EFFECTS OF GLYCOSIDASE INHIBITORY SUGAR-MIMIC ALKALOIDS AGAINST LYSOSOME DISEASE.
| Project/Area Number |
14572053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
ADACHI Isao TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, UNIVERSITY HOSPITAL, PROFESSOR, 附属病院, 教授 (30151070)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Atsushi TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, UNIVERSITY HOSPITAL, INSTRUCTOR, 附属病院, 助手 (60303236)
ASANO Naoki HOKURIKU UNIVERSITY, FACULTY OF PHARMACEUTICL SCIENCES, ASSOOATE PROFESSOR, 薬学部, 助教授 (50121265)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | LYSOSOME DISEASE / GLUCOCEREBROSIDE / SUGAR-MIMIC ALKALOIDS / 糖鎖生合成 |
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| Research Abstract |
Gaucher disease is a disorder of glycosphingolipid metabolism caused by deficiency of lysosomal β-glucocerebrosidase. Studies of residual activities of mutant enzyme in many Gaucher patients showed that some of them had kinetic properties similar to those for nomal β-glucocerebrosidase, but were significantly less stable. So, we searched the compounds, which stabilized the mutant β-glucocerebrosidase. A very thorough examination of the roots of Lycium chinense(Solanaceae) has resulted in the discovery of ten calystegines. Calystegine A_3,B_1,B_2 and C_1 is a potent competitive inhibitor of β-glucocerebrosidase, with K_i, value of 0.9,1.4,1.5,and 0.49μM, respectively. We found that those calystegines, potent competitive inhibitors of β-glucocerebrosidase, effectively enhanced β-glucocerebrosidase activity in Gaucher lymphoblast, when administrated at concentrations lower than that usually requied for intercellular inhibition of the enzyme. The enzyme activity increased two or three-fold after cultivation of Gaucher lymphoblast(L444P) with calystegine at 10μM for 3 days. Those calystegines seemed to accelerate transport and maturation of the mutant enzyme. Our results indicate that administration of calystegines may be used as a new molecular therapy for Gaucher disease.
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Report
(3 results)
Research Products
(7 results)
