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Gene expressions of BMP signaling in osteoclasts by means of electron microscopy^*

Research Project

Project/Area Number 14571916
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNihon University

Principal Investigator

TERAKADO Masaaki  Nihon University, School of Dentistry, Department of Oral and Maxillofacial Surgery, Associate Professor, 歯学部, 助教授 (60059904)

Co-Investigator(Kenkyū-buntansha) OIDA Shinichiro  Tsurumi University, School of Dental Medicine, Department of Biochemistry, Associate Professor, 歯学部, 助教授 (10114745)
INAGE Toshihiko  Nihon University, School of Dentistry, Department of Anatomy, Associate Professor, 歯学部, 助教授 (90096769)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsosteoclast / osteoblast / BMP / TGF-β / Smad / recepto / receptor / 骨形成 / 線維芽細胞
Research Abstract

BMP plays an important role during the healing process of extraction tooth sockets, acting as a paracrine or autocrine. BMP receptor type-lB is closely related to bone formation. In order to demonstrate the function of BMP during bone regeneration, the expression of BMP receptor mRNA was revealed by means of in situ hybridization (ISH) using a light and electron microscope during. bone healing process.
By light microscopic ISH, one day after tooth extraction the signals of BMPR-IB mRNA were localized in the osteoclasts and fibroblast-like cells. Two days after tooth extraction, the signals were found in the osteoclasts and fibroblasts, with the most intense expression found in the osteoclasts. Signals were detected in the fibroblasts across a wider area of the extraction sockets at 3 days. Gene expression was not detected 4 days after extraction.
By electron microscopic ISH, the signals of BMPR-IB mRNA were localized on the attached ribosomes of the osteoclasts and fibroblasts. Two days after extraction the signals of the cellular element were stronger than those at 1 days. From 3 to 4 days after tooth extraction, the osteoblasts showed their most intense signals. Weak signals were still detected in the osteoblasts 14 days after tooth extraction.
There were no signals in the cellular element of 1511 using the sense RNA strand.
BMP may stimulate bone resorption by osteoclasts before fibrogenesis and osteogenesis. The detection of signals with electron microscopic ISH was more effective than light microscopic ISH.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 稲毛稔彦, 寺門正昭他8名: "電子顕微鏡を用いたエナメル芽細胞におけるamelogenin mRNAの発現"日大歯学. 76. 491-499 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 稲毛稔彦, 寺門正昭他15名: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. 77. 141-149 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 稲毛稔彦 他: "歯周組織とエナメルタンパク"永末書店. 61-69 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] "Expression of amelogenin mRNAin ameloblasts revealed by electron microscopy"Nihon Univ Dent J. vol.74. 491-499 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] "Distributions of CBFA-1 and BMP-4 during development of oral maxillofacial region in mice"Nihon Univ Dent J. vol.77. 141-149 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 桑田文幸, 堀稔, 関和忠信, 寺門正昭, 稲毛稔彦, 佐藤慶伴, 小方頼昌, 福島一雅, 田中博, 大井田新一郎: "組織工学による骨誘導に関する研究"日本大学歯学部研究紀要(一般教育). 30号. 37-48 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 稲毛稔彦, 大井田新一郎, 寺門正昭他: "抜歯窩の治癒過程におけるBMPの遺伝子発現"日本大学歯学部総合歯学研究所研究報告書. 03号. 101-111 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲毛稔彦, 他: "歯胚の成長に伴う硬組織形成の微細構造"日本大学歯学部研究紀要(一般教育). 29号. 45-53 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲毛稔彦: "歯科におけるBMPと骨誘導の発展性"日本歯科医師会雑誌. 53・11. 1029-1036 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲毛稔彦, 寺門正昭, 他: "電子顕微鏡を用いたエナメル芽細胞におけるamelogeninmRNAの遺伝子発現"日大歯学. 76・5. 491-499 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲毛稔彦, 寺門正昭, 大井田新一郎他: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. (発表予定).

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲毛稔彦他: "歯周組織とエナメルタンパク"永末書店. 61-69 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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