| Project/Area Number |
14571718
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
YANAI Akira Juntendo University, School of Medicine, Professor, 医学部, 教授 (80114495)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Hajime Juntendo University, School of Medicine, Professor, 医学部, 教授 (70167229)
SEKI Tatsunori Juntendo University School of Medicine, School of Medicine, Instructor, 医学部, 講師 (20175417)
TAKIGAWA Junko Juntendo University School of Medicine, School of Medicine, Assistant, 医学部, 助手 (90053339)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | End to side neurorrhapy / Nerve regeneration / Nerve graft / Neurorrhapy / Side to end neurorrhapy / Peripheral nerve / Peipheral nerve surgery / Double innervation / 末梢神経端側縫合 / 末梢神経端側吻合 |
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| Research Abstract |
The mechanism of nerve regeneration in this procedure is still not clear. To elucidate it, we designed complete non-injurious end-to-side coaptation methods in rat. The regenerated axons at the coaptatoin site were observed directly using fluorescent dye(DiI) as a neural tracer. In a complete non-injury models, the nerve regenerated electrophysically and histologically. Both right and left flexor muscles of the upper arm contracted simultaneously by the indirect electric stimulation on the grafted nerve. It suggested that the regenerated nerve connected with the recipient nerve by collateral sprouting. DiI stained the regenerated nerve beyond the coaptation site even in a non-injury model. The regenerated nerve was observed from the whole section of the coaptation site and we could also observed the regenerated nerve sprouted from the recipient nerve collaterally. From these results, we conclude that even in the end-to-side neurorrhaphy the nerve regenerated by the collateral sprouting from the donor nerve.
We tried again the end-to-side neurorrhaphy in the recipient nerve for clinical application of avoidance the cutting off the original nerve.We examined several-side at the distal portion of end-to-side neurorraphy. From these result, we confirmed that regeneration is possible at the distal portion of end-to-side neurorraphy.
At the clinical operation, we often use the cross face nerve graft and the cross over operation by anastomosis at the hypoglossal-facial nerve, at the same time. In the cases, two nerves regenerate toward to one target musle. So, we investigated the mechanism of regeneration at such cases.
From these results, we conclude the possibility of the double nerve direction term, in that cases.It seems necessary to examine more detail by changing time of regeneration term from the original nerve root.
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