| Project/Area Number |
14572117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
YOSHINO Shin Kobe Pharmaceutical University, Pharmacology, Professor, 薬学部, 教授 (00260729)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | genetically modified foods / oral tolerance / intestinal immunity / allergy / cytokine / Th1 / Th2 |
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| Research Abstract |
The present study was undertaken to investigate the effect of genetically modified soybeans on oral tolerance in mice that appeared to play an important role in the prevention of food allergy. The soybeans were daily given orally over an appropriate period before and after immunization of OVA and oral tolerance was induced by feeding ovalbumin(OVA) as an antigen once a day for five days before immunization with the antigen. Then 3 weeks later anti-OVA IgG production and proliferative responses of spleen cells to the antigen were determined by ELISA and intake of[^3H]-mymidine in the cells. Th1 responses including anti-OVA IgG2a and IFN-g production and Th2 responses including anti-OVA IgG1 and IL-10 were also measured. The results showed that administration of the genetically modified soybeans was followed by no effects on the dowregulation of proliferation of lymphoid cells caused by oral OVA but was effective in facilitating the suppression of anti-OVA IgG,IgG2a,IgG1 antibody production as well as IFN-g and IL-10 secretion from splenic cells. These results suggest that genetically modified soybeans used in our studies, at least, does not appear to prevent food allergy.
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