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Cell biology of taste buds and analysis of taste transduction mechanisms

Research Project

Project/Area Number 14571740
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

TOYOSHIMA Kuniaki  Kyushu Dental College, Professor, 歯学部, 教授 (10112559)

Co-Investigator(Kenkyū-buntansha) SETA Yuji  Kyushu Dental College, Associate Professor, 歯学部, 助教授 (90291616)
TOYONO Takashi  Kyushu Dental College, Assistant Professor, 歯学部, 助手 (10311929)
KATAOKA Shinji  Kyushu Dental College, Assistant Professor, 歯学部, 助手 (80364149)
原田 英光  大阪大学, 歯学部, 助教授 (70271210)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsATP / ATP receptors / P2X3 / P2Y1 / RT-PCR / immunohistochemistry / in situ hybridization / IP3R3 / 味蕾 / 味覚 / うま味 / P2Xs / P2Ys / mGluR4 / ガストデューシン
Research Abstract

Extracellular nucleotides such as ATP are the signaling molecules which bind to membrane receptors (P2X ligand-gated ion channels and G-protein coupled P2Y families). In the gustatory system, P2X receptors (P2X_2 and P2X_3) are expressed exclusively in nerve fibers innervating taste buds. Also, P2Y receptors are appeared to play some roles in taste signal transductions in taste bud cells on the basis of the physiological studies. However, the expression patterns of P2Y receptor subtypes have not been revealed. In this study, the expression patterns of P2Y_1 receptor subtypes were examined by using RT PCR, immunohistochemistry and in situ hybridization in rat gustatory papillae. These analyses showed that P2Y_1 receptor was expressed in taste bud cells. Furthermore, we found that P2Y_1-expressed cells coexpressed both IP3R_3 and SNAP-25 by double immunolabeling. These results suggest that ATP may activate P2Y_1 receptors resulting in Ca^<2+> release from internal stores via IP3R3. Since all SNAP-25 immunoreactive taste bud cells coexpressed P2Y_1 immunoreactive, it is suggested that P2Y_1 expressing cells may make synapses with afferent nerve fibers. In addition to P2Y_1 receptors, P2Y_4 receptors were expressed a subset of taste bud cells. We concluded that both P2Y and P2X receptors appear to be important regulators of taste bud functions.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (34 results)

All 2004 2003 2002 Other

All Journal Article (22 results) Book (2 results) Publications (10 results)

  • [Journal Article] 味覚受容体2004

    • Author(s)
      豊野 孝 他(4名中4番目)
    • Journal Title

      顕微鏡 39(3)

      Pages: 168-171

    • NAID

      10014163746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of P2Y1 receptors in rat taste buds.2004

    • Author(s)
      Kataoka, S., et al.(4名中4番目)
    • Journal Title

      Histochemistry and Cell Biology 121

      Pages: 419-426

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of dental epithelial progenitor cells derived from cervical-loop2004

    • Author(s)
      Kawano S, et al.(11名中9番目)
    • Journal Title

      Journal of Dental Research 83(2)

      Pages: 129-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of phospholipase C-β4 in rat circumvallate papillae.2004

    • Author(s)
      Toyono, T., et al.(4名中4番目)
    • Journal Title

      Chemicak Senses 29(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulation of taste bud cell differentiation by Notch signaling pathway.2004

    • Author(s)
      Seta, Y., et al.(4名中4番目)
    • Journal Title

      Chemical Senses 29(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] In vitro differentiation of dental epithelial progenitor cells through epithelial-2004

    • Author(s)
      Morotomi T.et al.(8名中7番目)
    • Journal Title

      Archives of Oral Biology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of dental epithelial progenitor cells derived from cervical-loop Epithelium in a rat lower incisor.2004

    • Author(s)
      Kawano, S, Saito, M, Handa, K, Morotomi, T, Toyono, T, Seta, Y, Nakamura, N, Uchida, T, Toyoshima, K, Ohishi, M, Harada, H
    • Journal Title

      J.Dent.Res. 83(2)

      Pages: 129-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of P2Y1 receptors in rat taste buds.2004

    • Author(s)
      Kataoka, S, Toyono, T, Seta, Y, Ogura, T, Toyoshima, K
    • Journal Title

      Histochem.Cell Biol. 121(5)

      Pages: 419-426

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro differentiation of dental epithelial progenitor cells through epithelial-Mesenchymal interactions.2004

    • Author(s)
      Morotomi, T, Kawano, S, Toyono, T, Kitamura, C, Terashita, M, Uchida, T, Toyoshima, K, Harada, H
    • Journal Title

      Arch.Oral Biol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulation of taste bud cell differentiation by Notch signaling pathway.2004

    • Author(s)
      Seat, Y, Toyono, T, Kataoka, S, Toyoshima, K
    • Journal Title

      Chem.Senses 29(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of phospholopase C-β4 in rat circumvallate taste buds.2004

    • Author(s)
      Toyono, T, Kataoka, S, Seta, Y, Toyoshima, K
    • Journal Title

      Chem.Senses 29(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 味覚受容体2004

    • Author(s)
      豊野 孝, 他(4名中4番目)
    • Journal Title

      顕微鏡 39巻3号

      Pages: 168-171

    • NAID

      10014163746

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of P2Y1 receptors in rat taste buds.2004

    • Author(s)
      Kataoka, S., et al.(4名中4番目)
    • Journal Title

      Histochem.Cell Biol. 121

      Pages: 419-426

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 9.脊椎動物のMerkel細胞2004

    • Author(s)
      豊島 邦昭
    • Journal Title

      Merkel細胞・Merkel細胞癌-この謎多き細胞たち(小野 友道, 鈴木 啓之編集, 金原出版) (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor2004

    • Author(s)
      Kawano S, et al.(11名中9番目)
    • Journal Title

      J.Dent.Res. 83(2)

      Pages: 129-133

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of phospholipase C-β4 in rat circumvallate papillae.2004

    • Author(s)
      Toyono, T., et al.(4名中4番目)
    • Journal Title

      Chemical Senses 29(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of metabotropic glutamate receptor group I in rat gustatory papillae.2003

    • Author(s)
      Toyono, T, Seta, Y, Kataoka, S, Kawano, S, Shigemoto, R, Toyoshima, K
    • Journal Title

      Cell Tissue Res. 313(1)

      Pages: 29-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Villin is a possible marker of receptor cells in frog taste organs.2003

    • Author(s)
      Yoshie, S, Kumakura, M, Toyoshima, K
    • Journal Title

      Histochem.Cell Biol. 119(6)

      Pages: 447-450

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel gene, GliH1, with homology to the Gli zinc finger domain not required For mouse development.2002

    • Author(s)
      Nakashima, M, Tanese, M, Ito, M, Auerbach, W, Bai, C, Furukawa, T, Toyono, T, Akamine, A, Joyner, A.L.
    • Journal Title

      Mech.Develop. 119(1)

      Pages: 21-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FGF 10 maintains stem cell compartment in developing mouse incisors.2002

    • Author(s)
      Harada, H, Toyono, T, Toyoshima, K, Yamasak, N, Itoh, S, Kato, K, Sekine, K, Ohuchi, H
    • Journal Title

      Development 129(6)

      Pages: 1533-1541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epithelial stem cells in teeth.2002

    • Author(s)
      Harada, H, Mitsuyasu, T, Toyono, T, Toyoshima, K
    • Journal Title

      Odontology 90

      Pages: 1-6

    • NAID

      10015485827

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of the metabotropic glutamate receptor, mGluR4a, in the taste Hairs of taste buds in rat gustatory papillae.2002

    • Author(s)
      Toyono, T, Seta, Y, Kataoka, S, Harada, H, Morotomi, T, Kawano, S, Shigemoto, R, Toyoshima, K
    • Journal Title

      Arch.Histol.Cytol. 65(1)

      Pages: 91-96

    • NAID

      10012313968

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 新編 口腔組織学・発生学 第7章3.舌(脇田、山下、明坂、前田編集)2004

    • Author(s)
      豊島邦昭
    • Publisher
      医歯薬出版(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 新編 口腔組織学・発生学 第7章 3.舌(脇田、山下、明坂、前田編集)2004

    • Author(s)
      豊島 邦昭
    • Publisher
      医歯薬出版(印刷中)
    • Related Report
      2004 Annual Research Report
  • [Publications] Yoshie, S. 他: "Villin is a possible marker of receptor cells in frog taste organs."Histochem Cell Biol.. 119(6). 447-450 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Honda, E. 他: "Activation of muscarinic receptors in rat subfornical organ neurones."J.Neuroendocrinol.. 15(8). 770-777 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toyono, T. 他: "Expression of metabotropic glutamate receptor group I in rat gustatory papillae."Cell Tissue Res.. 313(1). 29-35 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seta, Y. 他: "Notch-associated gene expression in embryonic and adult taste papillae and taste buds suggests a role in taste cell lineage decisions."J.Comp.Neurol.. 464(1). 49-61 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ono, K. 他: "Evidence for the presence of nicotinic receptors on rat subfornical organ neurons."Auton.Neurosci.. 108(1-2). 87-90 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawano, S. 他: "Characterization of dental Epithelial Progenitor cells derived from cervical-loop epithelium in a rat Lower incisor."J Dent Res.. 83(2). 129-133 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 豊島邦昭: "分子生物学歯科小事典(分担執筆)"口腔保健協会. (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toyono, T., Seta, Y.et al.: "Expression of the metabotropic glutamate receptor, mGluR4a, in taste hairs of taste buds"Arch. Histol. Cytol.. 65. 91-96 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harada, H., Toyono, T.et al.: "FGF10 maintain stem cell compartment in -"Development. 129. 1533-1541 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harada, H., Mitsuyasu, T.et al.: "Epithelial stem cells in the teeth"Odontology. 90. 1-6 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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