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Development of molecular investigation technique using the newly identified renal organic acid transporter molecule (hyperuricemia and renal cell carcinoma).

Research Project

Project/Area Number 14572199
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOsaka Medical Center for Cancer and Cardiovascular Diseases (Research Institute)

Principal Investigator

NAKAJIMA Hiromu  Osaka Medical Center for Cancer and Cardiovascular Diseases (Research Institute), Osaka Medical Center for Cancer and Cardiovascular Diseases, Research Institute, Special Investigator (50252680)

Co-Investigator(Kenkyū-buntansha) MIKUNI Tomiko  大阪府立成人病センター(研究所), Osaka Medical Center for Cancer and Cardiovascular Diseases, Research Institute, Investigator (40332457)
松永 隆  大阪府立成人病センター(研究所), 病院臨床検査科, 医長
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsgene analysis / genomics / proteomics / organic acid transporter / epitope / gene cloning / specific antibody / cancer / 遺伝子構造 / mRNAスプライシング
Research Abstract

In the preceding research, we have cloned the human homologue (UAT) of rat renal organic acid transporter mRNA specific to uric acid renal transport. According to this result, we have investigated in this research project, the development of molecular investigation technique using the newly identified molecule with special reference to hyperuricemia and renal cell carcinoma. Following results have been obtained.
1. Cloning and determination of full-genomic sequence for human UAT using PAC human genomic library.
2. Determination of the exon-intron organization and genomic structure.
3. Determination of the promoter structure and its putative transcription factor binding sites.
4. Analysis of tissue specific alternative splicing.
5. Computer analysis of the secondary and the tirtially structure of this gene product.
6. Production of specific antibody using oligopeptide immunization technique.
In the consecutive research to this project, we use this antibody to the assay of this molecule and histochemical investigation for the analysis of pathophysiology and clinical evaluation of the patients with hyperuricemia or renal cell carcinoma.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (27 results)

All 2003 2002 Other

All Journal Article (12 results) Book (1 results) Publications (14 results)

  • [Journal Article] Identification of a novel variant in the phosphoenolpyruvate carboxykinase gene promoter in Japanese patienis with type 2 diabetes.2003

    • Author(s)
      Horikawa Y, Nakajima H, et al.
    • Journal Title

      Horm. Metab. Res. 35

      Pages: 308-312

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Determination of D-mannose in plasma by HPLC.2003

    • Author(s)
      Taguchi T, Nakajima H, et al.
    • Journal Title

      Clin. Chem. 49

      Pages: 181-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Mechanism of the production of reactive oxygen and nitrogen species in the stomach.2003

    • Author(s)
      Mikuni T, Nakajima H, et al.
    • Journal Title

      Curr. Topics Pharmacol. 8

      Pages: 67-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification of a novel variant in the phosphoenolpyruvate carboxykinasegene promoter in Japanese patients with type 2 diabetes.2003

    • Author(s)
      Horikawa Y, Nakajima H, et al.
    • Journal Title

      Horm Metab Res 35

      Pages: 308-312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Determination of D-mannose in plasma by HPLC.2003

    • Author(s)
      Taguchi T, Nakajima H, et al.
    • Journal Title

      Clin Chem 49

      Pages: 181-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Mechanism of the production of reactive oxygen and nitrogen species in the stomach.2003

    • Author(s)
      Mikuni T, Nakajima H, et al.
    • Journal Title

      Curr Topics Pharmacol 8

      Pages: 67-77

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Phosphofructokinase deficiency, past, present and future.2002

    • Author(s)
      Nakajima H, et al.
    • Journal Title

      Curr. Mol. Med. 2

      Pages: 197-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Radiosurgery for acromegaly.2002

    • Author(s)
      Saitou Y, Nakajima H, et al.
    • Journal Title

      Recent Res. Devel. Endocrinol. 3

      Pages: 1-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Metabolic consequence of long-term exposute of pancreatic beta cells to free fatty acid with special reference to glucose insensitivity.2002

    • Author(s)
      Iizuka K, Nakajima H, et al.
    • Journal Title

      Biochim. Biophys. Acta 1586

      Pages: 23-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Phosphofructokinase deficiency, past, present and future.2002

    • Author(s)
      Nakajima H, et al.
    • Journal Title

      Curr Mol Med 2

      Pages: 197-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Radiosurgery for acromegaly.2002

    • Author(s)
      Saitou Y, Nakajima H, et al.
    • Journal Title

      Recent Res Devel Endocrino. 3

      Pages: 1-10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Metabolic consequence of long-term exposure of pancreatic beta cells to free fatty acid with special reference to glucose insensitivity.2002

    • Author(s)
      Iizuka K, Nakajima H, et al.
    • Journal Title

      Biochim Biophys Acta 1586

      Pages: 23-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] かんじゃ想い2003

    • Author(s)
      中島弘、ほか
    • Total Pages
      306
    • Publisher
      日総研出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horikawa Y, Nakajima H, et al.: "Identification of a novel variant in the phosphoenolpyruvate carboxykinase gene promoter in Japanese patients with type 2 diabetes."Horm Metab Res. 35. 308-312 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taguchi T, Nakajima H, et al.: "Determination of D-Mannose in Plasma by HPLC."Clinical Chemistry. 49(1). 181-183 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mikuni T, Nakajima H, et al.: "Mechanism of the production of reactive oxygen and nitrogen species in the stomach"Current Topics in Pharmacology. 8(1). 67-77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakajima H: "The globalization of bioethics-A review of current conditions in Japan for the health care system in the 21st century-."Japan Med Assoc J. 47(2). 84-93 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中島 弘: "職域における高尿酸血症の管理:高尿酸血症・痛風の治療ガイドラインをふまえて"産衛誌. 45. 12-19 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中島 弘: "生活指導:高尿酸血症・痛風の治療ガイドラインにおける基本的な治療方針"痛風と核酸代謝. 27(2). 189-194 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中島 弘 ほか: "かんじゃ想い"日総研出版. 306 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakajima H., et al.: "Phosphofructokinase deficiency ; past, Present and future"Curr.Mol.Med.. 2(2). 197-212 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitou Y, Nakajima H, et al.: "Radiosurgery for acromegaly.(review)"Recent Res Devel Endocrinol. 3. 1-10 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iizuka K, Nakajima H, et al.: "Metabolic consequence of long-term exposure of pancreatic beta cells to free fatty acid with special reference to glucose insensitivity"Biochim Biophys Acta. 1586(1). 23-31 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mikuni T, et al.: "Production of hydroxyl free radical in the xanthine oxidase system with addition of 1-methyl-3nitro-1-nitrosoguanidine"Free Radic Res. 36(6). 641-647 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中島 弘 他: "E-CELLを用いたコンパートメントモデルによる糖尿病病態の再現"日本臨床分子医学会記録. 38. 39-40 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中島 弘: "「高尿酸血症・痛風の治療ガイドライン」にみる生活習慣病対策と尿路管理の考え方について"日本尿路結石症学会誌. 1(1). 39-56 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中島 弘 他: "高尿酸血症・痛風の治療ガイドライン(第一版)"日本痛風・核酸代謝学会編. (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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