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ROLE OF BRCA1 IN RADIATION-INDUCED CENTROSOMAL CHECKPOINT

Research Project

Project/Area Number 14580569
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionNagasaki University

Principal Investigator

SUZUKI Kenji  Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Research Associate, 大学院・医歯薬学総合研究科, 助手 (00196809)

Co-Investigator(Kenkyū-buntansha) WATANABE Masami  Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Professor, 大学院・医歯薬学総合研究科, 教授 (20111768)
KODAMA Seiji  Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (00195744)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsRADIATION / BRCA1 / CENTEROSOME / PHOSPHORYLATION
Research Abstract

IONIZING RADIATION 'HAS BEEN KNOWN TO INDUCED DNA DOUBLE STRAND BREAKS, WHICH RESULT IN ACTIVATION OF ATM. ACTIVATED ATM PHOSPHORYLATES VARIOUS PROTEINS INCLUDING BRCA1. BRCA1 HAS BEEN FOUND TO BE INVOLVED IN INHERITED MAMMARY CARCINOMA, AND IT IS THOUGHT TO HAVE A FUNCTION IN REPAIR OF DNA DOUBLE STRAND BREAKS. HERE, WE SHOW THAT BRCA1 COLOCALIZE WITH CENTROSOMAS. WITHOUT BRCA1 CENTROSOMES TEND TO OVERAMPLIFY, WHICH LEAD TO MULTIPOLAR DIVISION. FURTHERMORE, WE FOUND THAT ACTIVATED FORMS OF ATM, PHOSPHORYLATED ATM AT SERINE1981, ALSO COLOCALIZED WITH CENTROSOMES, WHICH INDICATE THAT ACTIVATED ATM CONSTITUTIVELY PHOSPHORYLATE BRCA1. THESE RESULTS INDICATE THAT ATM-MEDIATED PHOSPHORYLATION OF BRCA1 MAY REGULATE CENTROSOMAL DUPLICATION NOT ONLY IN _UNIRRADIATED CELLS BUT ALSO AFTER IRRADIATION.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Keiji Suzuki: "Delayed reactivation of p53 in the progeny of cells surviving ionizing radiation"Cancer Research. 63. 936-941 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Suzuki: "Radiation-induced DNA damage and delayed induced genomic instability"Oncogene. 22. 6988-6993 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Genro Kashino: "Relief of oxidative stress by ascorbic acid delayes cellular senescence of normal human and Werner syndrome fibroblast cells"Free Radical and Biological Medicine. 35. 438-443 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mana Miyakoda: "Activation of ATM and phosphorylation of p53 by heat shock"Oncogene. 21. 1090-1096 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Nakahata: "Heat shock induces centrosomal dysfunction, and causes non-apoptotic mitotic catastrophe in human tumor cells"International Journal of Hyperthermia. 18. 332-343 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kumio Okaichi: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma cells through the p53 pathway"Oncology Report. 9. 859-862 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Suzuki, M.Ojima, S.Kodama, M.Watanabe.: "Radiation-induced DNA damage and delayed induced genomic instability"Oncogene. 22. 6988-6993 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Suzuki, S.Yokoyama, S.Waseda, S.Kodama, M.Watanabe: "Delayed reactivation of p53 in the progeny of cells surviving ionizing radiation."Cancer Res.. 63. 936-941 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Hamada, S.Kodama, K.Suzuki, M.Watanabe.: "Gap junctional intercellular communication and cellular response to heat stress."Carcinogenesis. 24. 1723-1728 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] G.Kashino, S.Kodama, Y.Nakayama, K.Suzuki, K.Fukase, M.Goto, M.Watanabe.: "Relief of oxidative stress by ascorbic acid delays cellular senescence of normal human and Werner syndrome fibroblast cells"Free Radic.Biol.Med.. 35. 438-443 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Miyakoda, K.Suzuki, S.Kodama, M.Watanabe: "Activation of ATM and phosphorylation of p53 by heat shock."Oncogene. 21. 1090-1096 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakahata, M.Miyakoda, K.Suzuki, S.Kodama, M.Watanabe: "Heat shock induces centrosomal dysfunction, and causes non-apoptotic mitotic catastrophe in human tumor cells."Int.J.Hyperthermia. 18. 332-343 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Okaichi, K.Suzuki, N.Morita, M.Ikeda, H.Takahashi, N.Matsuda, M.Watanabe, Y.Okumura: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma cells through the p53 pathway."Oncol.Re. 9. 859-862 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Suzuki: "Delayed reactivation of p53 in the progeny of cells surviving ionizing radiation"Cancer Research. 63. 936-941 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiji Suzuki: "Radiation-induced DNA damage and delayed induced genomic instability"Oncogene. 22. 6988-6993 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mana Miyakoda: "Activation of ATM and phosphorylation of p53 by heat shock"Oncogene. 21. 1090-1096 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kumio Okaichi: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma Cells through the p53 pathway"Oncology Reports. 9. 859-862 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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