• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of acute exercise on activation of the calcineurin signaling pathway involved in development of slow muscle fibers

Research Project

Project/Area Number 14580055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 体育学
Research InstitutionKyorin University

Principal Investigator

HITOMI Yoshiaki  Kyorin University, School of Medicine, Research Associate, 医学部, 助手 (70231545)

Co-Investigator(Kenkyū-buntansha) OHNO Hideki  Kyorin University, School of Medicine, Professor, 医学部, 教授 (00133819)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsSkeletal muscle / Exercise / Slow muscle fiber / Fast muscle fiber / Calcineurin / MCIP1 / NFAT / Transcription / 速筋線維 / 遅筋繊維 / カルシュニューリン
Research Abstract

To investigate the role of the calcineurin in development of slow muscle fibers, we first compared the mRNA expression of genes in the calcineurin signaling pathway in slow muscle fibers with that in fast muscle fibers. It revealed that the mRNA expression of myocyte specific calcineurin inhibitory protein 1 (MCIP1) was at least 10 times higher in slow muscle fibers than in fast muscle fibers. The difference in the MCIP1 mRNA expression was lost, when slow and fast muscle fibers lost their differentiated phenotypes after denervation of skeletal muscles.
The expression of MCIP1 mRNA was transiently induced in skeletal muscles within 4 hours after acute exercise. In the MCIP1 promoter sequence, there is an array of tentative NFAT binding sites, which might respond to the calcineurin activity. Indeed, calcineurin directly regulated the MCIP1 promoter via these NFAT binding sites. Therefore, the expression levels of MCIP1 mRNA might directly reflect the calcineurin activity in skeletal muscle, which is otherwise very difficult to monitor in vivo.
We next examined the relationship between the intensity of acute exercise and the induction of MCIP1 mRNA in rat skeletal muscles, after treadmill running at various speed and time. The expression of MCIP1 mRNA was proportionally correlated with the intensity of acute exercise, and it was induced by very mild exercise (12 m/min for 10 min). Even when varying speed from 8〜16 m/min for 120 m, the MCIP1 mRNA was equally induced in rat skeletal muscles. These results suggest that the expression level of MCIP1 mRNA is a good indicator of the calcineurin activity in skeletal muscle, and even very mild exercise is enough to activate the calcineurin signaling pathway, which is important for development and maintenance of slow muscle fibers.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Ohno H: "Extracellular superoxide dismutase and lifestyle related diseases (Review)"Curr Top Pharmacol. 1. 1-15 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H: "Hematological responses in juveniles after training at moderate altitude"Adv Exerc Sports Physiol. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H: "We have two strategies to attain healthy ageing"Geriatr Gerontol Int. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takemasa T: "Simple method for the identification of oxidative fibers in skeletal muscle"Eur J Appl Physiol. 91. 357-359 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Y: "Effect of Acute Exercise on Expression of mRNA Involved in the Calcineurin Signaling Pathway in Human Skeletal Muscle"IUBMB Life. 55. 409-413 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Y: "Intermittent hypobaric hypoxia increases the ability of neutrophils to generate superoxide anion in humans"Clin Exp Pharmacol Physiol. 30. 659-664 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H: "Physical exercise and extracellular superoxide dismutase : Antiarthrogenic effects"Adv Exerc Sports Physiol. 9. 45-53 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda H: "Enhanced expression of neuronal nitric oxide synthase in islets of exercise-trained rats"Biochem Biophys Res Commun. 19. 794-800 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K: "Genetic variation in hypoxia-inducible factor lalpha and its possible association with high altitude adaptation in Sherpas"Med Hypotheses. 61. 385-389 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimoto K: "Gliclazide protects pancreatic beta-cells from damage by hydrogen peroxide"Biochem Biophys Res Commun. 303. 112-119 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki H: "Effect of eccentric exercise on neutrophil function in human plasma"Adv Exerc Sports Physiol. 9. 69-75 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kizaki T: "Uncoupling protein 2 plays an important role in nitric oxide production of lipopolysaccharide-stimulated macrophages"Proc Natl Acad Sci USA. 99. 9392-9397 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Muto M: "Association between aldehyde dehydrogenase gene polymorphisms and the phenomenon of field cancerization in patients with head and neck cancer"Carcinogenesis. 23. 1759-1765 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato K: "Hyperstable U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells"Br J Cancer. 87. 898-904 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ookawara T: "Transcription regulation of gene expression in human skeletal muscle in response to endurance training"Res Commun Mol Pathol Pharmacol. 111. 41-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K: "Association of SH2-B to phosphorylated tyrosine residues in the activation loop of TrkB"Res Commun Mol Pathol Pharmacol. 111. 27-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 人見嘉哲: "トレーニング生理学"杏林書院. 375 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H, Kizaki T, Suzuki K, Hitomi Y, Watanabe S, Ookawara T, Suzuki K, Haga S, Saitoh D, Radak Z, Ji LL.: "Extracellular superoxide dismutase and lifestyle related diseases (Review)."Curr Top Pharmacol. 1. 1-15 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H, Kim CK, Kim JH, Jung YS, Bae SY, Toshinai K, Miyazaki H, Esaki K, Takemasa T, Kinugasa T, Kizaki T, Hitomi Y, Ookawara T, Oh-ishi S, Haga S.: "Hematological responses in juveniles after training at moderate altitude."Adv Exerc Sports Physiol. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H, Kizaki T, Hitomi Y, Watanabe S, Ishibashi Y, Izawa T, Saitoh D, Kinugasa T, Haga S.: "We have two strategies to attain healthy ageing."Geriatr Gerontol Int. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takemasa T, Sugimoto K, Miyazaki M, Machida M, Ikeda S, Hitomi Y, Kizaki T, Ohno H, Yamashita K, Haga S.: "Simple method for the identification of oxidative fibers in skeletal muscle."Eur J Appl Physiol. 91. 357-359 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Y, Kizaki T, Katsumura T, Mizuno M, Itoh CE, Esaki K, Fujioka Y, Takemasa T, Haga S, Ohno H.: "Effect of moderate acute exercise on expression of mRNA involved in the calcineurin signaling pathway in human skeletal muscle."IUBMB Life. 55. 409-413 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Y, Miyamura M, Mori S, Suzuki K, Kizaki T, Itoh C, Murakami K, Haga S, Ohno H.: "Intermittent hypobaric hypoxia increases the ability of neutrophils to generate superoxide anion in humans."Clin Exp Pharmacol Physiol. 30. 659-664 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno H, Kizaki T, Suzuki K, Hitomi Y, Watanabe S, Ookawara T, Suzuki K, Haga S, Saitoh D, Radak Z, Ji LL.: "Physical exercise and extracellular superoxide dismutase : antiatherogenic effects (Review)."Adv Exerc Sports Physiol. 9. 45-53 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda H, Urano Y, Sakurai T, Kizaki T, Hitomi Y, Ohno H, Izawa T.: "Enhanced expression of neuronal nitric oxide synthase in islets of exercise trained rats."Biochem Biophys Res Commun. 312. 794-800 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K, Kizaki T, Hitomi Y, Nukita M, Kimoto K, Miyazawa N, Kobayashi K, Ohnuki Y, Ohno H.: "Genetic variation in hypoxia-inducible factor lalpha and its possible association with high altitude adaptation in Sherpas."Med Hypotheses. 61. 385-389 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki H, Haga S, Suzuki K, Toshinai K, Esaki K, Oh-ishi S, Hitomi Y, Kizaki T, Ji LL, Ohno H.: "Effect of eccentric exercise on neutrophil function in human plasma."Adv Exerc Sports Physiol. 9. 69-75 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kizaki T, Suzuki K, Hitomi Y, Taniguchi N, Saitoh D, Watanabe K, Onoe K, Day NK, Good RA, Ohno H.: "Uncoupling protein 2 plays an important role in nitric oxide production of lipopolysaccharide-stimulated macrophages."Proc Natl Acad Sci USA. 99. 9392-9397 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Muto M, Nakane M, Hitomi Y, Yoshida S, Sasaki S, Ohtsu A, Yoshida S, Ebihara S, Esumi H.: "Association between aldehyde dehydrogenase gene polymorphisms and the phenomenon of field cancerization in patients with head and neck cancer."Carcinogenesis. 23. 1759-1765 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato K, Hitomi Y, Imamura K, Esumi H.: "Hyperstable U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells."Br J Cancer. 87. 898-904 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ookawara T, Suzuki K, Haga S, Ha S, Chung KS, Toshinai K, Hamaoka T, Katsumura T, Takemasa T, Mizuno M, Hitomi Y, Kizaki T, Suzuki K, Ohno H.: "Transcription regulation of gene expression in human skeletal muscle in response to endurance training."Res Commun Mol Pathol Pharmacol. 111. 41-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K, Mizutani M, Hitomi Y, Kizaki T, Ohno H, Ishida H, Haga S, Koizumi S.: "Association of SH2-B to phosphorylated tyrosine residues in the activation loop of TrkB."Res Commun Mol Pathol Pharmacol. 111. 27-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitomi Y: "Effect of Acute Exercise on Expression of mRNA Involved in the Calcine urin Signaling Pathway in Human Skeletal Muscle"IUBMB Life. 55. 409-413 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hitomi Y: "Intermittent hypobaric hypoxia increases the ability of neutrophils to generate superoxide anion in humans"Clin Exp Pharmacol Physiol. 30. 659-664 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohno H: "Physical exercise and extracellular superoxide dismutase : Antiarthrogenic effects"Adv Exerc Sports Physiol. 9. 45-53 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemasa T: "Simple method for the identification of oxidative fibers in skeletal muscle"Eur J Appl Physiol. 91. 357-359 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueda H: "Enhanced expression of neuronal nitric oxide synthase in islets of exercise-trained rats"Biochem Biophys Res Commun. 19. 794-800 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki K: "Genetic variation in hypoxia-inducible factor lalpha and its possible association with high altitude adaptation in Sherpas"Med Hypotheses. 61. 385-389 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 人見嘉哲: "トレーニング生理学"杏林書院. 375 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kizaki, T. et al.: "Uncoupling protein 2 plays an important role in nitric oxide production of lipopolysaccharide-stimulated macrophage"Proc. Natl. Acad. Sci. USA. 99. 9392-9397 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Muto, M. et al.: "Association between aldehyde dehydrogenase gene polymorphisms and the phenomenon of field cancerization in patients with head and neck cancer"Carcinogenesis. 23. 1759-1765 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kato, K. et al.: "Hyperstable U1snRNA complementary to・the K-ras transcripts induces cell death in pancreatic cancer cells"Br. J Cancer. 87. 898-904 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohno, H. et al.: "Physical exercise and extracellular superoxide dismutase : antiatherogenic effects"Adv. Exerc. Sports Physiol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimoto, K. et al.: "Gliclazide protects pancreatic beta-cells from damages by hydrogen peroxide"Biochem. Biophys. Res. Commun.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohno, H. et al.: "Extracellular superoxide dismutase and lifestyle related diseases"Curr. Top. Pharmacol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 人見嘉哲: "トレーニング生理学"杏林書院. 375 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi