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The aim at efficiency of transplantation by regulation the defensin expression

Research Project

Project/Area Number 14571889
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MIZUKAWA Nobuyoshi  Okayama University, Hospitals, Lecturer, 医学部・歯学部附属病院, 講師 (00263608)

Co-Investigator(Kenkyū-buntansha) SUGAHARA Toshio  Okayama University Graduate School of Medicine and Dentistry, Department of Oral and Maxillofacial Reconstructive Surgery, Professor, 大学院・医歯学総合研究科, 教授 (10116048)
YAMAAI Tomoichiro  Okayama University Graduate School of Medicine and Dentistry, Department of Oral Function and Anatomy, Assistant, 大学院・医歯学総合研究科, 助手 (00158057)
中野 誠  岡山大学, 歯学部附属病院, 助手 (30335616)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordstransplantation / defensin / antimicrobialpeptide / HBD-2 / ヒスタミン / 口腔癌 / mRNA
Research Abstract

The biophiraxic barriers against external stimuli, mechanical trauma and microbiological pathogens consist of the oral epithelia and inflammatory cells. Defensins are cationic antimicrobial peptides, which are categorized into two types, human α-defensins (HNPs) and human β-defensins (HBDs). HBD-2, which is produced by the keratinocytes of the skin, is known to be strongly up-regulated by bacterial infections, lipopolysaccharides (LPS), interleukin (IL)-1 alpha, IL-1 beta and TNF-alpha.
Interestingly, HBD-2 expression is observed not only in the inflamed lesions associated with bacterial infection but also in other non-inflamed lesions such as carcinomas. Furthermore, so-called proliferation death of normal epidermal cells line (NHEK) was shown to be induced by HBD-2 generated by a squamous cell carcinoma cell line in vitro.
We identified that this effect of cellular proliferation death, is similar to the effect of low-dose irradiation on cells. This may in turn, help the multiplication of squamous cell carcinoma cells, because HBD-2 had no direct effect on the survival of squamous cell carcinoma cells. We suggested that up regulation of HBD-2 might have side effects, unrelated to defense mechanisms, which would lead to the death of keratinocytes adjacent to the SCC which indirectly assists in the multiplication of tumor cells.
Also, defensin was up-regulated in host side by transplantation as a surgical injury. We reported that defensin induced Histamine release from Mast Cells. Histamine was known to induce inflammation. And a High concentration of defensin was suggested to cause injury to neighboring normal cells. The purpose of our study was to regulate the defensin expression and obtain a favorable healing of grafts.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Mizukaw N., Sugahara T.: "Presence of human β defensin-2 in oral lichen planus and its histamine releasing effect"Asian J Oral Maxillofac Surg. 15. 112-117 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshimoto T, Yamaai T, Mizukawa N, Sugahara T.: "Different Expression Patterns of beta-Defensins in Human Squamous Cell Carcinomas"Anticancer Research. 23. 4629-4634 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawaki K., Yamaai T., Mizukawa N., Sugahara T.: "Mortality of human epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells"Anticancer Research.. 23. 79-84 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawaki K., Mizukawa N., Yamaai T., Sugahara T.: "High Concentration of β-Defensin-2 in Oral Squamous Cell Carcinoma"Anticancer Research. 22. 2103-2108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizukawa N, Sugahara T: "Histamine Release from Rat Mast Cells, Induced by Human α-Defensin-1 present in Jaw Cyst Fluid"Asian J Oral Maxillofac Surg. 14. 35-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Mizukawa N, Sugahara T: "Evaluation of Osteogenic /Chondrogenic cellular Proliferntiation in the Xenogenic Periosteal graft.."Ann Plast Surg. 48. 539-545 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Mizukawa, K.Sawaki, M.Kamio, J.Fukunaga, T.Ueno, S.Takagi, T.Sugahara: "Histamine release from rat mast cells induced by human alpha-defensin-1 present in jaw cyst fluid."Asian J Oral Maxillofac Surg. 14. 35-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ueno, T.Kagawa, J.Fukunaga, N.Mizukawa, T.Sugahara, T.Yamamoto: "Evaluation of Osteogenic/Chondrogenic Graft. Cellular Proliferation and Differentiation in the Xerogeneic Periosteal"Annals of Plastic Surgery. 48(5). 539-545 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Sawaki, N.Mizukawak, T.Yamaai, T.Yoshimoto, M.Nakano, T.Sugahara: "High Concentration of beta-Defensin-2 in Oral Squamous Cell Carcinoma."Anticancer Research. 22. 2103-2108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizukawa N., Sawaki K., Nagatsuka H., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T.: "Presence of human beta-defensin-2 in oral lichen planus and its histamine releasing effect."Asian J Oral Maxillofac Surg. 15(2). 112-117 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshimoto T, Yamaai T, Mizukawa N, Sawaki K, Nakano M, Yamachika E, Sugahara T.: "Different Expression Patterns of beta-Defensins in Human Squamous Cell Carcinomas."Anticancer Research. 23. 4629-4634 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Sawaki, T.Yamaai, N.Mizukawa, T.Yoshimoto, T.Ueno, M.Nakano, T.Sugahara: "Mortality of human epidermal keratinocytes in co-culture with oral squamous cll carcinoma cells."Anticancer Res.. 23. 79-84 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Kagawa T, Kanou M, Fukunaga J, Fujii T, Mizukawa N, Sugahara T, Yamamoto T.: "Vascular Endotherial Growth Factor (VEGF) and Bone Morphogenetic Protein-4 (BMP-4) in Endochondral Ossification from Grafted Periosteum."Acta Histochem.Cytochem.. 36(1). 61-66 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] FUKUNAGA J., YAMAAI T., YAMACHIKA E., ISHIWARI Y., TSUJIGIWA H., SAWAKI K., Y.J.Lee, UENO T., KIRINO S., MIZUKAWA N., TAKAGI S., NAGAI N., SUGAHARA T.: "Expression of Osteoclast Differentiation Factor (ODF) and Osteoclastogenesis Inhibitory Factor (OCIF) in Rat Osteoporosis Induced by Immunosuppressant FK506"BONE. 34. 425-431 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Kagawa T, Kanou M, Fukunaga J, Fujii T, Mizukawa N, Sugahara T, Yamamoto T.: "Immunohitstochemical observation of cellular origin and proliferation in endochondral bone formation from rafted periosteum."Journal of CranioMaxillofacial Surgery. 31. 356-361 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Kagawa T, Kanou M, Fukunaga J, Fujii T, Mizukawa N, Sugahara T, Yamamoto T.: "Pathology of the temporomandibular joint of patients with rheumatoid arthritis. -Case reports of secondary amyloidosis and macrophage populations."Journal of CranioMaxillofacial Surgery. 31. 252-256 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueno T, Kagawa T, Kanou M, Fukunaga J, Fujii T, Mizukawa N, Sugahara T, Yamamoto T: "Pathological change of articular cartilage in the mandibular head treated with immunosuppresant FK506."Histology and Histopathology. 19. 15-21 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizukaw N., Sugahara T.: "Presence of human β defensin-2 in oral lichen planus and its histamine releasing effect"Asian J Oral Maxillofac Surg. 15. 112-117 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshimoto T, Yamaai T, Mizukawa N, Sugahara T: "Different Expression Patterns of beta-Defensins in Human Squamous Cell Carcinom as"Anticancer Research. 23. 4629-4634 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawaki K., Yamaai T., Mizukawa N., Sugahara T.: "Mortality of human epidermal keratinocytes in co-culture with oral squamous cell car cinoma cells"Anticancer Research. 23. 79-84 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawaki K., Mizukawa N., Yamaai T., Sugahara T.: "High Concentration of β-Defensin-2 in Oral Squamous Cell Carcinoma"Anticancer Research. 22. 2103-2108 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizukawa N, Sugahara T: "Histamine Release from Rat Mast Cells Induced by Human α-Defensin-1 present in Jaw Cyst Fluid"Asian J Oral Maxillofac Surg. 14. 35-39 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T, Mizukawa N, Sugahara T: "Evaluation of Osteogenic/Chondrogenic cellular Proliferation in the Xenogeneic Periosteal graft.."Ann Plast Surg. 48. 539-545 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koichi Sawaki, Tomoichiro Yamaai, Nobuyoshi Mizukawa et al.: "Mortality of human epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells"Anticancer Res. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nobuyoshi Mizukawa et al.: "Presence of human beta-defensin-2 in oral lichen planus and its histamine releasing effect on isolated mast cells"Asian J Oral Maxillofac Surg. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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