| Project/Area Number |
14571916
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
TERAKADO Masaaki Nihon University, School of Dentistry, Department of Oral and Maxillofacial Surgery, Associate Professor, 歯学部, 助教授 (60059904)
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| Co-Investigator(Kenkyū-buntansha) |
OIDA Shinichiro Tsurumi University, School of Dental Medicine, Department of Biochemistry, Associate Professor, 歯学部, 助教授 (10114745)
INAGE Toshihiko Nihon University, School of Dentistry, Department of Anatomy, Associate Professor, 歯学部, 助教授 (90096769)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | osteoclast / osteoblast / BMP / TGF-β / Smad / recepto / receptor / 骨形成 / 線維芽細胞 |
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| Research Abstract |
BMP plays an important role during the healing process of extraction tooth sockets, acting as a paracrine or autocrine. BMP receptor type-lB is closely related to bone formation. In order to demonstrate the function of BMP during bone regeneration, the expression of BMP receptor mRNA was revealed by means of in situ hybridization (ISH) using a light and electron microscope during. bone healing process.
By light microscopic ISH, one day after tooth extraction the signals of BMPR-IB mRNA were localized in the osteoclasts and fibroblast-like cells. Two days after tooth extraction, the signals were found in the osteoclasts and fibroblasts, with the most intense expression found in the osteoclasts. Signals were detected in the fibroblasts across a wider area of the extraction sockets at 3 days. Gene expression was not detected 4 days after extraction.
By electron microscopic ISH, the signals of BMPR-IB mRNA were localized on the attached ribosomes of the osteoclasts and fibroblasts. Two days after extraction the signals of the cellular element were stronger than those at 1 days. From 3 to 4 days after tooth extraction, the osteoblasts showed their most intense signals. Weak signals were still detected in the osteoblasts 14 days after tooth extraction.
There were no signals in the cellular element of 1511 using the sense RNA strand.
BMP may stimulate bone resorption by osteoclasts before fibrogenesis and osteogenesis. The detection of signals with electron microscopic ISH was more effective than light microscopic ISH.
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