| Project/Area Number |
14571947
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
矯正・小児・社会系歯学
|
|---|
| Research Institution | OKAYAMA UNIVERSITY |
|---|
Principal Investigator |
MIYAMOTO Manabu Okayama Univ., Graduate Sch. Medicine and Dentistry, Instructor, 大学院・医歯学総合研究科, 助手 (40252978)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Teruko (TAKANO Teruko) Okayama Univ., Graduate Sch. Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (00127250)
TUBOI Yoshiko Okayama Univ., Medical and Dental Hosp., Instructor, 医学部・歯学部附属病院, 助手 (50325122)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | periodontal ligament / cell culture / FGF / 歯根膜 / 遺伝子発現 |
|---|
| Research Abstract |
In order to determine specific gene expression on periodontal ligament cells, we analyzed the change of gene expression after stimulation against the cloned cells that were derived from single-cell. We only prepared limited amount of human periodontal ligament cells which were derived from single-cell by using popular media. Then we had to add several growth factors,into media to maintain long term-culture. We analyzed the effects of fibroblast growth factor-2 (FGF-2) on proliferation and replicative capacity in human periodontal cells. Addition of FGF-2 on logarithmically growing cell enhanced proliferation of cell number by 10 to 15 %. FGF-2 also enhanced colony forming capacities on cultured cells in the limited dilution methods. These effects did not change between young and old cells, and suggested same throughout the life-span of cultured cells. We analyzed the effect on FGF-2 to replicative capacity in continuous long-term cell culture. Addition of FGF-2 enhanced proliferation in early stage of life-span, however, it caused inhibition of proliferation at late stage of life span. Totally, addition of FGF-2 caused decreased replicative capacity. We also determined gene expression levels of p53 and p16 into the cultured cells at different stage of life-span by semi-quantified RT-PCR method, and identified no significant differences into the cells. These results suggest that FGF-2, which was known a potent mitogen for several kind of cells, has reverse effect during long-term culture.
|
