| Project/Area Number |
14580015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
体育学
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| Research Institution | Chiba University |
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Principal Investigator |
KASUYA Yoshitoshi Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (70221877)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAUCHI Takashi Tsukuba Univ., Clinical Medical Sciences, Professor, 臨床医学系, 教授 (60222329)
NISHIYAMA Mariko Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (00092081)
KIMURA Sadao Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (40134225)
MATUDA Mituo Tsukuba Univ., Health, Physical and Sports Sciences, Professor, 体育科学系, 教授 (20110702)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | cardiac hypertrophy / PPAR-α / Fenofibrate / Endothelin(ET)-1 / rat cardiomyocytes / aortic banding model / MAP kinases / JNK |
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| Research Abstract |
We investigated possible roles of PPAR-α activation in ET-1-induced hypertrophy of cultured rat cardiomyocytes and in an in vivo model of cardiac hypertrophy (abdominal aortic banding : AB). Fenofibrate markedly inhibited the ET-1-induced increase in cell size, protein synthesis, expression level of c-Jun and phosphorylation level of c-Jun and JNK. PPAR-α overexpression inhibited ET-1-induced increase in protein synthesis. Fenofibrate significantly inhibited the ET-1-induced ET-1 mRNA expression, which was confirmed by luciferase assay. EMSA revealed that fenofibrate decreased AP-1 binding activity induced by ET-1 or PMA. Fenofibrate-treatment inhibited LV hypertrophy and phenotypic changes in cardiac gene expression in AB rats.
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