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Establishment of tumor maker test using tumor-derived nucleic acids in plasma

Research Project

Project/Area Number 14572177
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKANAZAWA UNIVERSITY

Principal Investigator

OHTAKE Shigeki  KANAZAWA UNIVERSITY, Faculty of Medicine, Professor, 医学部, 教授 (00160523)

Co-Investigator(Kenkyū-buntansha) OKUMURA Hirokazu  KANAZAWA UNIVERSITY, Graduate School of Med.Sci., Assistant Prof., 医学系研究科, 助手 (30242548)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPlasma DNA / PCR / Immunoglobulin gene / CDR3 / NHL / 非ホジキンリンパ腫
Research Abstract

In B cell lymphoid malignancies, malignant cells proliferate monoclonally with the same immunoglobulin gene rearrangement.This rearrangement can be detected by the use of the polymerase chain reaction(PCR).Recently, it has been reported that proliferation of clonal cells could be demonstrated by PCR analyzing DNA extracted from patients' plasma with B cell malignancies.The question, however, still remains whether amplified products were derived from tumor cells or not.In this study, using DNA extracted from both malignant cells and plasma of the same patients with B cell leukemia, I attempted to amplify immunoglobulin heavy chain(IgH) complementarity determining region 3(CDR3) by semi-nested PCR and to analyze the sequences respectively.The results showed that sequences of DNA from plasma were identical with that of DNA from tumor cells in all cases.In addition, some of them showed homology to CDR3 that was previously reported.These findings indicate that plasma DNA is released from tumor cells.In the next stage, it is necessary to raise the sensitivity, and if it works, IgH-PCR using plasma DNA might be useful tool for diagnosing patients and evaluating the efficacy of treatment, especially in patients with critical condition under which any biopsies were difficult.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Zhu J, Okumura H, Ohtake S et al.: "The molecular mechanism of arsenic trioxide-induced apoptosis and oncosis in leukemia/lymphoma cell lines"Acta Haematol.. 110. 1-10 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki R, Ohtake S, et al.: "Prognostic significance of CD7+CD56+ phenotype and chromosome 5 abnormalities for acute myeloid leukemia MO."Int J Hematol.. 77. 482-489 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhu J, Okumura H, Ohtake S et al.: "Arsenic trioxide induces apoptosis in leukemia/lymphoma cell lines via the CD95/CD95L system"Oncol Rep.. 10. 705-709 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki Y: "Cytogenetic heterogeneity of acute myeloid leukaemia (AML) with trilineage dysplasia : Japan Adult Leukaemia Study Group-AML 92 study"Br J Haematol. 120. 56-62 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohtake S: "Chronic myelogenous leukemia with p190BCR-ABL expression : the missing link with monocytosis"Intern Med. 41. 1092-1093 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terasaki Y Okumura H, Ohtake S, et al.: "Accelerated telomere length shortening in granulocytes. A diagnostic marker for myeloproliferative diseases"Exp Hematol. 30. 1399-1404 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大野 竜三, 大竹茂樹, 他: "白血病・悪性リンパ腫治療プロトコール集"医薬ジャーナル社. 187 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohtake S: "Chronic myelogenous leukemia with p19OBCR-ABL expression : the missing link with monocytosis."Intern Med.. 41(12). 1092-1093 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terasaki Y, Okumura H, Ohtake S, Nakao S.: "Accelerated telomere length shortening in granulocytes. A diagnostic marker for myeloproliferative diseases."Exp Hematol.. 30(12). 1399-1404 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki Y, Ohtake S, et al.: "Cytogenetic heterogeneity of acute myeloid leukaemia(AML) with trilineage dysplasia : Japan Adult Leukaemia Study Group-AML 92 study."Br J Haematol.. 120(1). 56-62 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhu J, Okumura H, Ohtake S, et al.: "Arsenic trioxide induces apoptosis in leukemia/lymphoma cell lines via the CD95/CD95L system."Oncol Rep.. 10(3). 705-709 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki R, Ohtake S, et al.: "Prognostic significance of CD7+CD56+ phenotype and chromosome 5 abnormalities for acute myeloid leukemia MO."Int J Hematol.. 77(5). 482-489 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhu J, Okumura H, Ohtake S, et al.: "The molecular mechanism of arsenic trioxide-induced apoptosis and oncosisin leukemia/lymphoma cell lines."Acta Haematol.. 110(1). 1-10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ozaki J, Okumura, H, Ohtake S, et al.: "Refractory Crow-Fukase Syndrome (POEMS Syndrome)Successfully Treated with High-Dose Melphalan followed by Autologous Peripheral Blood Stem Cell Transplantation."Int J Hematol.. 79(1). 95-99 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhu J: "The molecular mechanism of arsenic trioxide-induced apoptosis and oncosis in leukemia/lymphoma cell lines"Acta Haematol. 110・1. 1-10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki R: "Prognostic significance of CD7+CD56+ phenotype and chromosome 5 abnormalities for acute myeloid leukemia MO"Int J Hematol. 77・5. 482-489 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhu J: "Arsenic trioxide induces apoptosis in leukemia/lymphoma cell lines via the CD95/CD95L system"Oncol Rep.. 10・3. 705-709 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyazaki Y: "Cytogenetic heterogeneity of acute myeloid leukaemia (AML) with trilineage dysplasia : Japan Adult Leukaemia Study Group-AML 92 study"Br J Haematol. 120・1. 56-62 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohtake S: "Chronic myelogenous leukemia with p190BCR-ABL expression the missing link with monocytosis"Intern Med. 41・12. 1092-1093 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Terasaki Y: "Accelerated telomere length shortening in granulocytes. A diagnostic marker for myeloproliferative diseases"Exp Hematol. 30・12. 1399-1404 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大野 竜三: "白血病・悪性リンパ腫治療プロトコール集"医薬ジャーナル社. 187 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jiebin Zhu: "The CD95/CD95L system mediates arsenic trioxide induced apoptosis in leukemia/lymphoma cell lines"Int j Cancer. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyazaki Y: "Cytogenetic heterogeneity of acute myeloid leukaemia (AML) with trilineage dysplasia : Japan Adult Leukaemia Study Group-AML 92 study"Br J Haematol. 120・1. 56-62 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohtake S: "Chronic myelogenous leukemia with p190BCR-ABL expression: the missing link with monocytosis"Intern Med.. 41・12. 1092-1093 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terasaki Y, Okumura H, Ohtake S, Nakao S.: "Accelerated telomere length shortening in granulocytes: a diagnostic marker for myeloproliferative diseases"Exp Hematol.. 30(12). 1399-1404 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeuchi J: "Induction therapy by frequent administration of doxorubicin with four other drugs, followed by intensive consolidation and maintenance therapy for adult acute lymphoblastic leukemia : the JALSG-ALL93 study"Leukemia. 16・7. 1259-1266 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大竹茂樹: "急性骨髄性白血病"臨床医. 28・10. 2107-2110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大竹 茂樹: "悪性リンパ腫免疫組織学的検査"診断と治療. 90・8. 1253-1258 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大竹 茂樹: "急性骨髄性白血病の薬物療法"Bio Clin. 120・1. 56-62 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大竹 茂樹: "急性リンパ性白血病の亜分類"血液・腫瘍科. 46・2. 136-142 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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