| Project/Area Number |
14571910
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
YAMAZAKI Yauharu Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 講師 (00210401)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAKURA Yasuhito Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 助手 (80255322)
OIDA Shinichiro Turumi Univ., School of Dentistry, Associate Professor, 歯学部, 助教授 (10114745)
SEZAKI Kouichirou Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 講師 (20216542)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | tissue engineering / human bone marrow cell / rhBMP-2 / hydroxyapatite / autologous serum culture |
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| Research Abstract |
1. Examination of utility as a transplantation bone by human bone marrow mesenchymal system cell : Human iliac cancellous bone was harvested for donor, and an obtained bone marrow was cultivated primarily, and this bone marrow mesenchymal system cell was proleferated on porous hydroxyapatite, and a substitute transplantation bone was made. We transplanted this substitute bone in an animal (nude mouse) and searched viability of transplanted bone tissue. 2. The methods for isolatation of osteoblast from a human bone marrow mesenchymal system cell preservation of it and, proliferation : Once Osteoblast from human iliac cancellous bone was isolateed after primary culture and it was cryopreserved, then the osteoblast proliferated after cryopreservation again for several years, and the viability of osteoblast was observed by animal experiment. 3. Primary culture with human bone marrow mesenchymal system cell and human osteoblast by self-serum, and the proliferation method : Bone marrow cell
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obtained from human ileum was cultivated in autologous serum component culture media or fetal bovine serum (FBS) component culture media, and cellular proliferation potency of each and osteogenic cell activity were compared.
Results and consideration : A cryopreserved and re-cultivated human bonemarrow mesenchymal system cell was seeded in porous hydroxyapatite, and mature bone formation was confirmed in vivo by adding BMP in it. Therefore, the method to make the substitute transplantation bone which an autogenous bone mallow mesenchymal system cell is defrosted and seeded on porous hydroxyapatite(+BMP) after re-culture, it was thought that we could clinically use the substitute transplantation bone by dosage of BMP and adjustment with porous hydroxyapatite in future. In addition, the efficiency was good, and the autologous serum component culture media was able to cultivate the cell which formed a bone than FBS component culture media. It seemed that this cultural system were suitable to form a bone. Less
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