| Project/Area Number |
14571917
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
SEKIWA Tadanobu Nihon University, Department of Oral and Maxillofacial Surgery, assistant, 歯学部, 助手 (00154659)
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| Co-Investigator(Kenkyū-buntansha) |
OIDA Shinichiro Tsurumi University, School of Dental Medicine, Department of Biochemistry, Associate Professor, 歯学部, 助教授 (10114745)
INAGE Toshihiko Nihon University, School of Dentistry, Department of Anatomy, Associate Professor, 歯学部, 助教授 (90096769)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Tissue engineering / BMP / Growth factor / Bone induction / mRNA / TGF-β / RANKL / OPG / CBFA-1 / Smad |
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| Research Abstract |
Recently, therapeutics using growth factors and extracellular matrix such as EMDOGAIN or BMP has been introduced dental practice : The growth factors therapeutics need a large amount of proteins and the mechanisms of healing are still obscure. In the present study, bone marrow. stroma cells and HAP were used for bone formation of tissue engineering.
Materials and Methods
Wister rats weighting 100g were used for this study. The stroma cells of bone marrow were isolated from bone marrow by aspiration of syringe. Stroma cells were cultured in alpha-MEM medium with HAP or CPC (calcium phosphate cement) by adding BMP. Stroma cells were cultured for 1 week. Then, cultured cells-HAP complex were transplanted in the artificial hole of calvaria.
Results
The cell-CPC complex showed stronger bone formation than that of cell-HAP complex. After 2 week of implantation of cell-CPC, thick layer of connective tissue was covered on implants. After 4 week the layer was calcified. Hence, in the implantation of the connective tissue layer was formed at 4 weeks after the implantation. TGF-bet a superfamily was expressed in the preosteoblasts, fibroblasts and osteoclasts around implants. These data suggests that TGF-beta superfamily plays central role in ectopic bone formation.
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