| Project/Area Number |
14571990
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
NUMABE Yukihiro The Nippon Dental University, School of Dentistry at Tokyo, Associate Professor, 歯学部, 助教授 (90198557)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Tomohisa The Nippon Dental University, School of Dentistry at Tokyo, Assistant Professor, 歯学部, 講師 (20307961)
SUZUKI Asami The Nippon Dental University, School of Dentistry at Tokyo, Assistant, 歯学部, 助手 (60318540)
HISANO Akiko The Nippon Dental University, School of Dentistry at Tokyo, Assistant, 歯学部, 助手 (20267305)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Neutrophils / Saliva / Periodontal tissue / Periodontal disease / Flow Cytometry / Cytometry |
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| Research Abstract |
PMNs are associated with host defense mechanism and tissue destruction in periodontal tissue. Alterations of PMN functions such as phagocytosis, chemotaxis, oxidative burst, enzyme release and F-actin formation play a pivotal role in periodontal pathogenesis. Furthermore, one of the major end-products of PMN activity is the extracellular release of active proteases. PMNs contain large amounts of neutral serine protease such as elastase, cathepsin and collagenase, these enzymes can degrade many important proteins in the extracellular matrix of periodontal tissue. In recent years, it has been suggested that PMN apoptosis plays a role in the regulation of inflammation in periodontal tissue and control the host immune response. Our study focused on the following themes to investigate the possibility of Salivary PMNs (SPMNs) as a marker for periodontal disease. Alteration of SPMN phagocytic functions and enzyme activities in periodontal disease patients and Smokers. Status of PMN apoptosis expression in saliva and the relationship between effects of various factors around these cells (e.g. fluid, cytokine) and apoptosis induction. We employ a flowcytometry, cytoenzymology and laser scanning microscopy technique to investigate these phenomena. In summary, these results indicate that alteration of environment around PMNs in saliva is significantly related to the change of PMN functions and progression of periodontitis. Examination of PMNs in Saliva may be important factor for detecting the periodontal disease progression.
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