| Project/Area Number |
14571716
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | OSAKA CITY UNIVERCITY |
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Principal Investigator |
MURAOKA Michinari Osaka City University, Graduate School of Medicine, Assistant professor, 大学院・医学研究科, 助教授 (40117982)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Mitsuyo Osaka City University, Graduate School of Medicine, lecturer, 大学院・医学研究科, 講師 (40122080)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | IGF-1 / gene therapy / adenovirus vector / Cre / IoxP system / tongue / regeneration / 舌再建 / 骨格筋再生 / アデノウイルスベクター / ラット舌切除モデル |
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| Research Abstract |
IGF-1 plays an important role in the development, maintenance and regeneration of skeletal muscle. The effects of IGF-1 on myogenic cells include stimulation of myoblast replication, myogenic differentiation and myotube hypertrophy. In muscle regeneration, a proliferation of muscle precursor cells, fusion into myotubes, and reinnervation are involved. In this process, IGF-1 produced in satellite cells acts as a powerful stimulation for proliferation and differentiation of muscle precursor cells. We scrutinized the applicability and efficacy of adenovirus vector expressing human insulin-like growth factor-1 (hIGF-1) in gene transfer into cultured L6 cells and also cutting tongue muscle. Adenovirus vector-mediated hIGF-1 gene transfer showed the enhancement of myogenic differentiation and hypertrophy of L6 cells.The introduction of hIGF-1 into the cutting tongue muscle showed a significant increase in regenerating, and also an increase in the total number of myofibers, in comparison to that seen in the control muscle. These expressions demonstrate that hIGF-1 gene transfer using adenovirus vector may therefore have great potential in the treatment of neuromuscular disorders.
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