| Project/Area Number |
14571679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kumamoto University |
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Principal Investigator |
ARIMURA Kazue Kumamoto University, Graduate School of Medical Sciences, Ophthalmogy and visual science, Assistant, 大学院・医学薬学研究部, 助手 (20343353)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Makiko Kumamoto University, Kumamoto University Hospital, Ophthalmogy, Assistant, 医学部附属病院, 助手 (10284770)
HIRATA Akira Kumamoto University, Kumamoto University Hospital, Ophthalmogy, Instructor, 医学部附属病院, 講師 (60295144)
木村 章 熊本大学, 医学部付属病院, 講師 (20284771)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | plasmin / MMP / retina / vitrectomy / diabetic retinopathy / vitreous / endogenous survival factor |
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| Research Abstract |
1.Plasmin Assisted Vitrectomy for Removal of Proliferative Membrane In Proliferative Diabetic Retinopathy
Purpose : To demonstrate the feasibility of autologous plasmin enzyme (APE) for treatment proliferative diabetic retinopathy with tractional retinal detachment. Design : Nonrandamized noncomparative interventional case series. Methods : Six eyes were treated with APE and were compared with thefellow eyes treated without APE in surgical time and incidence of complication. Results : Surgical time with APE was significantly reduced compared with that without APE (p = 0.03). latrogenic retinal tears were found in 2 out of 6 eyes control group. In contrast, there was no complication was found in APE group (0/6 vs. 3/6). During surgery, proliferative membrane was peeled only with vitreous cullter in APE group. No additional surgical techniques including membrane delamination or segmentation were needed. There was no significant difference in visual outcome within two group. Conclusion : R
… More
esults suggests that APE may be beneficial in the surgical treatment o proliferative membrahe of proliferative diabetic retinopathy.
2.Intravitreal plasmin Injection activates endogenous matrix metalloproteinase-2 in rabbit and human vitreous
Purpose : To investigate the effect of exogenous plasmin administration on the activity of endogenous matrix metalloproteinase-2 (MMP-2) in the rabbit and human vitreous. Methods : Plasmin purified from human serum (0.05, 0.25, and 0.5 IU) was injected into the rabbit eyes. Vitreous sample was collected 15 min after injection. In the same manner, the vitreous sample was collected at 15, 30, 120, and 360 min after 0.5 IU of plasmin injection. Human vitreous samples from 5 eyes of 5 patients with macular holes were also collected before and 15 min after plasmin injection. The active/pro MMP-2 ratio was calculated by the data of gelatin zymography from each samples. In addition, immunohistochemical analysis was performed to confirm the presence of MT1-MMP in rabbit eye. Results : The active/pro MMP-2 ratio in the vitreous after 0.25 or 0.5 IU of plasmin injection were significantly higher than that of control (p < 0.05). In time course, there was a significant difference between 15 min after plasmin injection and control. In human samples, the active/pro MMP-2 ratio after plasmin injection was significantly higher than the ratio before plasmin injection. Immunohistochemical study revealed the presence of MT1-MMP in inner retina. Conclusions : The present study indicated that endogenous MMP-2 in the vitreous was significantly activated by the administration of exogenous plasmin. Activation of endogenous MMP-2 may be induced by exogenous plasmin. Less
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