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Functional analysis of DEC2 and its role in chondrogenic differentiation

Research Project

Project/Area Number 14571763
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

FUJIMOTO Katsumi  Hiroshima University, Graduate school of Biomedical Sciences, Research Assistant, 大学院・医歯薬学総合研究科, 助手 (40294566)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsDEC2 / bHLH transcription factor / MyoD / circadian rhythms / Clock / Bmal
Research Abstract

DEC2 is a basic helix-loop-helix (bHLH) protein related to hairy, Hes, and Hey, which are known to be involved in tissue development. Transcripts of DEC2 exhibit a striking circadian oscillation in the suprachiasmatic nucleus, suggesting that DEC2 functions as regulatory protein for the clockwork system
1.Functional analysis of DEC2 protein
DEC2 bound to CACGTG E-box sequences and repressed transcription from the CACGTG elements. In addition, DEC2 repressed MyoD-mediated transactivation by inhibiting of MyoD/E47 heterodimerization
2.Regulation of DEC2 gene expression
Clock/Bmal heterodimer, which acts as positive component in a core circadian loop, up-regulated expression of DEC2 mRNA. In contrast to the oscillation pattern seem in the kidneys of wild-type mice, DEC2 mRNA levels were very low and the circadian expressions were abolished in Clock/Clock mutant mice. Luciferase assays showed Clock/Bmal regulate DEC2 gene expression through interactions with CACGTG E-boxes in the DEC2 promoter. In addition, DEC2 repressed its own expression through binding to the E-boxes. Thus, transcription of DEC2 was positively regulated by Clock/Bmal and negatively regulated by DEC2 via the common CACGTG E-boxes

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Honma Sato: "Dec1 and Dec2 are regulators of the mammalian molecular clock"Nature. 419・6909. 841-844 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ketut Suardita: "Effects of overexpression of membrane-bound transferrin-like protein (MTf)on chondrogenic differentiation in Vitro"Journal of Biological Chemistry. 277. 48579-48586 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ming Shen: "Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages"Journal of Biological Chemistry. 277. 50112-50120 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigeru Ohno: "RGD-CAP(big-h3)exerts a negative regulatory function on mineralization in the human periodontal ligament"Journal of Dental Research. 81. 822-825 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryo Oda: "Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A"Journal of Cell Science. 116. 2029-2038 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeshi Kawamoto: "A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation"Biochem.Biophys.Res.Commun.. 313. 117-124 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matthew P.Butler: "Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice"J.Biol.Rhythms.. 19. 126-134 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuhide Noshiro: "Rhythmic expression of DEC1 and DEC2 in peripheral tissues : DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP"Genes Cells. 9. 317-329 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honma Sato: "Dec 1 and Dec2 are regulators of the mammalian molecular clock"Nature. 419. 841-844 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ketut Suardita: "Effects of overexpression of membrane-Bound transferrin-like protein (MTV on chondrogenic differentiation in Vitro"Journal of Biological Chemistry. 277. 48579-48586 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ming Shen: "Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages"Journal of Biological Chemistry. 277. 50112-50120 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shigeru Ohno: "RGD-CAP(big-h3) exerts a negative regulatory function on mineralization in the human periodontal ligament"Journal of Dental Research. 81. 822-825 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryo Oda: "Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A"Journal of Cell Science. 116. 2029-2038 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeshi Kawamoto: "A novel autofeedback loop of Dec 1 transcription involved in circadian rhythm regulation"Biochem.Biophys.Res.Commun.. 313. 117-124 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matthew P Butler: "Decl and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice"J.Biol.Rhythms. 19. 126-134 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuhide Noshiro: "Rhythmic expression of DEC1 and DEC2 in peripheral tissues : DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP"Genes Cells. 9. 317-329 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamoto Takeshi: "A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation"Biochem.Biophys.Res.Commun.. 313・1. 117-124 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oda Ryo: "Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A"J.Cell Sci.. 116. 2029-2038 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Honma Sato: "Dec1 and Dec2 are regulators of the mammalian molecular clock"Nature. 416・6909. 841-844 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suardita ketut: "Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in Vitro"J.Biol.Chem.. 277・50. 48579-48586 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shen.Ming: "Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages"J.Biol.Chem.. 277・51. 50112-50120 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ketut Suardita: "Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in Vitro"J. Biol. Chem.. 277(50). 48579-48586 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ming Shen: "Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages"J. Biol. Chem.. 277(51). 50112-50120 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato Honma: "Dec1 and Dec2 are regulators of the mammalian molecular clock"Nature. 419(6909). 841-844 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigeru Ohno: "RGD-CAP (betaig-h3) exerts a negative regulatory function on mineralization in the human periodontal ligament"J. Dent. Res.. 81(12). 822-825 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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