| Project/Area Number |
14580608
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioorganic chemistry
|
|---|
| Research Institution | The University of Electro-Communications |
|---|
Principal Investigator |
NIWA Haruki The University of Electro-Communications, Faculty of Electro-Communications Applied Physics and Chemistry, Professor, 電気通信学部, 教授 (20135297)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRANO Takashi The University of Electro-Communications, Faculty of Electro-Communications Applied Physics and Chemistry, Associate Professor, 電気通信学部, 助教授 (20238380)
MAKI Shojiro The University of Electro-Communications, Faculty of Electro-Communications Applied Physics and Chemistry, Research Associate, 電気通信学部, 助手 (20266349)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | Bioluminescence / Odontosyllis / Plocamopherus imperialis / Mycea chlorophos / luciferin / luciferase / firefly / imidazopilazinone / 発光生物 / ヨコスジタマキビモドキ / アミノピラジン / Odontosyllis / 単離 |
|---|
| Research Abstract |
Molecular basis of light emitting system of various bioluminescent organisms were investigated. The bioluminescence system of the marine sea fire worm Odontosyllis undecimdonta was found to exhibit typical luciferin-luciferase reaction. The luciferin was found to be able to extract with methanol, and the luciferase with hot water. The emition of bluewish green light was observed by mixing the methanol extract and the water extract. The isolation of luciferin from the methanol extract was attempted by using HPLC, and we found that the luciferin to be acidic substance(s) with high polarity. Owing to low quantity of the luciferin content, we could not determine the chemical structure so far.
The bioluminescence system of the sea hare Plocamopherus imperialis was found also o exhibited luciferin-luciferase reaction. The sample was found to keep the bioluminescence ability even after storing at low temperature (below-80℃) for years.
In connection of exploration of bioluminescence system of th
… More
e mushroom Mycea chlorophos, we isolated basidiospores from the fruit-body of the mushroom collected from Hatijuo Island. The basidospores were germinated, from which a single strain of the fruiting mycelium was isolated. Using the single strain of the fruiting mycelium, we succeeded laboratory cultivation of fruiting body. We attempted the isolation of fluorescent substance(s) with the maximum around green light(510nm), resulting to the identification of lumichrom.
In the course of the construction of Bioluminescence Resonance Energy Transfer (BRET) system utilizing the firefly bioluminescence system coupled with Aequorea green fluorescence protein (GFP), the molecular design of blue light-producing firefly luciferin analogs was performed. After brief studies on the structure-bioluminescence activity relation ship, we succeeded the synthesis of an analog having a bioluminescence maximum shorter than that of native firefly luciferin.
In connection with the development of new photonics materials based on bioluminescence systems, we succeeded new imidazopilazinone derivatives with extended π-systems. Less
|
