| Project/Area Number |
14571894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MIYAMOTO Youji The University of Tokushima, University Hospital, Assistant Professor, 医学部・歯学部附属病院, 講師 (20200214)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Masaaki The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (00304535)
SATOMURA Kazuhito The University of Tokushima, University Hospital, Assistant Professor, 医学部・歯学部附属病院, 講師 (80243715)
ISHIKAWA Kunio Kyushu University, Faculty of Dental Science, Professor, 大学院・歯学研究科, 教授 (90202952)
FUJISAWA Kenji The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (40228979)
KON Masayuki The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (80116813)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | calcium phpsphate / porous ceramics / hydroxypatite / bone marrow cell / carbonate apatite / biometerials / リン酸カルシウムセラミックス / 連続気孔 / 培養骨髄細胞 |
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| Research Abstract |
The purposes of this study are (1) to fabricate the porous ceramics for bone regeneration using biodegradable calcium phosphate and (2) to evaluate bone regeneration by the ceramics and cultured bone marrow cells in vivo and in vitro.
Porous biodegradable calcium phosphate ceramics were fabricated by following methods : the polyurethane foams with different pore size were impregnated with hydroxyapatite or carbonate apatite solution. By the centrifugal operation, the specimens dried, after the surplus slurry was removed, and they were fired. On a part of the specimens, the impregnation, drying, and firing process was repeated. The specimens were observed by scanning electron microscopy. As the result, the trabecula of the ceramics thickened, and the pore size decreased with the increase of the cycles. This result indicates that average diameter of the ceramic trabecula, diameter of the pore and porosity of the ceramics could be adjusted by the number of the cycles.
Rat marrow cells were cultured for 2 weeks with in medium supplemented with ascorbic acid, beta-glycerophosphate, and dexamethasone. Cell proliferation, alkaline phosphatase activity, type I collagen synthesis, and osteocalcin production were measured, and the osteogenesis in vitro was evaluated. As the result, the osteogenesis in vitro was promoted with the increase in the pore size under 100 _m, however, the pore size over 100_m, did not affect the osteogenesis.
The ceramics/marrow cell complex cultured for 2 weeks were implanted into 7-week-old syngeneic rats. The osteogenesis in vivo was histologically evaluated at 8 weeks after the implantation. As the result, pore sizes between 100 to 200 U m were optimum. However, the absorption of ceramics in this period was slight. Further studies of the long-term resorption of the ceramics and stability of the bone will be needed.
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