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Study of Mutations in Boundary Regions of GC% Mosaic Domains On Human Genome Using Microarray-CGH technique

Research Project

Project/Area Number 14580579
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionRadiation Effects Research Foundation

Principal Investigator

KODAIRA Mieko  , 財団法人・放射線影響研究所・遺伝学部, 副主任研究員 (60344412)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Norio  , 財団法人・放射線影響研究所・遺伝学部, 副部長(研究員) (40333546)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmicroarray-CGH technique / GC% transition regions in human genome / DNA-conformation / radiation / deletion mutation / ヒトゲノムGC含量境界領域 / 突然変異 / HPRT遺伝子
Research Abstract

It is suggested that the sharp GC% transition regions between different components of megabase-level mosaic domains named isochors (hereinafter as referred to as "the boundary regions") construct special conformations and thus they are likely to have insertion-, deletion-, and rearrangement-type mutations. In this study, we elucidated the genetic instability and the radiation effects on these boundary regions in the somatic cells and germ cells.
1.We isolated 22 radiation-induced HPRT-deficients with large deletions and identified the range of deletions around the HPRT locus by PCR. The GC% distribution shows one possible "boundary region" around 4〜4.5 Mb from 5' of the HPRT, but most of deletion ends don't exist on this boundary. We also analyzed 7 clones for identifying the deletion and duplications by using the array-CGH method. The arrays for this analysis contain BAG clones distributed every 1.5 Mb on chromosome 5 to 22 and clones covering about 8 Mb of chromosome 11 containing two "boundary regions". We detected 5 large deletions (15 to>20 Mb) in 3 of 7 clones, but no deletion end was mapped on the boundary region identified on chromosome 11. We found 3 deletions extend from the "possible boundary region" suggested by the analysis of GC% distribution.
2.We also screened DNA from 80 children of A-bomb survivors by array-CGH analysis using the arrays containing 2300 BAG clones from chromosome 1 to 22 and 66 BAG clones covering two "boundary regions" on chromosome 11,but we didn't find any de novo mutations among them.
Although this result cannot show the evidence that radiation-induced deletions are mainly triggered by DNA damages on the boundary regions, it is still important to study the difference of mutation, spectrum between genome units having specific structures.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (40 results)

All 2004 2003 2002 Other

All Journal Article (34 results) Publications (6 results)

  • [Journal Article] 放射線照射で生じたヒト・HPRT(ヒポキサンチン-グアニンフォスフォリボシルトランスフェラーゼ)遺伝子座の大きな欠失解析をモデルとしたマイクロアレイ-CGH(comparative Genomic Hybridization)法の特徴付け2004

    • Author(s)
      小平美江子 他
    • Journal Title

      広島医学 57(4)

      Pages: 417-421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical surveillance of surgical imipenem-resistant Pseudomonas aeruginosa infection in a Japanese hospital2004

    • Author(s)
      Sasaki M et al.
    • Journal Title

      J Hosp Infect 56(2)

      Pages: 111-118

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] LTA252G allele containing haplotype block is associated with high serum C-reactive protein levels2004

    • Author(s)
      Suzuki G et al.
    • Journal Title

      Atherosclerosis 176(1)

      Pages: 91-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 広範囲 血液・尿化学検査,免疫学的検査.その数値をどう読むか(第6版)VII.血球化学検査:ホスホグルコムターゼ(PGM)2004

    • Author(s)
      高橋規郎
    • Journal Title

      日本臨牀 62巻増刊号12

      Pages: 804-808

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A genome scanning approach to assess the genetic effects of radiation in mice and humans2004

    • Author(s)
      Asakawa J et al.
    • Journal Title

      Radiation Research 161(4)

      Pages: 380-390

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] No Evidence of Radiation Effect on Mutation Rates at Hypervariable Minisatellite Loci in the Germ Cells of Atomic Bomb Survivors2004

    • Author(s)
      Kodaira M.et al.
    • Journal Title

      Radiation Research 162(4)

      Pages: 350-356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of microarray based comparative genomic hybridization technique using model analysis of radiation-induced large deletions at the human HPRT (hypoxanthine guanine phosphoribosyl transferase) locus.2004

    • Author(s)
      Kodaira M et al.
    • Journal Title

      J Hiroshima Med Assoc 57

      Pages: 417-421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical surveillance of surgical imipenem-resistant Pseudomonas aeruginosa infection in a Japanese hospital.2004

    • Author(s)
      Sasaki M et al.
    • Journal Title

      J Hosp Infect 56

      Pages: 111-118

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] LTA252G allele containing haplotype block is associated with high serum C-reactive protein levels.2004

    • Author(s)
      Suzuki G et al.
    • Journal Title

      Atherosclerosis 176

      Pages: 91-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A genome scanning approach to assess the genetic effects of radiation in mice and humans.2004

    • Author(s)
      Asakawa J et al.
    • Journal Title

      Radiat Res 161

      Pages: 380-390

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] No evidence of radiation effect on mutation rates at hypervariable minisatellite loci in the germ cells of atomic bomb survivors.2004

    • Author(s)
      Kodaira M et al.
    • Journal Title

      Radiat Res 162

      Pages: 350-356

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Genome Scanning Approach to Assess the Genetic Effects of Radiation in Mice and Humans2004

    • Author(s)
      Jun-ichi Asakawa
    • Journal Title

      Radiation Research 161

      Pages: 380-390

    • Related Report
      2004 Annual Research Report
  • [Journal Article] No Evidence of Radiation Effect on Mutation Rates at Hypervariable Minisatellite Loci in the Germ Cells of Atomic Bomb Survivors2004

    • Author(s)
      Mieko Kodaira
    • Journal Title

      Radiation Research 162

      Pages: 350-356

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 広範囲 血液・尿化学検査、免疫学的検査、その数値をどう読むか(第6版)VII.血球化学検査:ホスホグルコムターゼ(PGM)2004

    • Author(s)
      高橋 規郎
    • Journal Title

      日本臨牀 62巻(増刊号)

      Pages: 804-808

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Study for genetic effects of atomic-bomb radiation by using of a DNA microarray-based comparative genomic hybridization (array-CGH) method2003

    • Author(s)
      Takahashi et al.
    • Journal Title

      12th International Congress of Radiation Research Book of Abstracts (eds R Cooper, G Dickie, D Hill, M Lavin, R Martin, A Whittaker, D Mather)

      Pages: 196-196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of human HPRT-deletion mutants by the microarray-CGH (Comparative genomic hybridization)2003

    • Author(s)
      Kodaira M et al.
    • Journal Title

      12th International Congress of Radiation Research Book of Abstracts (eds R Cooper, G Dickie, D Hill, M Lavin, R Martin, A Whittaker, D Mather)

      Pages: 196-196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Study for genetic effects of atomic-bomb radiation by using of a DNA microarray-based comparative genomic hybridization (array-CGH) method.2003

    • Author(s)
      Takahashi N et al.
    • Journal Title

      12th International Congress of Radiation Research Book of Abstracts (eds R Cooper, G Dickie, D Hill, M Lavin, R Martin, A Whittaker, D Mather)

      Pages: 196-196

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of human HPRT-deletion mutants by the microarray-CGH (comparative genomic hybridization).2003

    • Author(s)
      Kodaira M et al.
    • Journal Title

      12th International Congress of Radiation Research Book of Abstracts (eds R Cooper, G Dickie, D Hill, M Lavin, R Martin, A Whittaker, D Mather)

      Pages: 196-196

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of a DNA microarray-based comparative genomic hybridization (Array-OGH) method to the study on the genetic effects of Atomic bomb radiation ; Report 2.2002

    • Author(s)
      Takahashi N et al.
    • Journal Title

      Am J Hum Genet 71

      Pages: 407-407

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNAマイクロアレイのCGH法への適用の試み2002

    • Author(s)
      佐々木圭子
    • Journal Title

      広島医学 55(3)

      Pages: 280-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic effects of atomic-bomb radiation : Analysis of minisatellite genes2002

    • Author(s)
      Kodaira M
    • Journal Title

      Proceedings of the Workshop : Recognition of DNA damage as onset of successful repair : Computational and experimental approaches (ed M Pinak)

      Pages: 43-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ヒト・ミニサテライトでの突然変異率への影響を指標とした放射線の遺伝的影響2002

    • Author(s)
      小平美江子
    • Journal Title

      放射線生物研究 37(3)

      Pages: 264-281

    • NAID

      40005507798

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ヒトにおける放射線の遺伝的影響:ミニサテライト遺伝子座における突然変異2002

    • Author(s)
      小平美江子
    • Journal Title

      長崎医学会雑誌 77

      Pages: 188-191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 原爆放射線の遺伝的影響を効率良く調査するための方法-DNAマイクロアレイ法の導入2002

    • Author(s)
      高橋規郎
    • Journal Title

      長崎医学会雑誌 77

      Pages: 192-197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] マイクロアレイcomparative genomic hybridization(アレイCGH)法を用いた原爆放射線の遺伝的影響調査-予備実験2002

    • Author(s)
      佐々木圭子 他
    • Journal Title

      長崎医学会雑誌 77

      Pages: 316-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Two-dimensional complementary deoxyribonucleic acid electrophoresis revealing up-regulated human epididymal protein-1 and down-regulated CL-100 in thyroid papillary carcinoma2002

    • Author(s)
      Asakawa J et al.
    • Journal Title

      Endocrinelogy 143(11)

      Pages: 4422-4428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of a DNA microarray-based comparative genomic hybridization (Array-CGH) method to the study on the genetic effects of atomic bomb radiation ; Report 2.2002

    • Author(s)
      Takahashi N et al.
    • Journal Title

      Am J Hum Genet 71

      Pages: 407-407

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Application of microarray system to CGH.2002

    • Author(s)
      Sasaki K et al.
    • Journal Title

      J Hiroshima Med Assoc 55

      Pages: 280-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic effects of atomic-bomb radiation : Analysis of minisatellite genes.2002

    • Author(s)
      Kodaira M.
    • Journal Title

      Proceedings of the Workshop : Recognition of DNA damage as onset of successful repair : Computational and experimental approaches (ed M Pinak)

      Pages: 43-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The evaluation of genetic risk : Radiation effects on germline mutation rates at minisatellite loci.2002

    • Author(s)
      Kodaira M.
    • Journal Title

      Radiat Biol Res Commun 37

      Pages: 264-281

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic Effects of Radiation : Human Germline mutation rates at minisatellite loci.2002

    • Author(s)
      Kodaira M.
    • Journal Title

      Nagasaki Med J 77

      Pages: 188-191

    • NAID

      110000374178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Methodology to study genetic effects of A-bomb radiation efficiently. -Introduction of microarray-based comparative genome hybridization-.2002

    • Author(s)
      Takahashi N.
    • Journal Title

      Nagasaki Med J 77

      Pages: 192-197

    • NAID

      110000374179

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Study of genetic effects of atomic-bomb radiation by using microarray-based comparative genomic hybridization (array CGH). -Preliminary experiment-.2002

    • Author(s)
      Sasaki K et al.
    • Journal Title

      Nagasaki Med J 77

      Pages: 316-320

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Two-dimensional complementary deoxyribonucleic acid electrophoresis revealing up-regulated human epididymal protein-1 and down-regulated CL-100 in thyroid papillary carcinoma.2002

    • Author(s)
      Asakawa J et al.
    • Journal Title

      Endocrinology 143

      Pages: 4422-4428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Asakawa J.: "A Genome Scanning Approach to Assess the Genetic Effects of radiation in Mice and Humans"Radiation Research. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sasaki M.: "Clinical Surveillance of surgical imipenem-resistant Pseudomonas aeruginosa infection in a Japanese hospital"Journal of Hospital Infection. 56. 111-118 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小平美江子: "放射線照射で生じたヒト・HPRT(ヒポキサンチン-グアニンフォスフォリボシルトランスフェラーゼ)遺伝子座の大きな欠失解析をモデルとしたマイクロアレイ-CGH (Comparative Genomic Hybridization)法の特徴付け"広島医学. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kodaira M.: "Analysis of human HPRT-deletion mutants by the microarray-CGH (comparative genomic hybridization)"Australian Institute of Nuclear Science and Engineering. 1 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi N.: "Study for genetic effects of atomic-bomb radiation by using of a DNA microarray-based comparative genomic hybridization (array-CGH) method"Australian Institute of Nuclear Science and Engineering. 1 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐々木 圭子: "マイクロアレイcomparative genomic hybridization(アレイCGH)法を用いた原爆放射線の遺伝的影響調査-予備実験-"長崎医学会雑誌. 77・原爆特集号. 316-320 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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