| Project/Area Number |
14571729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
IKEGAME Mika NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Assistant Professor, 大学院・医歯学総合研究科, 助手 (70282986)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASHIMA Hiroyuki NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40169719)
EJIRI Sadakazu NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (40160361)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Stress, Mechanical / Osteoblasts / Cytoskeleton / Adhesion complex / Cell cortex / Ultrastructure |
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| Research Abstract |
Tensile stress(TS) has been shown, to stimulate osteogenesis in the cranial suture of mouse calvaria. In order to clarify the role of the adhesion complex and cytoskeleton in this mechanism, the morphological changes in such stressed suture cells was investigated.
TS was applied to the cranial suture of neonatal mouse calvaria in culture. Some were stained with Alexa phalloidin and their actin fibers observed with con focal laser scanning microscopy, with others being observed with TEM. The results show that in the osteogenic layers of the cranial suture, TS causes actin-fiber morphological change, principally in the osteoblasts located at the bone edge, as well as in the cells located in the center of the suture.
Furthermore, we previously demonstrated that TS induced protein production, as well as the gene expression of α-adaptin C following the initiation of loading. In situ hybridization and immunohistochemical analysis revealed that the induction of α-adaptin C mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes TS-induced osteoblast differentiation. Consistent with this result, TS significantly increased the number of coated pits and coated vesicles in the undifferentiated fibroblastic cells but not in the osteoblastic cells around calvarial bones. These results, taken together, suggest that TS accelerates osteoblast differentiation and osteogenesis, possibly through the induction of the α-adaptin C expression and consequent activation of receptor-mediated endocytosis.
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