| Project/Area Number |
14580576
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
環境影響評価(含放射線生物学)
|
|---|
| Research Institution | Kisarazu National College of Technology |
|---|
Principal Investigator |
FUKUCHI Ken'ichi Kisarazu National College of Technology, Natural Science, Associate Professor, 基礎学系, 助教授 (00218942)
|
|---|
| Project Period (FY) |
2002 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
|
|---|
| Keywords | ultraviolet stress / ozone stress / fluorescence in plant leaves / laser-induced fluorescence / LIF spectra / UV-LED / 紫外線 / オゾン / 植物蛍光 / イメージング分光法 / 蛍光の葉内分布 / UV-B / 植物診断 |
|---|
| Research Abstract |
The influence of ultraviolet (UV) rays and air pollution gases exerted on plant growth was investigated by measurement of the UV laser excited LIF (Laser-induced fluorescence) spectra in peanuts (Arachis hypogaea L.) leaves. The following results were obtained.
1)The value of R_<685/530>, fluorescence intensity ratio of 685 nm (F685) and 530 nm (F530), is proportional to both logarithm of ozone density C (ppm) and exposure time T(h) ; R_<685/530>=1-(0.346×lnT+0.958)×C.
2)The value of R_<685/530> decreases in proportion to the UV-B fluence (Product of UV intensity I (Wm^<-2>) and exposure time T (h) ; R_<685/530>=1-0.0375×I×T.
3)Changing of R_<740/685>, fluorescence intensity ratio of 740 nm (F740) and 685 nm (F685), is different in UV-B irradiation and ozone exposure.
From the above-mentioned, it is understood that to detect early damage of UV stress and ozone stress is possible by the UV-LIF method.
In order to measure all wavelength of the fluorescence distribution in leaf simultaneously, ISA (Imaging spectrogram analysis) device was newly developed. The following results were obtained by ISA measurements.
4)The chlorophyll fluorescence ratio (F740/F685) increased according to the depth from the surface in control leaf, while it maintained a constant value in the UV-B damaged leaf.
5)Blue fluorescence (400nm - 500nm) increased remarkably under UV-B irradiation in the upper epidermis of the leaf. It is thought that ISA device is useful for examining the growth condition of plant leaves.
Moreover, the following became clear since the compound influence of the stress was evaluated based on the above-mentioned finding.
6)UV-A irradiation to the control leaves usual hardly influences the revitalization decrease, while a remarkable damage is caused to the UV-B damaged leaves.
|
