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Involvement of Periodontitis in ischemic heart disease : Potential Mechanisms for its Development

Research Project

Project/Area Number 14571806
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionOsaka Dental University

Principal Investigator

MIYAMAE Masam  Osaka dental univ., Dept of Dent., assist prof, 歯学部, 講師 (20298821)

Co-Investigator(Kenkyū-buntansha) DOMAE Naochika  Osaka dental univ., Dept of Dent., professor, 歯学部, 教授 (60115889)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsperiodontitis / atherosclerosis / ADMA / reactive oxygen species / phospholipase C / heart / preconditioning / guinea pig
Research Abstract

1.Is atherosclerosis in patient with periodontitis more severe than normal control?
The severity of atherosclerosis is assessed with the intima-media thickness (IMT) of the common carotid artery and pulse wave velocity (PWV). IMT of patients with periodontitis adjusted for age is slightly thicker than that of control group. The periodontitis group had an abnormally higher PWV compared with that of the control group.
This means that periodontitis is associated with an increased risk of atherosclerosis.
2.Is phospholipase C (PLC) involved in the cardioprotective effect of light ethanol?
Hearts were isolated from guinea pigs after drinking 2.5% ethanol for 16 weeks and were subjected to global ischemia and reperfusion using Langendorff apparatus. Hearts from animals drinking ethanol showed improved functional recovery and decreased myocyte damage when compared with controls. PLC blockade with U-73122 abolished the protection provided by ethanol consumption. These findings indicate that long-t … More erm light alcohol consumption reduces myocardial ischemia-reperfusion injury and that PLC is required for this cardioprotective effect of ethanol. This cardioprotective effect of long-term light alcohol consumption mimics PC and may, in part, account for the beneficial effect of light drinking on cardiac health.
3.Is PC mediated by reactive oxygen species produced during PC protocol?
PC preserves myocardial HEP and intracellular pHi during subsequent sustained ischemia. 31P-NMR data was correlated with myocyte ultrastructural changes using electron microscopy. Open chest pigs underwent 60 minutes of left anterior descending coronary artery occlusion. PC was elicited by a single episode of 5-minute occlusion and 5-minute reperfusion. The cell diffusible free radical scavenger, N-2-mercaptopropionyl glycine (MPG) or placebo saline were infused for 40 minutes starting 30 minutes before PC (PC+MPG group & PC group). Following PC, ATP and pHi were significantly preserved through 25 min of ischemia and phosphocreatine through 20 min of ischemia. This preservation of HEP and pHi was abolished by inhibiting ROS generation with MPG. HEP preservation with PC was associated with reduced ultrastructural damage as demonstrated by electron microscopy, including less myocyte swelling, myofibrillar disruption and nuclear chromatin margination.
These results suggest that the cardioprotective effect of light alcohol can be used for clinical application as a chronic ischemic preconditioning. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Masami Miyamae, et al.: "Oxygen radicals mediate ultrastructural and metabolic protection of preconditioning in vivo in pig hearts"Experimental & Clinical Cardiology. 7・4. 173-179 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masami Miyamae, et al.: "Phospholipase C activation is required for cardioprotection by ethanol consumption"Experimental & Clinical Cardiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyamae M, et al.: "Oxygen radicals mediate ultrastructural & metabolic protection of preconditioning in vivo in pig hearts."Exp Clin Cardiol. 7(4). 173-179 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyamae, et al.: "Phospholipase C activation is required for cardioprotection by ethanol consumption."Exp Clin Cardiol. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masami Miyamae et al.: "Oxygen radicals mediate ultrastructural and metabolic protection of preconditioning in vivo in pig hearts"Experimental & Clinical Cardiology. 7・4. 173-179 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masami Miyamae et al.: "Phospholipase C activation is required for cardioprotection by ethanol consumption"Experimental & Clinical Cardiology. in press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Masami Miyamae, et al.: "Oxygen free radicals mediate ultrastructural & metabolic protection of preconditioning in vivo in pig hearts"Canadian Experimental and Clinical Cardiology. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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