| Project/Area Number |
14571831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
TSUCHIDA Mami Tsurumi University School of Dental Medicine, Dept of Peiodontics and Endodontics, Research Associate, 歯学部, 助手 (70207398)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Toshiko Tsurumi University School of Dental Medicine, Dept of Peiodontics and Endodontics, Assistant Professor, 歯学部, 助手 (10139610)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Endoscope / faiberscope / periapicallesion / root surface / calculus-like deposite / 根尖歯周病変 |
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| Research Abstract |
We have developed a newly designed endoscopic system (ES) with fiberscope (FS). The FS has made the visualization of the operation fields possible with introducing the FS into fistulae or periodontal pockets. The root surfaces of 27 cases involved in periapical lesions without periodontal pockets were observed with the FS through fistulae. The ES imaging showed calculus-like deposits on the root surfaces in 1 cases of 27 cases. Periapical surgery was performed in five cases showing calculus-like deposits on the root surfaces. During the operations, the samples were taken by scratching root surfaces with curette or scalar. A part of each sample was smeared on a slide grass and microscopic observation with Gram stain was performed. The rests of each sample were inoculated onto the CDC agar and incubated anaerobically.
The results were as follows :
1. ES imaging showed calculus-like deposits on the root surfaces in 21 cases out of 27 cases involved in periapical lesions with fistulae. 2. Gram-positive bacteria were observed as the non-structure tissues in the samples with Gram staining. 3. Gram (+) cocci, rods, and Gram (-) rods were isolated from the samples by anaerobic incubation.
These results suggest that the bacteria are concerned in calculus-like deposits on the root surfaces.
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