| Project/Area Number |
14571961
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
矯正・小児・社会系歯学
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
MAKI Kenshi Kyushu Dental College, Pediatric Dentistry, Associate Professor, 歯学部, 助教授 (60209400)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Mitsutaka Kyushu Dental College, Pediatric Dentistry, Professor, 歯学部, 教授 (70047801)
NOGUCHI Jiroo Kyushu Dental College, Biochemistry, Professor, 歯学部, 教授 (30073688)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | Odontollast / Matrix Vesicle / Protease / Lactate Dehydrogenate / Matrix Vesicle / 歯根膜 / protease / Metalloprotease / Alkaline phosphataes |
|---|
| Research Abstract |
Matrix vesicles are extracellular organelles that initiate mineralization in the extracellular that initiate mineralization in the extracellular matrix of carious calcifying tissues. The matrix vesicles fractions from the dentin of fetal bovine, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation.
In this report, we investigated the enzymes alkaline phosphates activity( ALP), protease activity lactate dehydrogenase(LDH) and protease to evaluate the matrix vesicles. We found not only matrix vesicles but also matrix vesicle like vesicles without protease in the dentin of fetal bovine. Protease activity after inhibition with o-phenanthroline was restored by the addition of Co<2+>, A1<2+>, Zn<2+>, Mg<2+>,. These results show that the protease in the matrix vesicles in the dentin is a metalloprotease.
|
