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Interleukin-6 stimulates cathepsin B and L activities of human periodontal ligament cells through the signaling pathways

Research Project

Project/Area Number 14571969
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionNihon University

Principal Investigator

YAMAGUCHI Masaru  Nihon University, School of Dentistry at Matsudo, Lecturer(Full-Time), 松戸歯学部, 講師 (60333100)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsHuman periodontal ligament cells / Tension force / Compression force / Gingival crevicular fluid / Cathepsin B, L / Experimental tooth movement / Interleukin-6 / 培養ヒト歯根膜線維芽細胞 / カテプシンB、L / 矯正学的歯の移動 / カテプンB, L
Research Abstract

Our in vitro study examined the secretion of cathepsin B(CAB) and cathepsin L(CAL) in PDL cells following mechanical stress. PDL cells were subjected to 0.5, 1.0, 2.0, or 3.0 g/cm^2 of compression force or an increase in surface area by tension force of 0.28%, 0.95%, 1.72%, or 2.50% for 24 hr. We found compression and tension significantly increased the secretions of both CAB and CAL in PDL cells, which were exhibited in a time- and force magnitude-dependent manner. The compression-stimulated secretion of CAB was increased approximately 3-fold and that of CAL 4-fold, as compared with the control. Further, tension-stimulated secretion of CAB was increased by 1.5-fold and that of CAL 2-fold compared with the control. When analyzed using a semiquantitative polymerase chain reaction(RT-PCR) assay, CAB and CAL mRNA were increased in response to both compression and tension forces. These findings demonstrated that mechanical stress (compression and tension forces) causes an increase in secretion of CAB and CAL in PDL cells in vitro.
Furthermore, we examined that the mechanisms controlling CAB and CAL activities and intracellular signaling by IL-6 in orthodontic tooth movement. Cultured PDL cells were treated with different concentrations (0.01-10 ng/ml) of IL-6 in the absence or presence of the p38 MAP kinase inhibitor SB-208350 or the p42/44 inhibitor PD-98059. Protein and gene expression of CAB and CAL were determined by ELISA and PCR, respectively. SB-208350 and PD-98059 inhibited the increase in CAB and CAL production induced by IL-6. These results suggest the IL-6 regulates muscle CAB and CAL production at least in part by activating the MAP kinase pathway and NF-kappaB.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (13 results)

All 2005 2004 2003 Other

All Journal Article (8 results) Publications (5 results)

  • [Journal Article] The evaluation of OGES in mandibular incisor with mandibular prognathism following orthognathic surgery2005

    • Author(s)
      Masaru Yamaguchi
    • Journal Title

      Dentistry in Japan (印刷中)

    • NAID

      80015924964

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Levels of t-PA and PAI-2 in gingival crevicular fluid during orthodontic tooth movement in adults2005

    • Author(s)
      Junko Hoshino-Itoh
    • Journal Title

      Australian Orthodontic Journal (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prediction and causes of open gingival embrasure space in anterior teeth of the mandibule following orthodontic treatment2004

    • Author(s)
      Tadataka Ikeda
    • Journal Title

      Australian Orthodontic Journal 21(1)

      Pages: 87-92

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cathepsins B and L Are Increased in the Response of Periodontal Ligament Cells to Mechanical Stress in Vitro2004

    • Author(s)
      Masaru Yamaguchi
    • Journal Title

      Connective Tissue Research 45

      Pages: 181-189

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neuropeptides stimulate production of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in human dental pulp cells2004

    • Author(s)
      Masaru Yamaguchi
    • Journal Title

      Inflammation Research 53(5)

      Pages: 199-204

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The level of cathepsin B in gingival cervicular fluid during human orthodontic tooth movemen2003

    • Author(s)
      Yuriko Sugiyama
    • Journal Title

      European journal of Orthodontics 25

      Pages: 71-76

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inflammation in periodontal tissues in response to mechanical forces. (Review)

    • Author(s)
      Masaru Yamaguchi, Kazutaka Kasai
    • Journal Title

      Archivum Immunologiae et Therapiae Experimentalis (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inflammation in periodontal tissues in response to mechanical forces. (Review)

    • Author(s)
      Masaru Yamaguchi, Kazutaka Kasai
    • Journal Title

      Archivum Immunologiae et Therapiae Experimentalis (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Satoh Hidemasa: "Relationships between masticatory function and internal structure of mandible based on CT finding"Am J Orthod Dentof Orthop. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Sugiyama Yuriko: "The level of cathepsin B in gingival crevicular fluid during human orthodontic tooth Movement"Eur J Orthod. 25(1). 71-76 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nozoe Tomomi: "The level of cathepsin L in gingival crevicular fluid during orthodontic tooth movement and effect of tension-force on cathepsin L activity from periodontal ligament cells"Orthod Waves. 61(6). 447-453 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山口 大: "矯正治療による重度歯根吸収者由来歯髄細胞におけるneuropeptideによる炎症性サイトカイン産生能"第61回日本矯正歯科学会大会・抄録集. 174 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ko-Kimura Natsue: "Some factors associated with to the occurrence of open gingival embrasures following orthodontic treatment"Aust Orthod J. (In press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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