| Project/Area Number |
14572060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | NAGOYA CITY UNIVERSITY |
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Principal Investigator |
MIZUTANI Takaharu NAGOYA CITY UNIVERSITY, 大学院・薬学研究科, 教授 (30080188)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kazuhiko NAGOYA CITY UNIVERSITY, GRADUATE SCHOOL OF PHARMACEUTICAL SCIENCES, ASSOCIATE PROFESSOR, 大学院・薬学研究科, 助教授 (40117833)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | SELENOCYSTEINE / Se-proteins / Se / tRNA / SLA / LP / グルタチオンペルオキシダーゼ / 原生動物 |
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| Research Abstract |
This contains three great results obtained in this research project, as follows:
The first is the evidence of the presence of G-A/A-G non Watson-Crick base-pairs in selenocysteine insertion sequence (SECIS) found in the 3'-untranslated region of Se-protein mRNA. This base-pair was confirmed by the Tm measurement and electrophoresis of those oligo-nucleotides of RNA and DNA containing G-A/A-G base-pair. This is a key result to understand molecular basis of mechanism of selenocysteine incorporation.
The second is the finding of the relevant Sec translational apparatus, such as Sec-tRNA and its synthetase, in a kind of Protozoa, Oxyrrhis marina. Thus, it was clarified that the four kingdoms among five kingdoms have Sec-system.
The third is some evidence of the presence of autoantibody against Sec synthetase in sera from autoimmune hepatitis patients. Sera clearly inhibited the synthesis of Sec-tRNA. In future, the role of autoantigen (SLA/LP) will be clarified.
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