• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Osteoclast Differentiation initiated at late-stage of mitosisof progenitor cells : Molecular mechanism of divergence from cell cycle

Research Project

Project/Area Number 14571738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

KUKITA Toshio  Kyushu University, Facult.Dental Science, Associate Professor, 歯学研究院, 助教授 (70150464)

Co-Investigator(Kenkyū-buntansha) NAGATA Kengo  Kyushu University, Facult.Dental Science, Research Associate, 歯学研究院, 助手 (90189134)
KUKITA Akiko  Saga Medical School, Associate Professor, 医学部, 助教授 (30153266)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsosteoclast / differentiation / proliferation of precursors / M-phase / cell surface antigen / affinity chromatography / mass-spectrometry / RAW-D cells / 銀染色
Research Abstract

Osteoclasts are multinucleated giant cells and it has been elucidated that mononuclear osteoclast precursors fuse each other to form multinucleated cells. Under the bone microenvironment composed of RANKL and other factors, osteoclastoprogenitors proliferate to differentiate into real osteoclasts, however, it has been ambiguous concerning the exact point of divergence into osteoclast-lineage in cell cycle. Previously we found the osteoclast-specific membrane molecule Kati-antigen expressed on rat osteoclasts. When we examined initiation of osteoclastogenesis using anti-Kati-antigen monoclonal antibody (mAb Kat1) as the tracer of osteoclastogenesis, we found an interesting observation in which Kati-antigen was expressed only in one daughter cells of dividing cells at the postmitotic phase of osteoclast progenitors. Mab Kati also reacts with rat astrocytic cell line RCR-1 cells. If the cell cycle of RCR-1 cells was synchronized to mitosis by treatment with colcemid, numerous RCR-1 cells in mitotic phase expressed Kati-antigen. Morphological analysis revealed that Kati-antigen was expressed only in one daughter cells of dividing cells at the postmitotic phase of RCR-1 cells in a similar pattern as osteoclast progenitors. As it was suggested that Kati-antigen is the receptor molecule determining the differentiation fate of the progenitors, we focused on elucidating the entity of this antigen and obtained data which suggest, that Kat-1 antigen was a related molecule with galectin 3. To separate postmitotic osteoclast progenitors into Kat1-positve cells and Kat1-negative cells was quite difficult in terms of experimental technology, and therefore we could not compare mRNA expression profiles among these two cell fractions. Now we are trying to construct M-phase specific cDNA library by use of RAW-D cells synchronized to M-phase. This study would enable an establishment of novel strategy of regulating bone resorption through targeting osteoclast progenitors in M-phase.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] K.Toh, T.Kukita et al.: "A possible involvement of MIP-1α in the recruitment of osteoclast progenitors to the distal tibia in rats with adjuvant-induced arthritis."Lab.Invest.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Z.Wu, K.Toh, K.Nagata, T.Kukita et al.: "Effect of the resection of the sciatic nerve on the Th1/Th2 balance in the synovia of the ankle joint of adjuvant arthritic rats."Histochem.Cell Biol.. 121. 141-147 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Watanabe, T.Kukita, A.Kukita et al.: "Direct stimulation of osteoclastogenesis by MIP-1α:Evidence obtained from studies using RAW264 cell clone highly responsive to RANKL."J.Endocrinol.. 180. 193-201 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kukita.A.Akiko, Licin Xu et al.: "Extremely high expression of β-actin mRNA in osteoclasts resorbing alveolar bone located at the distal area of the developing molar tooth germ in new born rats."Journal of Electron Microsc(Tokyo). 52. 545-550 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.M.Rahman, A.Kukita, T.Kukita et al.: "Two histon deacetylase inhibitor, trichostatin A and sodium butyrate, suppress differentiation of osteoclasts but not into macrophages."Blood. 101. 3451-3459 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Toh, T.Kukita et al.: "A possible involvement of MIP-la in the recruitment of osteoclast progenitors to the distal, tibia in rats with adjuvant-induced arthritis"Lab.Invest.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Z.Wu, K.Toh, K.Nagata, T.Kukita et al.: "Effect of the resection of the sciatic nerve on the Th1/Th2 balance in the synovia of the ankle joint of adjuvant arthritic rats."Histochem.Cell Biol. 121. 141-147 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Watanabe, T.Kukita, A.Kukita et al.: "Direct stimulation of osteoclastogenesis by MIP-1α : Evidence obtained from studies using RAW264 cell clone highly responsive to RANKL"J.Endocrinol. 180. 193-201 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kukita, A.Akiko, Licin Xu et al.: "Extremely high expression of β-actin mRNA in osteoclasts resorbing alveolar bone located at the distal area of the developing molar tooth germ in new born rats"Journal of Electron Microsc (Tokyo). 52. 545-550 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.M.Rahman, A.Kukita, T.Kukita et al.: "Two histon deacetylase inhibitor, trichostatin A and sodium butyrate, suppress differentiation of osteoclasts but not into macrophages"Blood. 101. 3451-3459 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wu Z, Toh K, Nagata K, Kukita T, Iijima T: "Effect of the resection of the sciatic nerve on the Th1/Th2 balance in the synovia of the ankle joint of adjuvant arthritic rats."Histochem Cell Biol. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe T, Kukita T, Kukita A et al.: "Direct stimulation of osteoclastogenesis by MIP-1sα : evidence obtained from studies using RAW264 cell clone highly responsive to RANKL."J Endocrinology. 180. 193-201 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久木田 敏夫, 久木田 明子: "破骨細胞機能の膜表面受容体を介した制御 特集「骨の細胞と形態機能」"CLINICAL CALCIUM(医薬ジャーナル社). 13・4. 444-448 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Rhaman M, Kukita A, Kukita T et al.: "Two histon deacetylase inhibitor, trichostatin A and sodium butylate, suppress differentiation of osteoclasts but not into macrophages."Blood. 101・9. 3451-3459 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uji A, Matsuda M, Kukita T, et al.: "Molecules interacting with PRIP-2, a novel Ins(1,4,5)P3 binding protein type 2 -Comparison with PRIP-1-"Life Science. 72. 443-453 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakai H, Jingushi S, Shuto T, Urabe K, Okazaki K, Kukita T, et al.: "Fibroblasts from the inner granulation tissue of the pseudocapsule in hips at revision arthroplasty induce osteoclast differentiation, as do stromal cells."Ann Rheum Dis. 61・2. 103-109 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久木田 敏夫, 久木田 明子: "破骨細胞機能の膜表面受容体を介した制御 特集「骨の細胞と形態機能」"CLINICAL CALCIUM (医薬ジャーナル社). (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 久木田 敏夫, 野見山 尚之: "破骨細胞分化とケモカイン 特集II ケモカインに関する最近の進歩"臨床免疫 (科学評論社). (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Rahman M., Kukita A, Kukita T et al.: "Two histon deacetylase inhibitor, trichostatin A and sodium butyrate, suppress differentiation of osteoclasts but not into macrophages"Blood. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Uji A, Matsuda M, Kukita T et al.: "Molecules interacting with PRIP-2, a novel Ins(1,4,5)P3 binding protein type 2 -Comparison with PRIP-1-"Life Science. 72. 443-453 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai H, Jingushi S, Shuto T et al.: "Fibroblasts from the inner granulation tissue of the pseudocapsule in hips at revision arthroplasty induce osteoclast differentiation, as do stromal cells"Ann.Rheum.Dis.. 61. 103-109 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi