| Project/Area Number |
14572017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Nihon University |
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Principal Investigator |
KITANAKA Susumu Nihon University, College of Pharmacy, Department of Pharmacognosy, Professor, 薬学部, 教授 (40102553)
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| Co-Investigator(Kenkyū-buntansha) |
DAIKONYA Akiliiro Nihon University, College of Pharmacy, Department of Pharmacognosy, assistant, 薬学部, 助手 (60328763)
MIYATA Syohei Nihon University, Department of Chemistry, College of Humanities and Sciences, assistant professor, 文理学部, 助教授 (30090038)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Euphorbia kansui / Euphorbiaceae / ingenane / jatrophane / euphane / tirucallane / animal cap assay / anti-cancer / apoptosis / Euphorbia Kansui / iugenane / jatrophane / animal cap assay / cell cleavage arrest |
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| Research Abstract |
The result in this research is as follows.
1. Compound separated from kansui :
On the basis of a bioassay-guided fractination, 21 sorts of ingenol type diterpenes which containing ten new compounds, five jatrophane type diterpenes which containing three new compounds, seven euphane/tirucallane type triterpenes which containing five new compounds, five steroid, one cumarin, one fatty acid, a total of 38 sorts of compounds were isolated and identified.
2. Ingenol type diterpenes showed the strongest cell division inhibitory activity in the above-mentioned compounds. Moreover, these compounds controlled cell division and the cells survived.
3. As a result of the selective inhibition of the growth of cancer cells (MMT, LC540, and 3T3) by ingenane-type diterpenes. 20-O-(2¢ ; E, 4¢ ; Z-decadienoyl) ingenol. (2) did not show a inhibition control to 3T3 cell which is a normal cell, but it showed the alternative inhibitory control action to the breast cancer cell MMT.
4. Ingenol type diterpene 3-O-(2, 3-dimethylbutanoyl)-13-O-dodecanoylingenol (20) showed a cell growth inhibitory -activity remarkably to Jurkat and the HSC-2 cell. And the findings suggest 20 mainly induced early apoptosis in HSC-2 cells
5. In the above results, ingenol type diterpene in kansui induced the early apotosis and inhibited cell division of some cancer-cells. It is thought that these bioactive components have a highly possibility to be an anti-cancer drug with a new action mechanism.
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