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Possible role of immunocompetent cells and the expression of Hsp25 in the process of pulpal regeneration

Research Project

Project/Area Number 14571727
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

OHSHIMA Hayato  NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (70251824)

Co-Investigator(Kenkyū-buntansha) SATO Takuichi  Tohoku University, Graduate School of Dentistry, Lecturer, 大学院・歯学研究科, 講師 (10303132)
FUJII Noritaka  NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (90313527)
NAKAKURA Kuniko (OHSHIMA Kuniko)  NIIGATA UNIVERSITY, Medical and Dental Hospital, Lecturer, 医歯学総合病院, 講師 (80213693)
MAEDA Takeyasu  NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40183941)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsDental Pulp / Regeneration / Low Molecular Weight Heat Shock Protein / Antigen-presenting cells / Cavity Preparation / Replantation / Odontoblasts / Immunohistochemistry / 低分子熱ショック蛋白Hsp25
Research Abstract

The purpose of the present study was to clarify the relationship between the chronological changes of immunocompetent cells and the expression of heat shock protein(Hsp) 25 in the process of pulpal regeneration after tooth injury in rat molars by immunocytochemistry for Hsp 25 and class II major histocompatibility complex(MHC) antigen. An intense Hsp 25-immunoreactivity was found in the differentiated odontoblasts. Both cavity preparation and tooth replantation caused the degeneration of the odontoblast layer to result in the loss of Hsp 25-immunoreactions in the suffered dental pulp at the early stages after tooth injury. Numerous class II MHC-positive cells appeared along the pulp-dentin border and extend their cell processes into the dentinal tubules at 12-24 hours after cavity preparation and 3 days after tooth replantation. Newly differentiated odontoblast-like cells with Hsp 25-immunoreactivity were arranged at the pulp-dentin border, and the class II MHC-positive cells retreated … More towards the subodontoblastic layer by postoperative 3-5 days after tooth injury. Thus, the common cellular events occur during pulpal regeneration following two different experimental injuries. These findings indicate that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the degeneration/regeneration process of odontoblasts and that the temporal appearance of the class II MHC-positive cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. In the case of laser ablation, on the other hand, distinct abscess formation consisting of polymorphonuclear leukocytes was found in the dental pulp by 3-5 days postoperation. The penetration of masses of oral bacteria was recognizable in the dentinal tubules beneath the prepared cavity. These findings indicate that cavity preparation by laser ablation induces remarkable inflammation by continuous bacterial infections via dentinal tubules. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Ohshima, H. 他1名: "Functional significance of heat shock protein (Hsp) 25 during pulpal regeneration after cavity preparation and tooth replantation in rat molars."Proceeding, Program and Abstract of the 7^<th> International Seminar for JSPS-NRCT Core University Program. 1-5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, H. 他2名: "Expression of heat-shock protein 25 immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat molar."Connective Tissue Research. 43・2-3. 220-223 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakakura-Ohshima, K. 他3名: "Possible role of immunocompetent cells and the expression of heat shock protein-25 in the process of pulpal regeneration after tooth injury in rat molars"Journal of Electron Microscopy. 52・6. 581-591 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, H. 他5名: "Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells"Microscopy Research and Technique. 60・5. 483-490 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tamura, H. 他3名: "Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat"Journal of Periodontal Research. 38・1. 10-19 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, T. 他3名: "An immunocytochemical study of pulpal responses to cavity preparation by laser ablation in rat molars by using antibodies to heat shock protein (Hsp) 25 and class II MHC antigen"Cell and Tissue Research. 315・3. 311-319 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大島勇人(分担執筆): "パイロットアトラス 標本から学ぶ口腔の発生と組織"医歯薬出版株式会社. 209 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ohshima, K.Nakakura-Ohshima, T.Maeda: "Expression of heat-shock protein 25 immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat molar."Connect.Tissue Res.. 43(2-3). 220-223 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ohshima, T.Maeda, R.Maas, I.Satokata: "Functional significance of Msx2 gene during tooth development."Dentin/Pulp Complex, Proceeding of the International Conference on Dentin/Pulp Complex 2001, (Ishikawa, T., Takahashi, K., Maeda, T., Suda, H., Shimono, M. and Inoue, T. (Eds))(Quintessence Publishing Co.Ltd., Tokyo). 11-14 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ohshima, KNakakura-Ohshima: "Functional significance of heat shock protein(Hsp) 25 during pulpal regeneration after cavity preparation and tooth replantation in rat molars."Proceeding, Program and Abstract of the 7th International Seminar for JSPS-NRCT Core University Program. 1-5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ohshima, K.Nakakura-Ohshima, K.Takeuchi, M.Hoshino, Y.Takano, T.Maeda: "Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells."Microsc.Res.Tech.. 60(5). 483-490 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Fujii, H.Ohnishi, M.Shirakua, S.Nomura, H.Ohshima, T.Maeda: "Regeneration of nerve fibers in the peri-implant epithelium incident to implantation in the rat maxilla as demonstrated by immunocytochemistry for protein gene product 9.5(PGP 9.5) and calcitonin gene-related peptide(CGRP)."Clin.Oral Implants Res.. 14(2). 240-247 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ohshima, S.Kenmotsu, H.Harada: "Use of the term apical bud to refer to the apical end of the continuously growing tooth."Arch.Comp.Biol.Tooth Enamel. 8. 45-49 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Shirakura, N.Fujii, H.Ohnishi, Y.Taguchi, H.Ohshima, S.Nomura, T.Maeda: "Tissue response to titanium implantation in the rat maxilla, with special reference to the effects of surface conditions on bone formation."Clin.Oral Implants Res.. 14(6). 687-696 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakakura-Ohshima, J.Watanabe, S.Kenmotsu, H.Ohshima: "Possible role of immunocompetent cells and the expression of heat shock protein(Hsp) 25 in the process of pulpal regeneration after tooth injury in rat molars."J.Electron Microsc.. 52(6). 581-591 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Suzuki, S.Nomura, T.Maed, H.Ohshima: "An immunocytochemical study of pulpal responses to cavity preparation by laser ablation in rat molars by using antibodies to heat shock protein(Hsp) 25 and class H MHC antigen."Cell Tissue Res.. 315(3). 311-319 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Harada, H.Ohshima: "New perspectives on tooth development and dental stem cell niche."Arch.Histol.Cytol.. 67(1). 1-11 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura-Ohshima, K.他3名: "Possible role of immunocompetent cells and the expression of heat shock protein-25 in the process of pulpal regeneration after tooth injury in rat molars"Journal of Electron Microscopy. 52・6. 581-591 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohshima, H.他5名: "Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells"Microscopy Research and Technique. 60・5. 483-490 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohshima, H.他2名: "Use of the term apical bud to refer to the apical end of the continuously growing tooth"Archives of Comparative Biology of Tooth Enamel. 8. 45-49 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tamura, H.他3名: "Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat"Journal of Periodontal Research. 38・1. 10-19 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, T.他3名: "An immunocytochemical study of pulpal responses to cavity preparation by laser ablation in rat molars using antibodies to heat shock protein (Hsp) 25 and class II MHC antigen"Cell and Tissue Research. 315・3. 311-319 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harada, H.他1名: "New perspectives on tooth development and dental stem cell niche"Archives of Histology and Cytology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大島勇人(分担執筆): "パイロットアトラス 標本から学ぶ口腔の発生と組織"医歯薬出版株式会社. 209 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohshima, H.: "Functional significance of heat shock protein (Hsp) 25 during pulpal regeneration after cavity preparation and tooth replantation in rat molars"Proceeding, Program and Abstract of the 7^<th> International Seminar for JSPS-NRCT Core University Program. 1-5 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohshima, H.: "Expression of heat-shock protein 25 immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat"Connect. Tissue Res.. 43・2-3. 220-223 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohshima, H.: "Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells"Microsc. Res. Tech.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohshima, H.: "Functional significance of Msx2 gene during tooth development"Dentin/Pulp Complex, Proceeding of the International Conference on Dentin/Pulp Complex 2001. 11-14 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohshima, H: "Use of the term apical bud to refer to the apical end of the continuously growing tooth"Arch. Comp. Biol. Tooth Enamel. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, H.: "Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat"J. Periodontal Res.. 38・1. 10-19 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大島勇人: "パイロットアトラス 標本から学ぶ口腔の発生と組織"医歯薬出版株式会社(印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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