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ALVEOLAR BONE REGENERATION BY USING MESENCHYMAL STEM CELL AND PLATELET-RICH PLASMA

Research Project

Project/Area Number 14571846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionKYUSHU DENTAL COLLEGE (2003)
Hiroshima University (2002)

Principal Investigator

HOSOKAWA Ryuji  KYUSHU DENTAL COLLEGE, FUCALTY OF DENTISTRY, PROFESSOR, 歯学部, 教授 (60211546)

Co-Investigator(Kenkyū-buntansha) ANDO Hironobu  KYUSHU DENTAL COLLEGE, FUCALTY OF DENTISTRY, INSTRUCTOR, 歯学部, 助手 (40285466)
HATANO Kiyotoshi  KYUSHU DENTAL COLLEGE, FUCALTY OF DENTISTRY, INSTRUCTOR, 歯学部, 助手 (10326465)
山中 威典  広島大学, 歯学部附属病院, 助手 (20325202)
久保 隆靖  広島大学, 歯学部附属病院, 講師 (60240876)
赤川 安正  広島大学, 大学院・医歯薬学総合研究科, 教授 (00127599)
是竹 克紀  広島大学, 大学院・医歯薬学総合研究科, 助手 (50346508)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsmesenchymal stem cell / dental implants / PRP / 骨再生 / 顎骨再生 / 骨髄幹細胞 / ビーグル犬 / 歯科インプラント / FGF-2 / 組織形態計測 / 骨接触率
Research Abstract

The purpose of this study was to determine whether a combination of platelet-rich plasma (PRP) and mesenchymal stem cells (MSC) would accelerate new bone formation in a canine alveolar defect model. Three bone defects, measuring 3.75 x 7.0mm (diameter x deep) were prepared on each side of the edentulous areas in beagle dogs. The defects were then filled with one of the following four materials : 1) PRP+MSC, 2) PRP, 3) platelet-poor plasma (PPP), and 4) blood clot (control). New bone formation was assessed by histologic and morphometric methods 2 weeks after surgery. The results showed that a larger area of regenerated bone was seen in the defects that were filled with PRP+MSC and PRP. In the next experiment, two bone defects were created at the buccal side of each edentulous area. Titanium dental implants were then placed in each of two defects. Then the defects were filled with one of the following four materials : 1) blood clot (Controls), 2) PRP, 3) AB graft and 4) autogeneous bone (AB) graft with PRP. Ten weeks after surgery, new bone formation at the buccal defects was assessed using histological and histomorphometrical methods. Histologically, all defects were filled with newly formed bone with some variation. Although all exposed screws were covered with newly formed bone at the AB graft and the AB graft with PRP, exposed screws in the Control and PRP alone sites were not fully covered. The regenerated bone contact ratio of Controls, PRP, AB graft and AB graft with PRP were 24.1±20.8%, 59.7±8.8%, 39.2±3.5%, 41.1±13.5%, respectively Bone-implant contact was significantly higher (p<0.05) at the PRP site compared with the control site.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All 2004 2003 2002

All Journal Article (6 results) Book (1 results)

  • [Journal Article] インプラント周囲骨欠損に対する多血小板血漿の骨再生促進効果に関する実験的研究2003

    • Author(s)
      竹腰利英
    • Journal Title

      広大歯誌 35

      Pages: 33-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] AN EXPERIMENTAL STUDY ON ACCELERATION OF BONE REGENERATION WITH PLATELET-RICH PLASMA OF BONE DEFECT AROUND DENTAL IMPLANT2003

    • Author(s)
      TAKEKOSHI, T
    • Journal Title

      J Hiroshima Univ.Dent.Soc. 35

      Pages: 33-48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] PRP(plate-rich-plasma)の臨床応用2002

    • Author(s)
      細川隆司
    • Journal Title

      広大歯誌 34

      Pages: 111-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] PRP(plate-rich-plasma)による組織再生促進のメカニズム-臨床への展開と今後の課題-2002

    • Author(s)
      細川隆司 他
    • Journal Title

      歯界展望 100

      Pages: 1230-1243

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] CLINICAL APPLICATION OF PRP (PLATELET-RICH PLASMA)2002

    • Author(s)
      HOSOKAWA, R
    • Journal Title

      J Hiroshima Univ.Dent.Soc. 34

      Pages: 111-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] MECHANISM OF PRP (PLATELET-RICH PLASMA) ENHANSED TISSUE REGENERATION2002

    • Author(s)
      HOSOKAWA, R et al.
    • Journal Title

      The Dental Out-look 100

      Pages: 1230-1243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] インプラント補綴の最新エビデンス2004

    • Author(s)
      細川隆司, 渡邊文彦 編
    • Total Pages
      130
    • Publisher
      医歯薬出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary

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Published: 2002-04-01   Modified: 2016-04-21  

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