| Project/Area Number |
14571713
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
NAMBA Yuzaburo Okayama University, Graduate School of Medicine and Dentistry, Lecturer, 大学院・医歯学総合研究科, 講師 (00335605)
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| Co-Investigator(Kenkyū-buntansha) |
KOSHIMA Isao Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (60101804)
TSUTSUI Tetsuya Okayama University, University Hospital of Medicine and Dentistry, Assistant, 医学部・歯学部附属病院, 助手 (30346431)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Allotransplantation / Immune tolerance / Suppressor cell / Testis transfer |
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| Research Abstract |
The aim of this research is to introduce and maintain the immune tolerant system not with the help of immune suppressor drugs, but with immune regulatory cells which are introduced ex vivo. The first step of this research for clinical application is to develop a rat organ transfer model. All this rat organ transfer should be performed under magnification, so we had to get used to do super-microsurgical operation. We developed some models such as free flap transfer model and leg re-plantation model. In the former model, we once elevate a lower abdominal flap with superficial epigastric vessels (0.5mm) and then re-anastomose the pedicle to original vessels. In the latter model, we cut off the rat's thigh completely and then re-anastomose the femoral vessels and nerves and coaptate bone and muscles. We could succeed almost perfectly in these rat models. We had to give up the inbred rat propagation because reproductive abilities reduced gradually. So we could not get lymphocytes which own immune inhibitory abilities. We develop a rat testicular transfer model in a new turn. We also investigate the transferred testicular endocrine secretion and the ability of sperm formation. Practically, at first we get testis, epididymis and sperm cord from donor mature male LEW rat and make testectomy of recipient mature male LEW rat. Secondly, we anastomose the donor testicular vessels to the recipient superficial epigastric vessels. And then we pull the recipient sperm cord through abdominal muscles from abdominal cavity and anastomose it to the donor sperm cord in end to end style. The transferred donor testis is detained in recipient groin area. As a result we recognized secretion of testosterone and sperm formation in the transferred testis. This new model of testicular transfer is minimum invasive, easy to perform and has not been reported.
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