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Pulmonary Expression of Adrenomedullin in a Rat Model of Congenital Diaphragmatic Hernia

Research Project

Project/Area Number 14571702
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionOsaka University

Principal Investigator

KAMATA Shinkichi  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (40161202)

Co-Investigator(Kenkyū-buntansha) KANAGAWA Kenji  Department of Biochemistry, National Cardiovascular Center Research Institute, Director, 部長 (00112417)
USUI Noriaki  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30273626)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsadrenomedullin / lung development / congenital diaphragmatic hernia / nitrofen
Research Abstract

Background/Purpose: While the prognosis in congenital diaphragmatic hernia (CDII) with hypoplastic lung is still poor in spite of recent progress of the therapy, lung growth in the period of fetus and newborn has been thought to be more important. Adrenomedullin (AM), a novel vasodilating peptide, has also been found to play a role in the mechanisms of fetal lung differentiation and maturation. In this study, we examined pulmonary expression of adrenomedullin in a rat model of CDH. Methods: Congenital diaphmgmatic hernia was induced in timed-pregnant SD rats (term:22 days) after administration of 400 mg/kg of nitrofen in olive oil on day 9.5 of gestation and fetal lungs were harvested on day 16, 19, and2l. In control animals, the same doze of olive oil was given without nitrofen. Lung-to-body weight ratio and pulmonary AM were measured in nitrofen group and control group. Results: Lung-to-body weight ratio (%)was significantly lower in nitrofen group (1.24+/-0.13 at gestational day 16, 3.03+/-0.25 at gestational day 19, 1.85+/-0.26 at gestational day 21) compared with control group(1.63+/-0.30, 4.16+/-0.45,2.55+/-0.26). Pulmonary AM (fmol/mg wet tissue) was decreased significantly at gestational day 19 and 21 in nitrofen group (4.51+/-0.78 at gestational day 19, 4.95+/-1.95 at gestational day 21): compared with control group (1.35+/-0.36,3.2+/-1.17), while there is no significantly deference at gestational day 16 (0.44+/-0.10 vs 0.46+/-0.13). Conclusion: Increased pulmonary expression of adrenomedullin in a rat model of CDH in the late gestation suggest that AM can be one of growth factors of the lung.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Kamata S, Kamiyama M, Usui N, et al.: "Is adrenomedullin involved in the pathophysiology of persistent pulmonary hypertension of the newborn?"Pediatr Surg Int. 20. 24-26 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamata S, Kamiyama M, Usui N, Kitayama Y, Okuyama H, Kubota A, Kangawa K: "Is adrenomedullin involved in the pathophysiology of persistent pulmonary hypertension of hte newborn?"Pediatr Surg Int. 20. 24-26 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamata S, Kamiyama M, Usui N et al.: "Is adrenomedullin involved in the pathophysiology of persistent pulmonary hypertension of the newborn?"Pediatr Surg Int. 20(1). 24-26 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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