanying normal-tension glaucoma"American Journal of Ophthalmology. 135・5. 715-717 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyasu Kanamori: "Evaluation of the glaucomatous damage on retinal nerve fiber layer thickness measured by optical coherence tomography"American Journal of Ophthalmology. 135・4. 513-520 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyasu Kanamori: "Evaluation of the effect of aging on retinal nerve fiber layer thickness measured by optical coherence tomography"Ophthalmologica. 217・4. 273-278 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyasu Kanamori: "Diabetes has an additive effect on neural apoptosis in rat retina with chronically elevated intraocular pressure"Current Eye Research. 28・1. 47-54 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Makoto Nakamura: "Diabetes mellitus as a risk factor for glaucomatous optic neuropathy"Ophthalmologica. In Press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Makoto Nakamura: "Occult macular dystrophy accompanying normal-tension glaucoma"American Journal of Ophthalmology. (In Press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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