• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Application of regenerative medicine using stem cell system to pediatric surgical diseases

Research Project

Project/Area Number 14571698
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionUniversity of Tsukuba

Principal Investigator

KOMURA Hiroaki  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 講師 (80296128)

Co-Investigator(Kenkyū-buntansha) KANEKO Michio  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Professor, 大学院・人間総合科学研究科, 教授 (60152807)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsRegenerative medicine / Urinary bladder / Esophagus / Ischemic intestine / Seem Cell / Bone marrow / Scaffold / Child / 再生 / 羊膜 / 横隔膜 / 腸管
Research Abstract

Tissue-engineered urinary bladder wall using PLGA mesh-collagen hybrid scaffolds.
We developed 2 hybrid scaffolds by combining PLGA mesh for mechanical strength with collagen sponge or gel suitable for cell seeding and construct a tissue-engineered urinary bladder wall utilizing these hybrid scaffolds. The urothelial layer was generated 3 dimensionally by culturing urothelial cells with PLGA mesh and collagen sponge. The smooth muscle layer was constructed by culturing smooth muscle cells with PLGA mesh and collagen gel. And a novel tissue-engineered urinary bladder wall was constructed laminating the urothelial and smooth muscle layers.
Introduction of bone marrow derived mononuclear cells to the ischemic intestine
Intestinal ischemia-reperfusion model in rats was used. Superior mesenteric artery was clamped for 30 minutes and declamped. Then bone marrow derived mononuclear cells were introduced. Survival rate did not improved in comparison with control group.
In situ tissue engineering of the esophagus using gastric acellular matrix : Experimental evaluations in a rat model
Gastric acellular matrix(GAM) was prepared by means of mechanical and chemical treatments. Patch defects created in the abdominal esophagus were replaced by GAM patch. Each rat was sacrificed 2 weeks to six months after surgery. The specimen was examined macroscopically as well as microscopically. GAM patch graft provided good mucosal regeneration of the esophageal without stenosis or ballooning. However, the muscle regeneration was not observed in the graft sites.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (20 results)

All 2004 2003 2002 Other

All Journal Article (11 results) Book (2 results) Publications (7 results)

  • [Journal Article] Tissue engineered urinary bladder wall using scaffold construction2004

    • Author(s)
      Komuro H et al.
    • Journal Title

      American Journal of Urology Review 2-6

      Pages: 289-292

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 再生医学の小児外科への応用-Tissue engineeringから幹細胞の利用まで2004

    • Author(s)
      小室広昭
    • Journal Title

      小児外科 36-11

      Pages: 1425-1429

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tissue-engineered urinary bladder wall using scaffold construction.2004

    • Author(s)
      Komuro H, Nakanishi Y, Chen G, Kaneko M, Tateishi T, Ushida T
    • Journal Title

      American Journal of Urology Review 2(6)

      Pages: 289-292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of regenerative medicine to the treatment of pediatric surgical diseases : From tissue engineering to stem cell therapy2004

    • Author(s)
      Komuro H
    • Journal Title

      Japanese Journal of Pediatric Surgery 36(11)

      Pages: 1425-1429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tissue-engineered urinary bladder wall using scaffold construction2004

    • Author(s)
      Komuro H et al.
    • Journal Title

      American Journal of Urology Review 2・6

      Pages: 289-292

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 再生医学の小児外科への応用-Tissue engineeringから幹細胞の利用まで2004

    • Author(s)
      小室広昭
    • Journal Title

      小児外科 36・11

      Pages: 1425-1429

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tissue-engineered urinary bladder wall using PLGA mesh-collagen hybrid scaffold : A comparison study of collagen sponge and gel as a scaffold2003

    • Author(s)
      Nakanishi Y, Chen G, Komuro H et al.
    • Journal Title

      Journal of Pediatric Surgery 38・12

      Pages: 1781-1784

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Tissue-engineered urinary bladder wall using PLGA mesh-collagen hybrid scaffold : A comparison study of collagen sponge and gel as a scaffold.2003

    • Author(s)
      Nakanishi Y, Chen G, Komuro H, Ushida T, Kaneko S, Tateishi T, Kaneko M
    • Journal Title

      Journal of Pediatric Surgery 38(12)

      Pages: 1781-1784

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of Collagen sponge scaffold to muscular defects of the esophagus2002

    • Author(s)
      Komuro H et al.
    • Journal Title

      Journal of Pediatric Surgery 37・10

      Pages: 1409-1413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglets.2002

    • Author(s)
      Komuro H, Nakamura T, Kaneko M, Nakanishi Y, Shimizu Y
    • Journal Title

      Journal of Pediatric Surgery 37(10)

      Pages: 1409-1413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of collagen sponge scaffold to muscular defects of the esophagus2002

    • Author(s)
      Komuro H et al.
    • Journal Title

      Journal of Pediatric Surgery 37・10

      Pages: 1409-1413

    • Related Report
      2004 Annual Research Report
  • [Book] 図解 再生医療工学2004

    • Author(s)
      小室広昭ら(分担)
    • Total Pages
      10
    • Publisher
      工業調査会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 図解再生医療工学2004

    • Author(s)
      小室広昭(分担)
    • Total Pages
      10
    • Publisher
      工業調査会
    • Related Report
      2004 Annual Research Report
  • [Publications] Yuka Nakanishi: "Tissue-engineered urinary bladder wall using PLGA mesh-collagen hybrid scaffolds : A comparison study of collagen sponge and gel as a scaffold"Journal of Pediatric Surgery. 38・12. 1781-1784 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小室 広昭: "PLGA-collagen hybrid scaffoldをもちいた膀胱壁の再構築"日本小児泌尿器学会雑誌. 12・1. 44 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小室 広昭: "PLGA-collagen hybrid scaffoldをもちいた対外での膀胱壁構築"日本小児外科学会雑誌. 39・3. 390 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中西 由佳: "PLGA-collagen hybrid scaffoldを用いた生体における膀胱組織再生"日本再生医療学会雑誌. 2・suppl. 143 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroaki Komuro: "Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglet"Journal of Pediatric Surgery. 37-10. 1409-1413 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小室 広昭: "Tissue engineeringによる体外への膀胱壁構築の試み"日本小児外科学会雑誌. 38・3. 417 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroaki Komuro: "Ex-vivo Construction of Urinary bladder wall using PLGA mesh Collage sponge hybrid Scaffold"Tissue Engineering. 8・6. 1176 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi