Relationship between Radiation Resistance and Mitochondrial Dysfunction in Human Squamous Cell Carcinoma cell Lines.

• Project/Area Number: 14571795
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 病態科学系歯学(含放射線系歯学)
• Research Institution: Kagoshima University
• Principal Investigator: AWANO Kazunori Kagoshima University, University Hospital, Faculty of Medicine and Dentistiy, Research Associate, 医学部・歯学部附属病院, 助手 (50108750)
• Co-Investigator(Kenkyū-buntansha): INDO Hiroko Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (00301391) ; HIRAKI Yoshiyuki Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (90264423) ; MAJIMA Hideyuki Kagoshima University, University Hospital, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60165701) ; TOMITA Kazuo Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (60347094)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥2,900,000 (Direct Cost: ¥2,900,000); Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Radiosensitivity / Radioresistance / Antitumor agent resistance / MnSOD

Research Abstract

Mechanisms for radiation and chemotherapeutic agent resistance were studied in this study.Among 12 Bleomycine resistant clones, 5 clones expressed highly resistance.Three of out the 5 clones, MnSOD activity revealed substantial increase compared to the partial clones.

Research Products

• [Publications] F.Hirai: "Mitochondrial Signal Lacking Manganese Superoxide Dismutase Failed to Prevent Cell Death by Reoxygenation following Hypoxia in a Human Pancreatic Cancer Cell Line, KP4."Antioxidant & Redox Signaling. 6(3). 523-535 (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.-C.Yen: "Increase of Lipid Peroxidation by Cisplatin in WI38 but not in SV40-transformed WI38 cells."J.Biochem.Mol.Toxicol.. 17(1). 1-8 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] S.Koike: "Relative biological effectiveness of 290 MeV/u carbon ions for the growth delay of a radioresistant murine fibrosarcoma."J.Radiat.Res.. 43(3). 247-225 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.J.Majima: "4-hydroxy-2-nonenal (4-HNE) staining by anti-HNE antibody."Methods Mol Biol. 196. 31-34 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hirai F, Motoori S, Kakinuma S, Tomita K, Indo HP, Kato H, Yamaguchi T, Yen H-C, St.Clair DK, Nagano T, Ozawa T, Saisho H, Majima HJ: "Mitochondrial Signal Lacking Manganese Superoxide Dismutase Failed to Prevent Cell Death by Reoxygenation following Hypoxia in a Human Pancreatic Cancer Cell Line, KP4."Antioxidant & Redox Signaling. 6(3). 523-535 (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yen H-C, Nien C-Y, Majima HJ, Lee C-P, Chen S-Y, Wei J-S: "Increase of Lipid Peroxidation by Cisplatin in W138 but not in sV40-transformed W138 cells."J.Biochem.Mol.Toxicol.. 17(1). 39-46 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Koike S, Ando K, Oohira C, Fukawa T, Lee R, Takai N, Monobe M, Furusawa Y, Aoki M, Yamada S, Shimizu W, Nojima K, Majima H: "Relative biological effectiveness of 290 MeV/u carbon ions for the growth delay of a radioresistant murine fibrosarcoma."J.Radiat.Res.. 43(3). 247-255 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Majima HJ, Nakanishi-Ueda T, Ozawa T: "4-hydroxy-2 -nonenal(4-HNE)staining by anti-HNE antibody."Methods Mol.Biol.. 196. 31-34 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] F.Hirai, K.Tomita, H.P.Indo, D.K.St.Clair, T.Ozawa, H.J.Majima et al.: "Mitochondrial Signal Lacking Manganese Superoxide Dismutase Failed to Prevent Cell Death by Reoxygenation following Hynoxia in a Human Pancreatic Cancer Cell Line, KP4."Antioxidant & Redox Signaling. (in press). (2004)
• [Publications] H.Kariyazono, K.Nakamura, H.J.Majima, R.Sakata, K.Yamada et al.: "Evaluation of anti-platelet aggregatory effects of aspirin, cilostazol and ramatroban on platelet-rich plasma and whole blood."Blood Coagulation & Fibrinolysis. (in press). (2004)
• [Publications] S.Kakinuma, A.Kubo, Y.Amasaki, K.Nojima, H.Majima et al.: "Loss of heterozygosity in heavy-ion-induced murine T-cell lymphomas."Biological Sciences in Space. 17・3. 187 (2003)
• [Publications] Yen H-C, Nien C-Y, Majima HJ, Lee C-P, Chen S-Y, Wei J-S: "Increase of Lipid Peroxidation by Cisplatin in WI38 but not in SV40-transformed WI138 cells"J.Biochem.Mol.Toxicol.. 17・1. 1-8 (2003)
• [Publications] Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T: "Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species"J Biol Chem. 278・5. 3170-3175 (2003)
• [Publications] Koike S, Ando K, Oohira C, Fukawa T, Lee R, Takai N, Monobe M, Furusawa Y, Aoki M, Yamada S, Shimizu W, Nojima K, Majima H.: "Relative biological effectiveness of 290 MeV/u carbon ions for the growth delay of a radioresistant murine fibrosarcoma"J Radiat Res. 43・3. 247-255 (2002)
• [Publications] Majima HJ, Nakanishi-Ueda T, Ozawa T.: "4-hydroxy-2-nonenal (4-HNE) staining by anti-HNE antibody"Methods Mol Biol. 196. 31-34 (2002)