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Establishment of isolation / preservation / proliferation method of osteoblast from a human bone marrow mesenchymal system cell, and potency of clinic development

Research Project

Project/Area Number 14571910
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

YAMAZAKI Yauharu  Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 講師 (00210401)

Co-Investigator(Kenkyū-buntansha) SHIMAKURA Yasuhito  Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 助手 (80255322)
OIDA Shinichiro  Turumi Univ., School of Dentistry, Associate Professor, 歯学部, 助教授 (10114745)
SEZAKI Kouichirou  Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 講師 (20216542)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordstissue engineering / human bone marrow cell / rhBMP-2 / hydroxyapatite / autologous serum culture
Research Abstract

1. Examination of utility as a transplantation bone by human bone marrow mesenchymal system cell : Human iliac cancellous bone was harvested for donor, and an obtained bone marrow was cultivated primarily, and this bone marrow mesenchymal system cell was proleferated on porous hydroxyapatite, and a substitute transplantation bone was made. We transplanted this substitute bone in an animal (nude mouse) and searched viability of transplanted bone tissue. 2. The methods for isolatation of osteoblast from a human bone marrow mesenchymal system cell preservation of it and, proliferation : Once Osteoblast from human iliac cancellous bone was isolateed after primary culture and it was cryopreserved, then the osteoblast proliferated after cryopreservation again for several years, and the viability of osteoblast was observed by animal experiment. 3. Primary culture with human bone marrow mesenchymal system cell and human osteoblast by self-serum, and the proliferation method : Bone marrow cell … More obtained from human ileum was cultivated in autologous serum component culture media or fetal bovine serum (FBS) component culture media, and cellular proliferation potency of each and osteogenic cell activity were compared.
Results and consideration : A cryopreserved and re-cultivated human bonemarrow mesenchymal system cell was seeded in porous hydroxyapatite, and mature bone formation was confirmed in vivo by adding BMP in it. Therefore, the method to make the substitute transplantation bone which an autogenous bone mallow mesenchymal system cell is defrosted and seeded on porous hydroxyapatite(+BMP) after re-culture, it was thought that we could clinically use the substitute transplantation bone by dosage of BMP and adjustment with porous hydroxyapatite in future. In addition, the efficiency was good, and the autologous serum component culture media was able to cultivate the cell which formed a bone than FBS component culture media. It seemed that this cultural system were suitable to form a bone. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Sakai N, Nakakita N, Yamazaki Y, et al.: "Oral-Facial-Digital Syndrome Type II(Mohr Syndrome) : Clinical and Genetic Manifestations"Journal of Craniofacial Surgery.. 13(2). 321-326 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 田中英子, 府川俊彦, 小林真司, 山崎安晴: "Le Fort III型骨延長後に上下顎移動術を施行したCrouzon病の1例"日本顎変形症学会雑誌. 12(3). 103-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimakura Y, Yamzaki Y et al.: "Experimental Study on Bone Formation Potential of Cryopreserved Human Bone Marrow Mesenchymal Cell/Hydroxyapatite Complex in the Presence of rhBMP2"Journal of Craniofacial Surgery.. 14(1). 108-116 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Stenport.V.F., Yamasaki Y., et al.: "FGF-4 and titanium implants : a pilot study in rabbit"Clinical Oral Implants Research. 14(3). 363-368 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai N., Nakakita N., Yamazaki Y., Ui K., Uchinuma E: "Oral-Facial-Digital Syndrome Type II (Mohr Syndrome) : Clinical and Genetic Manifestations."J Craniofac Surg.. 13(2). 321-326 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka A., Fukawa T., Kobayasi S., Yamazaki Y., Torikai K.: "A case report of Crouzon disease treated by two-jaw Surgery after Le Fort III osteotomy and bone distractions."Jpn.J.Jaw Deform.. 12(3). 103-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimakura Y., Yamzaki Y., Uchinuma E.: "Experimental Study on Bone Formation potential of Cryopreserved Human Bone marrow mesenchymal Cell/Hydroxyapatite complex in the Presence of recombinant human bone morphogenetic protein-2."J Craniofac Surg.. 14(1). 108-116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Stenport V.F., Johansson C.B., Sawase T., Yamasaki Y., Oida S.: "FGF-4 and titanium implants : a pilot study in rabbit bone."Clin.Oral Impl.Res.. 14(3). 363-368 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai N, Nakakita N, Yamazaki Y, et al.: "Oral-Facial-Digital Syndrome Type II (Mohr Syndrome) : Clinical and Genetic Manifestations."Journal of Craniofacial Surgery.. 13(2). 321-326 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田中英子, 府川俊彦, 小林真司, 山崎安晴: "Le Fort III型骨延長後に上下顎移動術を施行したCrouzon病の1例"日本顎変形症学会雑誌. 12(3). 103-112 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimakura Y, Yamzaki Y et al.: "Experimental Study on Bone Formation Potential of Cryopreserved Human Bone Marrow Mesenchymal Cell/Hydroxyapatite Complex in the Presence of rhBMP2"Journal of Craniofacial Surgery.. 14(1). 108-116 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Stenport.V.F., Yamasaki Y., et al.: "FGF-4 and titanium implants : a pilot study in rabbit"Clinical Oral Implants Research. 14(3). 363-368 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakai N, Hasegawa H, Yamazaki Y et al.: "A case of a Japanese patient with cleidocranial dysplasia possessing a mutation of CBFA1 gene"Journal of Craniofacial Surgery. 13(1). 31-34 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai N, Nakakita N, Yamazaki Y, et al.: "Oral-Facial-Digital Syndrome Type II (Mohr Syndrome) : Clinical and Genetic Manifestations"Journal of Craniofacial Surgery. 13(2). 312-326 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 田中英子, 府川俊彦, 小林真司, 山崎安晴: "Le Fort III型骨延長後に上下顎移動術を施行したCrouzon病の1例"日本顎変形症学会雑誌. 12(3). 103-112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 池本繁弘, 山崎安晴: "下顎窩骨折の3例"日本頭蓋顎顔面外科学会雑誌. 18(3). 272-278 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimakura Y, Yamzaki Y et al.: "Experimental Study on Bone Formation Potential of Cryopreserved Human Bone Marrow Mesenchymal Cell/Hydroxyapatite Complex in the Presence of rhBMP2"Journal of Craniofacial Surgery. 14(1). 108-116 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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