• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The significance of serum sphingosine 1-phospahte in hematological diseases and the regulatory mechanism of sphingosine kinase

Research Project

Project/Area Number 14572180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNagoya University

Principal Investigator

MURATE Takeshi  Nagoya University, School of Health Science, Professor, 医学部, 教授 (30239537)

Co-Investigator(Kenkyū-buntansha) KOIZUMI Keiko  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00118027)
TAKAGI Akira  Nagoya University, School of Health Science, Research Associate, 医学部, 助手 (30135371)
KOJIMA Tesuhito  Nagoya University, School of Health Science, Professor, 医学部, 教授 (40161913)
BANNNO Yoshiko  Gifu University School of Medicine, Gifu University School of Medicine, Associate Professor, 医学部, 助教授 (50116852)
SUZUKI Motoshi  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 講師 (80236017)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordssphingosine kinase 1 / polyclonal antibody / gene expression / promoter analysis / MEG-O1 / プロモータ解析 / スフィンゴシンキナーゼ
Research Abstract

As the preparatory step, the polyclonal antibody against 16 peptide-fragment of human sphingosine kinase 1 was made by immunizing rabbit. The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Protein kinase C inhibitor prevented the PMA-induced SPHK1 gene expression. we examined the promoter area (distal to the first exon) and its binding proteins. Luciferase analyses showed that the area of 300 bp from the first exon was sufficient for PMA-responsiveness, and that Sp1-and two AP-2-binding motifs within this area were necessary for responsiveness. Electrophoresis mobility shift assay showed that Sp1 protein was originally bound to the Sp1 site and that two additional bands bound to the two AP-2 motifs were observed only when stimulated with PMA in MEG-O1 cells. The appearance of these bands resulted from binding to an unknown protein rather than AP-2. These results indicated that PMA up-regulates SPHK1 gene expression through PMA-responsive elements of the 5' promoter area of the gene, and suggested that PMA-mediated SPHK1 gene expression would be mediated via PKC-and ERK-dependent signal transduction pathway by binding the transcription factor to AP-2 motifs.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Terada N. et al.: "Compartmentation of the mouse cerebellar cortex by spningosine kinase"J Comparative Neurol. 469. 119-127 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakada Y. et al.: "Regulation of spingosine kinase 1 gene expression by protein kinase C in a hyman leukemia cell line, MEG-O1."Biochim Biophys Acta. 1635. 104-116 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furuta M. et al.: "A novel platinum compound inhibits telomerase activity in vitro and reduces telomere length in a human epatoma cell line"Int J Cancer. 104. 709-715 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Limsirichaikul S. et al.: "The Gly-952 residue of Saccharomyces cerevisiae DNA polymerase alpha is important in discriminating correct deoxyribonucleotides from incorrect ones."J Biol Chem. 278. 19079-19086 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki E.et al.: "Expression levels of DNA methyltransferase genes do not correlate with P15INK4B gene methylation in myelodysplastic Syndromes."Leukemia. 17. 1903-1904 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murate T. et al.: "Up-regulation of acid sphingomyelinase during retinoic acid-induced myeloid differentiation of NB4, a human acute promyelocytic leukemia cell line"J Biol Chem. 277. 9936-9943 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada N.et al.: "Compartmentation of the mouse cerebellar cortex by sphingosine kinase"J Comparative Neurol. 469. 119-127 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakade Y.et al.: "Regulation of sphingosine kinase 1 gene expression by protein kinase C in a human leukemia cell line, MEG-01"Biochim Biophys Acta. 1635. 104-116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furuta M.et al.: "A novel platinum compound inhibits telomerase activity in vitro and reduces telomere length in a human hepatoma cell line."Int J Cancer. 104. 709-715 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Limsirichaikul S.et al.: "The Gly-952 residue of Saccharomyces cerevisiae DNA polymerase alpha is important in discriminating correct deoxyribonucleotides from incorrect ones."J Biol Chem. 278. 19079-19086 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki E.et al.: "Expression levels of DNA methyltransferase genes do not correlate with p15INK4B gene methylation in myelodysplastic syndromes."Leukemia. 17. 1903-1904 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murate T.et al.: "Up-regulation of acid sphingomyelinase during retinoic acid-induced myeloid differentiation of NB4, a human acute promyelocytic leukemia cell line."J Biol Chem. 277. 9936-9943 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada N. et al.: "Compartmentation of the mouse cerebellar cortex by sphingosine kinase"J Comparative Neurol. 469. 119-127 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakade Y. et al.: "Regulation of sphingosine kinase 1 gene expression by protein kinase C in a human leukemia cell line, MEG-O1"Biochim Biophys Acta. 1635. 104-116 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Furuta M. et al.: "A novel platinum compound inhibits telomerase activity in vitro and reduces telomere length in a human hepatoma cell line"Int J Cancer. 104. 709-715 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Limsirichaikul S. et al.: "The Gly-952 residue of Saccharomyces cerevisiae DNA polymerase alpha is important in discriminating correct deoxyribonucleotides from incorrect ones"J Biol Chem. 278. 19079-19086 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suyama T. et al.: "The MAGE-Al gene expression is not determined solely by methylation status of promoter region in meinatological malignuncies"Leuk Res. 12. 1113-1118 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Igarashi T. et al.: "Factors affecting toxicity, responce and progression-free surviveal in relpsed patients with indolent B-cell lymphoma and mintle cell lymphoma"Ann Oncol. 13. 928-943 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takagi A. et al.: "Involvement of caspase 3 mediated apoptosis in hematopoietic cytotoxicity of metabolites of ethylene glycol monomethyl ether"Industrial Health. 40. 371-374 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hurate T. et al.: "Up-regulation of acid sphingomyelinase during retinoic acid-induced myeloid differentiation of NB4, a human acute promyelocytic leukemia cell line"J. Biol. Chem. 277. 9936-9943 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2018-02-02  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi