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CONSTRUCTION OF A NEW ENZYME SYSTEM BY CHEMICAL MODIFICATION WITH VARIOUS AROMATIC COMPOUNDS

Research Project

Project/Area Number 14580610
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionKobe University

Principal Investigator

UEJI Shin-ichi  Kobe University, KOBE UNIVERSITY, FACULTY OF HUMAN DEVELOPMENT, PROFESSOR (40031364)

Co-Investigator(Kenkyū-buntansha) EBARA Yasuhito  KOBE UNIVERSITY, FACULTY OF HUMAN DEVELOPMENT, ASSOCIATE PROFESSOR (40251657)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsenzyme / chemical modification / enantioselectivity
Research Abstract

(1) From the investigation of FT-IR spectra, the chemical modification of Candida rugosa lipase with the aromatic compounds such as benzyloxycarbonyl (Z) and p-nitrobenzyloxycarbonyl Z(NO_2) moieties was found to cause a decrease of the α-helical structure of lipase.
(2) The lipase modified with Z group brought about a 10-fold increase in the enantioselectivity (E value) towards the hydrolysis of racemic butyl 2-(4-substituted phenoxy) propionates in aqueous buffer solution and the esterification of racemic 2-(4-substituted phenoxy) propionic acids with 1-butanol in organic solvents, although the enzyme activity was decreased. The observed order in the enantioselectivity is as follows ; Z>Z(NO_2)~Z(OCH_3). This enhancement effect of the chemical modification upon the E value is relatively small for the case of the chemical modification of lipase with t-butoxycarbonyl, lauoryl, and acetyl moieties.
(3) The origin of the enantioselectivity enhancement by the chemical modification was attributed to a significant deceleration in the initial rate for the incorrectly binding enantiomer.
(4) The optimal modified lipase displaying the highest enantioselectivity existed among the obtained lipase with various degree of the modification. The modification degree was estimated from the TNBS method.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (4 results)

All 2003 Other

All Journal Article (2 results) Publications (2 results)

  • [Journal Article] Chemical moclification of lipases with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions2003

    • Author(s)
      Shin-ichi Ueji
    • Journal Title

      Biotechnology Letters 25・1

      Pages: 83-87

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Chemical modification of lip as with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions2003

    • Author(s)
      Shin-ichi Ueji, et al
    • Journal Title

      Biotechnology Letters vol.25

      Pages: 83-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin-ichi Ueji: "Chemical modification of lipases with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions"Biotechnology Letters. 25・1. 83-87 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shin-ichi Ueji: "Chemical modification of lipases with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions"Biotechnology Letters. 25・1. 83-87 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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