Inhibitory Effects of Antimicrobial Agent Macrolides on LPS-induced periodontal destruction in periapical region.

• Project/Area Number: 14571821
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Meikai University
• Principal Investigator: KIKUCHI Hirotaka Meikai University, School of dentistry, research associate, 歯学部, 助手 (70234193)
• Co-Investigator(Kenkyū-buntansha): SAKAGAMI Hirosbi Meikai University, School of dentistry, professor, 歯学部, 教授 (50138484)
• Project Period (FY): 2002 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: macrolides / apical periodontitis / endodontics

Research Abstract

Macrolides are well-known antimicrobial agent and has been reported to possess immunomodulating properties. The aim of the present study was to investigate the efficacy of macrolides as an inhibitor of lipopolysaccharide (LPS)-induced bone resorption via CD14. The mouse calbaria-derived bone cells was used in this study. LPS-induced expression of IL-1 alpha mRNA and production of IL-l, and Lipid A-induced production of the factor were all completely inhibited by treatment with erythromycin. Interestingly, LPS-induced formation of osteoclasts and stimulation of bone resorption in mouse calvarial cell culture were also inhibited by erythromycin treatment. These results suggest that erythromycin is a potent inhibitor of LPS-induced bone resorption in the cells. Based on these observations, Macrolides may prove to be an effective anti-inflammatory agent against LPS-stimulated periodontal destruction in periapical and periodontal diseases.

Research Products

• [Journal Article] マウスマクロファージ活性化に対する多血小板血漿及び洗浄血小板の効果 (2005)
  • Author(s): 須田朋代, 菊地寛高, 高山直士 他
  • Journal Title: 日本歯科保存学雑誌 48・1 Pages: 152-157
• [Journal Article] Cytotoxic activity strylchromones against human tumor cell lines. (2005)
  • Author(s): Momoi K, Sugita Y, Ishihara M, Satoh K, Kikuchi H et al.
  • Journal Title: In vivo 19・1 Pages: 157-164
• [Journal Article] Structure-activity relationships of a, b-unsaturated ketones as assessed by their cytotoxicity against oral tumor cells. (2004)
  • Author(s): Nakayachi T, Yasumoto E, Nakano K, Hashimoto K, Kikuchi H, Sakagami H
  • Journal Title: Anticancer Res 24 Pages: 737-742
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cytotoxicity and radical modulating activity of isoflavones and isofravones from Sophora species. (2004)
  • Author(s): Shirataki Y, Wakae M, Hashimoto K, Kikuchi H, Nishikawa H, Sakagami H
  • Journal Title: Anticancer Res 24 Pages: 1481-1488
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure -activity relationships of a, b-unsaturated ketones as assessed by their cytotoxicity against oral tumor cells (2004)
  • Author(s): Nakayachi T, Yasumoto E, Nakano K, Morshed SRM, Hashimoto K, Kikuchi H, Nishikawa H, Kawase M, Sakagami H
  • Journal Title: Anticancer Res 24 Pages: 737-742
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cytotoxicity and radical modulating activity of isoflavones and isoflavones from Sophora species (2004)
  • Author(s): Shirataki Y, Wakae M, Yamamoto Y, Hashimoto K, Satoh K, Ishihara M, Kikuchi H, Nishikawa H, Minagawa K, Motohashi N, Sakagami H
  • Journal Title: Anticancer Res 24 Pages: 1481-1488
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of apoptosis by b-diketones in human tumor cells. (2004)
  • Author(s): Nakano K, Nakayachi T, Yasumoto E, Kikuchi H et al.
  • Journal Title: Anticancer Res. 24 Pages: 711-718
• [Journal Article] Cytotoxic activity of deferiprone, maltol and rerated hydroxyketones against human tumor cell lines. (2004)
  • Author(s): Yasumoto E, Nakano K, Nakayachi T. Kikuchi H et al.
  • Journal Title: Anticancer Res. 24 Pages: 755-762
• [Journal Article] Cytotoxic activity of azulenes against human oral tumor cell lines. (2003)
  • Author(s): Wakabayashi H, Hashiba K, Yokoyama K, Hashimoto K, Kikuchi H, Nishikawa H, Kurihara T, Sakagami H
  • Journal Title: Anticancer Res 23 Pages: 3237-3241
  • NAID: 120005520608
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cytotoxic activity of tropolones against human oral tumor cell lines. (2003)
  • Author(s): Wakabayashi H, Yokoyama K, Hashiba K, Hashimoto K, Kikuchi H, Nishikawa H, Kurihara T, Sakagami H
  • Journal Title: Anticancer Res 23 Pages: 2385-2389
  • NAID: 120005520607
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cytotoxic activity of azulenes against human oral tumor cell lines (2003)
  • Author(s): Wakabayashi H, Hashiba K, Yokoyama K, Hashimoto K, Kikuchi H, Nishikawa H, Kurihara T, Satoh K, Shioda S, Saito S, Kusano S, Nakashima H, Motohashi N, Sakagami H
  • Journal Title: Anticancer Res 23 Pages: 3237-3241
  • NAID: 120005520608
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cytotoxic activity of tropolones against human oral tumor cell lines (2003)
  • Author(s): Wakabayashi H, Yokoyama K, Hashiba K, Hashimoto K, Kikuchi H, Nishikawa H, Kurihara T, Satoh K, Shioda S, Muto S, Terakubo S, Nakashima H, Motohashi N, Sakagami H
  • Journal Title: Anticancer Res 23 Pages: 2385-2389
  • NAID: 120005520607
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transforming growth factor-beta1 inhibits nitric oxide production of mouse osteoblastic cell line MC3T3-E1 through modulation of nuclear factor-kB activation (2002)
  • Author(s): Kikuchi, H., Furuya, K., Shimada, K., Nishikawa, H.
  • Journal Title: Meikai Univ Dent J 31 Pages: 204-209
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transforming growth factor-beta 1 inhibits nitric oxide production of mouse osteoblastic cell line MC3T3-E1 through modulation of nuclear factor k-B activation (2002)
  • Author(s): Kikuchi H, Furuya K, Shimada K, Kobayashi K, Suda T, Nakamura Y, Uchida A, Kotani Y, Sakata K, Nishikawa H
  • Journal Title: Meikai Univ Dent J 31 Pages: 204-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakayachi T, et al.: "Structure-activity relationships of a, b-unsaturated ketones as assessed by their cytotoxicity against oral tumor cells."Anticancer Research. 24(in press). (2004)
• [Publications] Shirataki Y et al.: "Cytotoxicity and radical modulating activity of isoflavones and isofravones from Sophora species."Anticancer Research. 24(in press). (2004)
• [Publications] Wakabayashi H, et al.: "Cytotoxic activity of azulenes against human oral tumor cell lines."Anticancer Research. 23. 3237-3241 (2003)
• [Publications] Wakabayashi H, et al.: "Cytotoxic activity of tropolones against human oral tumor cell lines."Anticancer Research. 23. 2385-2389 (2003)
• [Publications] Saori Mihara et al.: "Diverse biological activities of fermented pine seed shell extract"Anticancer Research. 22. 1569-1574 (2002)
• [Publications] Ikusuke Hatsukari et al.: "Partial purification of cytotoxic substances from moxa extract"Anticancer Research. 22. 2777-2782 (2002)
• [Publications] Hirotaka Kikuchi et al.: "Transforming growth factor-betal inhibits nitric oxide production of mouse osteoblastic cell line MC3T3-E1 through modulation of Nuclear Factor-kB"Meikai university dental journal. 31,(2)(in press). (2003)