| Project/Area Number |
14571806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Osaka Dental University |
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Principal Investigator |
MIYAMAE Masam Osaka dental univ., Dept of Dent., assist prof, 歯学部, 講師 (20298821)
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| Co-Investigator(Kenkyū-buntansha) |
DOMAE Naochika Osaka dental univ., Dept of Dent., professor, 歯学部, 教授 (60115889)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | periodontitis / atherosclerosis / ADMA / reactive oxygen species / phospholipase C / heart / preconditioning / guinea pig |
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| Research Abstract |
1.Is atherosclerosis in patient with periodontitis more severe than normal control?
The severity of atherosclerosis is assessed with the intima-media thickness (IMT) of the common carotid artery and pulse wave velocity (PWV). IMT of patients with periodontitis adjusted for age is slightly thicker than that of control group. The periodontitis group had an abnormally higher PWV compared with that of the control group.
This means that periodontitis is associated with an increased risk of atherosclerosis.
2.Is phospholipase C (PLC) involved in the cardioprotective effect of light ethanol?
Hearts were isolated from guinea pigs after drinking 2.5% ethanol for 16 weeks and were subjected to global ischemia and reperfusion using Langendorff apparatus. Hearts from animals drinking ethanol showed improved functional recovery and decreased myocyte damage when compared with controls. PLC blockade with U-73122 abolished the protection provided by ethanol consumption. These findings indicate that long-t
… More
erm light alcohol consumption reduces myocardial ischemia-reperfusion injury and that PLC is required for this cardioprotective effect of ethanol. This cardioprotective effect of long-term light alcohol consumption mimics PC and may, in part, account for the beneficial effect of light drinking on cardiac health.
3.Is PC mediated by reactive oxygen species produced during PC protocol?
PC preserves myocardial HEP and intracellular pHi during subsequent sustained ischemia. 31P-NMR data was correlated with myocyte ultrastructural changes using electron microscopy. Open chest pigs underwent 60 minutes of left anterior descending coronary artery occlusion. PC was elicited by a single episode of 5-minute occlusion and 5-minute reperfusion. The cell diffusible free radical scavenger, N-2-mercaptopropionyl glycine (MPG) or placebo saline were infused for 40 minutes starting 30 minutes before PC (PC+MPG group & PC group). Following PC, ATP and pHi were significantly preserved through 25 min of ischemia and phosphocreatine through 20 min of ischemia. This preservation of HEP and pHi was abolished by inhibiting ROS generation with MPG. HEP preservation with PC was associated with reduced ultrastructural damage as demonstrated by electron microscopy, including less myocyte swelling, myofibrillar disruption and nuclear chromatin margination.
These results suggest that the cardioprotective effect of light alcohol can be used for clinical application as a chronic ischemic preconditioning. Less
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