| Project/Area Number |
14571914
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
YAMAUCHI Tomohoro TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSISTANT PROFFESSOR, 歯学部, 助手 (60328276)
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| Co-Investigator(Kenkyū-buntansha) |
KATAKURA Akira TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, LECTURER, 歯学部, 講師 (10233743)
SHIBAHARA Takahiko TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFFESSOR, 歯学部, 教授 (50178919)
MURAMATSU Takashi TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, LECTURER, 歯学部, 講師 (00276982)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Oral Cancer / Precancerous resion / Leukoplakia / Doc-1 |
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| Research Abstract |
I.Expression of Doc-1 protein
Expression of Doc-1 protein was investigated in 40 patients with leukoplakia of the oral cavity, primarily using tissue samples of atypical epithelia.
1.Methods
1)Western blotting
Collected tissue samples were crushed in RIP buffer and centrifuged. Supernatant was then subjected to SDS-PAGE using polyamide gel. Protein in the gel was transferred to a PVDF membrane and the presence and changes in Doc-1 protein were ascertained using the alkaline phosphatase method. Anti-actin antibody was used as a control sample.
2)Immunohistochemical staining
Collected tissue samples were frozen and sliced, and according to the indirect fluorescent antibody method, anti-mouse Doc-1 polyclonal antibody was used as the primary antibody. FITC-labeled antibody was then used as the secondary antibody to detect primary antibody.
2.Results and Discussion
-In patients in whom leukoplakia became cancerous during the study, Doc-1 protein was detected in both Experiments 1 and 2.
-In patients in whom leukoplakia did not change during the study, Doc-1 protein was detected only in Experiment 2.
-In patients in whom dysplasia advanced during the study, no defined tendencies were seen in Experiments 1 and 2.
The results of this study suggest that if Doc-1 protein is seen by Western blotting or immunohistochemical staining on initial presentation or during follow-up in patients with leukoplakia of the oral cavity, dysplasia is likely to advance and become cancerous.
II.Changes in salivary cytokines in patients with leukoplakia of the oral cavity
Levels of salivary IL-6 and -8 were measured in 32 patients with leukoplakia of the oral cavity, and results were compared to levels in healthy individuals.
1.Methods
ELISA was performed.
2.Results and Discussion
Levels of IL-8 were significantly higher for patients with leukoplakia of the oral cavity than for healthy individuals.
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