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Transcriptional regulation of human sialyltransferases genes」 Summary of research results

Research Project

Project/Area Number 14572076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionNational Research Institute for Materials Science

Principal Investigator

TANIGUCHI Akiyoshi  National Research Institute for Materials Science, Biomaterials center, Senior Researcher, 生体材料研究センター, 主幹研究員 (70256759)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
KeywordsSialyltransferase / promoter / tissue specific expression / luciferase assay / glycosyltransferase gene / HNF-1 / HNF-1 / 組織特異的プロモーター / 転写調節 / DCoH / PCD
Research Abstract

1.Characterization of the promoter region of hST3Gal II and III
We identified the promoter region of hST3Gal II and III using 5'-RACE analysis, and characterized of the promoter activities of hST3Gal II and III using luciferase assay.
2.Transcriptional regulation of hST3Gal IV gene in testis and ovary cell lines.
Our results indicate the element that plays a critical role in germ-cell specific regulation of the pA promoter. The results of site-directed mutagenesis suggest that AP2 and c-Ets sites in this region are involved in pA promoter activity, which in turn suggests that the hST3Gal IV gene is regulated in a tissue-restricted fashion at the level of transcription.
3.Transcriptional regulation of hST6Gal I gene in colon adenocarcinoma cell line.
Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity about 80 % compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] T.Koike, N.Kimura, K.Miyazaki, T.Yabuta, K.Kumamoto, S.Takenoshita, J.Chen, M.Kobayashi, M.Hosokawa, A.Taniguchi, T.Kojima, N.Ishida, M.Kawakita, H.Yamamoto, H.Takematsu, Y.Kozutsumi, A.Suzuki, R.Kanangi: "Hypoxia induces adhesion molecules on cancer cells a missing link between Warburrg effect and induction of selectin ligand carbohydrates."Proc.Natl.Acad.Sci.. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ishibashi, F.Kobayashi, A.Idesawa, A.Taniguchi, S.Matsuzawa: "Effect of carbocisteine on altered activities of glycosidase and glycosyltransferse and expression of Muc5ac in SO2-exposed rats."Eur.J.Pharmacol.. 487. 7-15 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] L.Xu, Y.Kurusu, K.Takizawa, J.Tanaka, K.Matsumoto, A.Taniguchi: "Transcriptional Regulation of Human β-galactoside ・2,6-Sialyltransferase (hST6Gal I) Gene in Colon Adenocarcinoma Cell Line."Biochem.Biophys.Res.Commun.. 307. 1070-1074 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, M.Hioki, K.Matsumoto: "Transcriptional Regulation of Human Gal・1,3GalNAc/Gal・1, 4GIcNAc ・2,3-Sialyltransferase (hST3Gal IV) Gene in Testis and Ovary Cell Line"Biochem.Biophys.Res.Commun. 301. 764-768 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, K.Saito, T.Kubota, K.Matsumoto: "Characterization of the promoter region of the human Gal・1,3(4)GlcNAc ・2,3-sialyltransferase III (hST3Gal III) gene."Biochim.Biophys.Acta. 1626. 92-96 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, T.Morisima, Y.Tsujita, Y.Matsumoto, K.Matsumoto: "Genomic Structure, Expression and Transcriptional Regulation of Human Gal・1,3GalNAc ・2,3-Sialyltransferase Gene."Biochem.Biophys.Res.Commun. 300. 570-577 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Koike, N.Kimura, K.Miyazaki, T.Yabuta, K.Kumamoto, S.Takenoshita, J.Chen, M.Kobayashi, M.Hosokawa, A.Taniguchi, T.Kojima, N.Ishida, M.Kawakita, H.Yamamoto, H.Takematsu, Y.Kozutsumi, A.Suzuki, R.Kanangi: "Hypoxia induces adhesion molecules on cancer cells a missing link between Warburrg effect and induction of selectin ligand carbohydrates"Proc.Natl.Acad.Sci.USA. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ishibashi, F.Kobayashi, A.Idesawa, A.Taniguchi, S.Matsuzawa: "Effect of carbocisteine on altered activities of glycosidase and glycosyltransferse and expression of Muc5ac in SO2-exposed rats."Eur.J.Pharmacol.. 487. 7-15 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] L.Xu, Y.Kurusu, K.Takizawa, J.Tanaka, K.Matsumoto, A.Taniguchi: "Transcriptional Regulation of Human b-galactoside a2,6-Sialyltransferase (hST6Gal I) Gene in Colon Adenocarcinoma Cell Line."Biochem.Biophys.Res.Commun. 307. 1070-1074 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, M.Hioki, K.Matsumoto: "Transcriptional Regulation of Human Galb1,3GalNAc/Galb1,4GlcNAc a2,3-Sialyltransferase (hST3Gal IV) Gene in Testis and Ovary Cell Lines."Biochem.Biophys.Res.Commun. 301. 764-768 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, K.Saito, T.Kubota, K.Matsumoto: "Characterization of the promoter region of the human Galb1,3(4)GlcNAc a2,3-sialyltransferase III (hST3Gal III) gene."Biochim.Biophys.Acta. 1626. 92-96 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Taniguchi, T.Morisima, Y.Tsujita, Y.Matsumoto, K.Matsumoto: "Genomic Structure, Expression and Transcriptional Regulation of Human Galb1,3GalNAc a2,3-Sialyltransferase Gene."Biochem.Biophys.Res.Commun.. 300. 570-577 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] L.Xu, Y.Kurusu, K.Takizawa, J.Tanaka, K.Matsumoto, A.Taniguchi: "Transcriptional Regulation of Human beta-galactoside alpha2,6-Sialyltransferase (hST6Gal I) Gene in Colon Adenocarcinoma Cell Line."Biochem.Biophys.Res.Commun. 307. 1070-1074 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Ishibashi, F.Kobayashi, A.Idesawa, A.Taniguchi, S.Matsuzawa: "Effect of carbocisteine on altered activities of glycosidase and glycosyltransferse and expression of Muc5ac in SO2-exposed rats."Eur.J.Pharmacol. 248. 7-15 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Taniguchi, T.Morisima, Y.Tsujita, Y.Matsurnoto, K.Matsumoto: "Genomic Structure, Expression and Transcriptional Regulation of Human Gal 1,3GaINAc 2,3-Sialyltransferase (hST3Gal II) Gene"Biocheim. Biophys. Res Commun.. 300. 570-576 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Taniguchi, M.Hioki, K.Matsumoto: "Transcriptional Regulation of Human Gal 1,3GalNAc/Gal 1, 4GlcNAc 2,3-Sialyltransferase (hST3Gal IV) Gene in Germ Cell Drived Cell Lines"Biochem. Biophys. Res Commun. 301. 764-768 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Taniguchi, K.Saito, T.Kubota, K.Matsumoto: "Characterization of the promoter region of the human Gal 1,3(4)GlcNAc 2,3-sialyltransferase III (hST3Gal III) gene"Biochim. Biophys. Acta. (In press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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